Skip to main content

Table 1 Statistics of sRNA sequences from B. napus

From: Identification of miRNAs and their targets from Brassica napus by high-throughput sequencing and degradome analysis

 

Redundant

Non-redundant

 

Number of counts

% of total

Number of unique sequence

% of total

Raw reads

13020106

\

2149116

\

Adaptor removed

30673

0.24

25713

1.2

Junk Filter a

5182

0.04

2362

0.11

Length filter

1379794

10.6

646357

30.08

Simple sequence filter b

69175

0.53

11859

0.55

Copy number <3

1219472

9.37

1130389

52.6

Hit mRNA, RFam, Repbase

9877816

75.87

57637

2.68

Mappable

437994

3.36

30400

1.41

  1. a the sequences are filtered out if they contain 3 Ns (N is undetermined nucleotide) and only A and C without G and T, vice versa; b the simple sequences are filtered out if they contain 2 Ns (N is undetermined nucleotide), 7 consecutive As, 8 consecutive Cs, 6 consecutive Gs or 7 consecutive Ts and 10 repeats of any dimer, 6 repeats of any trimer, or 5 repeats of any tetramer. These numbers were from the statistics of miRbase ver16.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us