Figure 5
From: Serpins in rice: protein sequence analysis, phylogeny and gene expression during development
Basal expression of rice serpin genes. Real-time RT-PCR was performed using a LightCycler®. Relative transcript abundance is based on CP 20 = 100,000 units. Actin was used as a control. A. Expression in callus tissue. Total RNA was isolated from callus grown on callus-induction medium. B. Expression in developing seedlings. Total RNA was isolated from whole seedlings grown on solid growth medium at 2, 4, 6 and 8 d after germination. C. Expression in 2-week-old seedlings. Total RNA was isolated from whole seedlings grown on solid growth medium. D. Expression in organs of mature plants. Total RNA was isolated from leaves, roots and stems of mature plants grown in soil.