Primer | Sequence | Probe | PCR conditions |
---|---|---|---|
Hem_8081_FW | 5'-CAATATGACTACCGACCCGGTTAC-3' | Hem_8081 | - Denaturation at 95°C for 2 min |
Hem_8081_RV | 5'-GGATACATCTGCTGGGCGATATAC-3' | 162 bp | - 30 cycles: |
denaturation at 95°C for 30 sec | |||
Col_WA_FW | 5'-CGATCGTAGTAGTAAGGCAACTCC-3' | Col_WA | annealing at 60°C for 30 sec |
Col_WA_RV | 5'-GACGGTATCATGCCCATAACTG-3' | 234 bp | elongation at 72°C for 40 sec |
- Final elongation at 72°C for 7 min |