Figure 1From: A microarray analysis of gnotobiotic mice indicating that microbial exposure during the neonatal period plays an essential role in immune system developmentOverview of the experimental procedure for bacterial reconstitution. During the experiment, all mice (including SPF mice) were housed in a Trexler-type flexible film isolator in a standard germ- free state. For generation of 0WexGF (mice, i.e., GF mice with bacterial reconstitution at the time of delivery), pregnant GF mice were housed with SPF female mice 1 day before delivery and only male pups were retrieved after weaning. To generate 5WexGF mice (i.e., GF mice with bacterial reconstitution at 5 weeks old), since 5 weeks, GF male mice began to cohabit with SPF female mice of the same age. Male mice in all groups were sacrificed at 9 weeks of age.Back to article page