Figure 2
Expression analysis of the subtilisin-like serine protease inhibitor gene Ta.22614.1.S1_at in two cultivar pairs. Ta.22614.1.S1_at was analysed as a candidate gene for resistance against fungal-derived proteases. The qPCR time-course experiment was conducted on infected spikes of (A) the moderately FHB-resistant cv. Dream and the susceptible cv. Lynx; and (B) the FHB-resistant cv. Sumai 3 and the susceptible cv. Florence-Aurore. Fold increases were calculated relative to the internal standard gene (ubiquitin) and a water treated control sample (mock) of the respective sampling time using the comparative Ct-Method. Columns represent average induction (+SE, n = 3) plotted on a 2x logarithmic scale. Hash symbols indicate measurements where no gene expression was observed.