Table 1 DNA extraction, PCR amplification and sequencing results
From: Microbial transformation from normal oral microbiota to acute endodontic infections
Sample | Systemic or Local | DNA Concentration (ng/ul) | Total Volume (ul) | Total DNA (ug) | PCR Amplification | Number of Sequencing Reads |
|---|---|---|---|---|---|---|
E007 AS | Local | 192.40 | 42.00 | 8080.80 | successful | 7581 |
E007 OS | Local | 20.50 | 33.00 | 676.50 | successful | 10927 |
E007 RC | Local | 9.20 | 12.00 | 110.40 | low PCR yield (84.21ng) | 8791 |
E009 AS | Systemic | 13.60 | 25.00 | 340.00 | no amplification | n/a |
E009 OS | Systemic | 33.60 | 32.00 | 1075.20 | successful | 11876 |
E009 RC | Systemic | 46.40 | 37.00 | 1716.80 | successful | 11319 |
E010 AS | Systemic | 1561.80 | 36.00 | 56224.80 | low PCR yield (61.08ng) | 4277 |
E010 OS | Systemic | 126.60 | 34.00 | 4304.40 | successful | 9435 |
E010 RC | Systemic | 21.00 | 35.00 | 735.00 | successful | 11504 |
E012 AS | Local | 4503.20 | 42.00 | 189134.40 | low PCR yield (93.39ng) | 5800 |
E012 OS | Local | 394.70 | 35.00 | 13814.50 | successful | 11211 |
E012 RC | Local | 67.30 | 40.00 | 2692.00 | successful | 11835 |
E016 AS | Local | 15.50 | 27.00 | 418.50 | low PCR yield (80.46ng) | 6774 |
E016 OS | Local | 19.60 | 27.00 | 529.20 | successful | 12651 |
E016 RC | Local | 6.50 | 5.00 | 32.50 | successful | 11809 |
E017 AS | Local | 199.90 | 40.00 | 7996.00 | successful | 10735 |
E017 OS | Local | 22.00 | 35.00 | 770.00 | successful | 11305 |
E017 RC | Local | 128.20 | 40.00 | 5128.00 | successful | 10365 |
E018 AS | Systemic | 727.20 | 40.00 | 29088.00 | successful | 11439 |
E018 OS | Systemic | 15.80 | 30.00 | 474.00 | successful | 10148 |
E018 RC | Systemic | 23.50 | 31.00 | 728.50 | successful | 8601 |
E019 AS | Local | 18.30 | 30.00 | 549.00 | successful | 16937 |
E019 OS | Local | 16.50 | 27.00 | 445.50 | successful | 6575 |
E019 RC | Local | 734.10 | 35.00 | 25693.50 | successful | 9624 |