Figure 8

Assessment of error and bias using increasingly divergent genomic reference species as proxies. a) Number of genes in different datasets derived using either H. sapiens, or each of the six species evolutionarily divergent from H. sapiens as the genomic reference. Lines are as follows: Blue square - the total number of genes in the filtered species dataset; Red triangle - the number of genes that have TA contig hits; Green circle - Number of genes with CRR > = 90%; b) Blue diamond - Comparison of the Spearman’s correlation (ρ) for expression values obtained through annotating TA contigs using the CCDS dataset and using the proxy GRS datasets; Red squares - Level of error incurred through using divergent GRS to annotate TA measured as the percentage of TA contigs incorrectly assigned to CCDS; c) Bias obtained through using GRS as proxy datasets (number of GO and/or KEGG categories): Red triangles - GRS genes orthologous to human CCDS genes; Blue squares - subset of the GRS orthologs that have only TA contigs that are correctly assigned to them; Green circles - residuals from a graph of expression values obtained via mapping to the TA and then annotated either directly to the CCDS or to a GRS gene set. Significance is at p < 0.05 in all cases; d) Approximate divergence times of proxy GRS from H. sapiens (taken from [30–33].