A high-density genetic map of Arachis duranensis, a diploid ancestor of cultivated peanut

  • Ervin D Nagy1,

    Affiliated with

    • Yufang Guo1,

      Affiliated with

      • Shunxue Tang1,

        Affiliated with

        • John E Bowers1,

          Affiliated with

          • Rebecca A Okashah1,

            Affiliated with

            • Christopher A Taylor1,

              Affiliated with

              • Dong Zhang1,

                Affiliated with

                • Sameer Khanal1,

                  Affiliated with

                  • Adam F Heesacker1,

                    Affiliated with

                    • Nelly Khalilian1,

                      Affiliated with

                      • Andrew D Farmer2,

                        Affiliated with

                        • Noelia Carrasquilla-Garcia3,

                          Affiliated with

                          • R Varma Penmetsa3,

                            Affiliated with

                            • Douglas Cook3,

                              Affiliated with

                              • H Thomas Stalker4,

                                Affiliated with

                                • Niels Nielsen4,

                                  Affiliated with

                                  • Peggy Ozias-Akins5Email author and

                                    Affiliated with

                                    • Steven J Knapp1

                                      Affiliated with

                                      BMC Genomics201213:469

                                      DOI: 10.1186/1471-2164-13-469

                                      Received: 22 December 2011

                                      Accepted: 30 August 2012

                                      Published: 11 September 2012

                                      Abstract

                                      Background

                                      Cultivated peanut (Arachis hypogaea) is an allotetraploid species whose ancestral genomes are most likely derived from the A-genome species, A. duranensis, and the B-genome species, A. ipaensis. The very recent (several millennia) evolutionary origin of A. hypogaea has imposed a bottleneck for allelic and phenotypic diversity within the cultigen. However, wild diploid relatives are a rich source of alleles that could be used for crop improvement and their simpler genomes can be more easily analyzed while providing insight into the structure of the allotetraploid peanut genome. The objective of this research was to establish a high-density genetic map of the diploid species A. duranensis based on de novo generated EST databases. Arachis duranensis was chosen for mapping because it is the A-genome progenitor of cultivated peanut and also in order to circumvent the confounding effects of gene duplication associated with allopolyploidy in A. hypogaea.

                                      Results

                                      More than one million expressed sequence tag (EST) sequences generated from normalized cDNA libraries of A. duranensis were assembled into 81,116 unique transcripts. Mining this dataset, 1236 EST-SNP markers were developed between two A. duranensis accessions, PI 475887 and Grif 15036. An additional 300 SNP markers also were developed from genomic sequences representing conserved legume orthologs. Of the 1536 SNP markers, 1054 were placed on a genetic map. In addition, 598 EST-SSR markers identified in A. hypogaea assemblies were included in the map along with 37 disease resistance gene candidate (RGC) and 35 other previously published markers. In total, 1724 markers spanning 1081.3 cM over 10 linkage groups were mapped. Gene sequences that provided mapped markers were annotated using similarity searches in three different databases, and gene ontology descriptions were determined using the Medicago Gene Atlas and TAIR databases. Synteny analysis between A. duranensis, Medicago and Glycine revealed significant stretches of conserved gene clusters spread across the peanut genome. A higher level of colinearity was detected between A. duranensis and Glycine than with Medicago.

                                      Conclusions

                                      The first high-density, gene-based linkage map for A. duranensis was generated that can serve as a reference map for both wild and cultivated Arachis species. The markers developed here are valuable resources for the peanut, and more broadly, to the legume research community. The A-genome map will have utility for fine mapping in other peanut species and has already had application for mapping a nematode resistance gene that was introgressed into A. hypogaea from A. cardenasii.

                                      Background

                                      Cultivated peanut (Arachis hypogaea L.) is a major crop in most tropical and subtropical areas of the world and provides a significant source of oil and protein to large segments of the population in Asia, Africa and the Americas. In the U. S., peanut is a high-value cash crop of regional importance, with major production areas concentrated in the Southeast. Plant breeding efforts to pyramid genes for disease and insect resistances, quality, and yield is hampered by the polyploid genetics of the crop species, the multigenic nature of many traits (e.g., yield), and the difficulty of selecting for many traits in the field (e.g., soil borne diseases). Thus, secondary selection methods that are environmentally neutral would greatly facilitate crop improvement efforts. Molecular markers fit this criterion, but only recently have markers been developed that reveal sufficient polymorphisms in A. hypogaea and related species to have wide-spread application in peanut breeding. Preliminary steps for utilizing molecular markers for crop improvement are developing collections of polymorphic markers and utilizing them to construct dense and high-resolution genetic maps.

                                      Constructing a high-quality genetic map depends largely upon finding one or more marker systems that can detect high levels of polymorphism between two individual parents. Unfortunately, low levels of molecular polymorphism were observed within tetraploid (2n = 4x = 40) A. hypogaea throughout the 1990s and early 2000s with the marker systems available at that time [1, 2]. However, compared with the limited numbers of polymorphic markers detected for the tetraploid, the same marker systems can uncover high levels of molecular polymorphism within and between the diploid (2n = 2x = 20) peanut species. This polymorphism led researchers to create molecular maps for Arachis. The first molecular map in peanut was constructed between the diploids A. stenosperma Krapov. and W.C. Gregoryx and A. cardenasii Krapov. and W.C. Gregory by Halward et al. [3] who used Restriction Fragment Length Polymorphisms (RFLPs) to associate 117 markers into 11 linkage groups. Additional maps were subsequently published using Randomly Amplified Polymorphic DNA (RAPD) [4] and Simple Sequence Repeats (SSRs) [5, 6]. Burow et al. [7] published the first tetraploid map in peanut based on 370 RFLP loci across 23 linkage groups by utilizing the complex interspecific cross, Florunner × 4x A. batizocoi Krapov. and W.C. Gregory (A. cardenasii × A. diogoi Hoehne)]. Another interspecific tetraploid linkage map of 298 loci and 21 linkage groups was derived from a backcross population between A. hypogaea and a synthetic amphidiploid [8]. Only recently have linkage maps been developed from crosses between A. hypogaea genotypes, most with less than 200 loci and with more than the expected 20 linkage groups [913]. An exception is the recently published map containing 1114 loci across 21 linkage groups that was constructed in part with highly polymorphic markers derived from sequences harboring miniature inverted repeat transposable elements [14]. Therefore, there is a continuing need to generate dense linkage maps for the cultivated tetraploid peanut that will not only cluster the markers into the expected 20 linkage groups to cover the haplotype chromosomes, but also to facilitate marker-trait association and eventually assist in its genetic improvement.

                                      The domesticated peanut is thought to have arisen from a single hybridization event between two diploid wild species followed by whole genome duplication approximately 3,500 years ago [15]. This short evolutionary history, along with hybridization barriers between diploids and the tetraploid have resulted in a narrow genetic base for the cultivated tetraploid peanut. On the contrary, diploid Arachis species are genetically diverse, have simpler inheritance patterns, and most importantly, contain a rich source of agronomically important traits for peanut improvement. Due to these attributes, diploid Arachis species have been proposed as model systems to map the peanut genome. Because the genomes of progenitor diploid species [i.e., A. duranensis (A-genome donor) and A. ipaensis (B-genome donor)] are closely allied to the cultivated peanut [16], mapping the genome of one or both of these species should be useful for predicting the positions of loci in the cultivated peanut. This approach has been employed in wheat [17, 18], alfalfa [19, 20], oat [21], and other crop species.

                                      One accession of A. ipaensis and 67 accessions of A. duranensis have been collected in South America. The largest concentration of A. duranensis is in southern Bolivia and northern Argentina, with a few populations being reported in Paraguay and one in central Brazil [22, 23]. The species is morphologically diverse and the Bolivia and Argentina types can be separated cytogenetically and morphologically [24]. Due to the availability of diverse accessions to produce intraspecific crosses in the greenhouse, a dense linkage map in the diploid species A. duranensis was produced using large numbers of molecular markers derived from transcribed sequences.

                                      Results and discussion

                                      Species relationships

                                      A preliminary study of SSR marker variation among 37 A. duranensis accessions using 556 markers indicated that the species is highly polymorphic at the molecular level and individual accessions could be separated based on a cluster analysis (Figure 1). Interestingly, we found that A. ipaensis, the proposed B-genome (BB) progenitor species, clustered with the A-genome (AA) species A. stenosperma and not with the B-genome species A. batizocoi. Recent molecular cytogenetic analysis of A- and non-A- (i.e., B-) genome species suggests that karyotype diversity among non-A-genome species is extensive enough to support separation into additional genome classes where A. ipaensis remains in B sensu stricto while A. batizocoi is placed into a separate group [25]. Therefore, A. batizocoi is less typical of B-genome species.
                                      http://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-469/MediaObjects/12864_2011_4559_Fig1_HTML.jpg
                                      Figure 1

                                      Genetic relationships among A- and B-genome Arachis species. Clustering of A- (A. duranensis and A. stenosperma) and B- (A. ipaensis and A. batizocoi) genome species according to analysis of data from SSR markers. The two parents used for mapping are indicated by arrows.

                                      The number of polymorphic SSR markers between paired A. duranensis accessions ranged from 160 to 375 out of 556, which is 29 to 67% of the total number of SSR markers screened. This is a significant amount of variation, which indicates the high genetic diversity within the species. Based on cluster analysis, success of crosses, and fertility of F1s, accessions PI 475887 and Grif 15036 were selected for subsequent mapping studies using 94 F2 progenies. Screening of the parental accessions with 2,138 SSR markers derived from A. hypogaea EST sequences resulted in 1,768 (82.7%) that were scorable (detected by ABI3730XL genotyping systems) and 896 (41.9%) that were polymorphic (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut, Submitted). The same markers were used to create a map between two A. batizocoi accessions and to determine syntenic relationships between the A and B genome species (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, submitted).

                                      Arachis duranensis genetic map

                                      The total number of published SSR markers has now risen beyond the 2,847 cataloged in a related paper by Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, submitted) to around 6,000 [26]. Those most recently reported include: 14 by Gimenes et al. [27]; 51 by Mace et al. [28]; 188 by Proite et al. [29]; 104 by Cuc et al. [30]; 138 by Yuan et al. [31]; 33 by Song et al. [32]; 123 by Wang et al. [33]; 290 by Liang et al. [34]; and 1,571 by Koilkonda et al. [35]. Five hundred and ninety-eight of these markers are included in the A. duranensis map (Figure 2). Of the 34 genomic SSR markers mapped in the current study (Table 1), 24 were mapped previously in an interspecific population between A. duranensis and A. stenosperma[6, 36]. These markers served to anchor and align the current and previously published peanut maps (Figure 2). Linkage group assignments of all markers were consistent between the current map and that of Bertioli et al. [36] except for the marker GM117 (AC3C02 on map in reference 36 derived from GenBank accession DQ099133) that was localized on chromosome 2A (the ‘A’ following a chromosome number is presented in this study to represent chromosomes in the A genome of peanut) in their interspecific map, while mapping to chromosome 10A in the A. duranensis intraspecific map. Although detailed information for parental alleles in the study by Bertioli et al. [36] was not presented, GM117 amplified only one locus from each parent in both their population and ours. It is, therefore, unlikely that the marker location discrepancy was due to mapping of multiple loci and perhaps could reflect a small chromosomal rearrangement. Chromosomal rearrangements are not unexpected based on previous cytological observations in the genus [24, 37].
                                      http://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-469/MediaObjects/12864_2011_4559_Fig2_HTML.jpg
                                      Figure 2

                                      High-density linkage map of Arachis duranensis including 1,724 markers. SNP and SSR markers are prefixed by ‘SNP’ and ‘GM’, respectively, resistance gene candidate markers are prefixed by ‘RGC’ and ‘GS’. Twenty-four previously published markers (underlined) were selected from an interspecific map between A. duranensis and A. stenosperma[36] to establish synteny between the current and former linkage groups. The original linkage group assignments are given in the marker names separated by the pound (#) sign. Loci with significant segregation distortion (p = 0.05) are labeled with an asterisk. Graphs to the right of the linkage groups represent recombination frequencies. Each data point represents genetic distances between adjacent markers averaged for a window of 20 markers.

                                      Table 1

                                      Previously published genomic SSR markers mapped in Arachis duranensis

                                      Universal Name

                                      Original Name

                                      Forward (5′-3′)

                                      Reverse (5′-3′)

                                      Reference

                                      GM7

                                      Ah1TC1D02

                                      GATCCAAAATCTCGCCTTGA

                                      GCTGCTCTGCACAACAAGAA

                                      Moretzsohn et al. 2005

                                      GM10

                                      Ah1TC1E05

                                      GAAGGATAAGCAATCGTCCA

                                      GGATGGGATTGAACATTTGG

                                      Moretzsohn et al. 2005

                                      GM13

                                      Ah1TC1H04

                                      CATTACTTCCTAGGTTTGTTTTCCA

                                      ATGGCGTGACAACGGAAC

                                      Moretzsohn et al. 2005

                                      GM16

                                      Ah1TC2B01

                                      TTGCAGAAAAGGCAGAGACA

                                      GAAAGAAGCTAAGAAGGACCCATA

                                      Moretzsohn et al. 2005

                                      GM19

                                      Ah1TC2C07

                                      CACCACACTCCCAAGGTTTT

                                      TCAAGAACGGCTCCAGAGTT

                                      Moretzsohn et al. 2005

                                      GM22

                                      Ah1TC2D06

                                      AGGGGGAGTCAAAGGAAAGA

                                      TCACGATCCCTTCTCCTTCA

                                      Moretzsohn et al. 2005

                                      GM24

                                      Ah1TC2E05

                                      GAATTTATAAGGCGTGGCGA

                                      CCATCCCTTCTTCCTTCACA

                                      Moretzsohn et al. 2005

                                      GM28

                                      Ah1TC3A12

                                      GCCCATATCAAGCTCCAAAA

                                      TAGCCAGCGAAGGACTCAAT

                                      Moretzsohn et al. 2005

                                      GM32

                                      Ah1TC3E02

                                      TGAAAGATAGGTTTCGGTGGA

                                      CAAACCGAAGGAGGAACTTG

                                      Moretzsohn et al. 2005

                                      GM38

                                      Ah1TC3H02

                                      CTCTCCGCCATCCATGTAAT

                                      ATGGTGAGCTCGACGCTAGT

                                      Moretzsohn et al. 2005

                                      GM58

                                      Ah1TC4G06

                                      ATTTCACATTCCCTAGCCCC

                                      CATCGACTGACTTGAAAAATGG

                                      Moretzsohn et al. 2005

                                      GM59

                                      Ah1TC4G10

                                      TTCGGTCATGTTTGTCCAGA

                                      CTCGAGTGCTCACCCTTCAT

                                      Moretzsohn et al. 2005

                                      GM66

                                      Ah1TC5D06

                                      GAAATTTTAGTTTTCAGCACAGCA

                                      TTTTCCCCTCTTAAATTTTCTCG

                                      Moretzsohn et al. 2005

                                      GM68

                                      Ah1TC6E01

                                      CTCCCTCGCTTCCTCTTTCT

                                      ACGCATTAACCACACACCAA

                                      Moretzsohn et al. 2005

                                      GM69

                                      Ah1TC6G09

                                      GGAGGTTGCATGCATCATAGT

                                      TCATTGAACGTATTTGAAAGCTC

                                      Moretzsohn et al. 2005

                                      GM71

                                      Ah2TC7A02

                                      CGAAAACGACACTATGAAACTGC

                                      CCTTGGCTTACACGACTTCCT

                                      Moretzsohn et al. 2005

                                      GM74

                                      Ah2TC7E04

                                      GAAGGACCCCATCTATTCAAA

                                      TCCGATTTCTCTCTCTCTCTCTC

                                      Moretzsohn et al. 2005

                                      GM76

                                      Ah2TC7G10

                                      AATGGGGTTCACAAGAGAGAGA

                                      CCAGCCATGCACTCATAGAATA

                                      Moretzsohn et al. 2005

                                      GM83

                                      Ah2TC9C06

                                      CAAATGGCAGAGTGCGTCTA

                                      CCCTCCTGACTGGGTCCT

                                      Moretzsohn et al. 2005

                                      GM92

                                      Ah2TC11A04

                                      ACTCTGCATGGATGGCTACAG

                                      CATGTTCGGTTTCAAGTCTCAA

                                      Moretzsohn et al. 2005

                                      GM96

                                      Ah2TC11C06

                                      TCCAACAAACCCTCTCTCTCT

                                      GAACAAGGAAGCGAAAAGAA

                                      Moretzsohn et al. 2005

                                      GM117

                                      Ah2AC3C02

                                      TCTAACGCACACAAATCGAA

                                      CTTGTACCTGCGCCATTCT

                                      Moretzsohn et al. 2005

                                      GM126

                                      AS1RI1F06

                                      TGTCTCTCTTCCTTTCCTTGCT

                                      CCTTTTGCTTCTTTGCTTCC

                                      Moretzsohn et al. 2005

                                      GM162

                                      AS1RN9C02

                                      CGTTACACTGAGCCAGCAACTC

                                      ACGGCGGCGATAGTTTCA

                                      Moretzsohn et al. 2005

                                      GM170

                                      AS1RN11E05

                                      CTCGGTCCAGAAAACACAGG

                                      GTAGAGGCGAAGAAGGCAGAG

                                      Moretzsohn et al. 2005

                                      GM218

                                      gi-30419832

                                      GCCACTTTATTCTAAGCACTCC

                                      AAGAGACCACACGCTCACA

                                      Moretzsohn et al. 2005

                                      GM226

                                      gi-30419936

                                      TCACAGATCCATAGACTTTAACATAGC

                                      CCGGTGTGGATTCATAGTAGAG

                                      Moretzsohn et al. 2005

                                      GM255

                                      pPGPSeq4H6

                                      CCAACATTGCAGAAGCAAGA

                                      CAAAGAGAGGCACACCACAA

                                      Moretzsohn et al. 2005

                                      GM286

                                      Ah-193

                                      CTTGCTGAAGGCAACTCCTACG

                                      TCGGTTTGTCTCTTTGGTCACTC

                                      Moretzsohn et al. 2004

                                      GM324

                                      Ah-649

                                      GGAAATGCCAAATCCATCCTTC

                                      GTTGTTCGGTGTGAAAACGGTC

                                      Moretzsohn et al. 2004

                                      GM328

                                      Ah-671

                                      AGAAAGAGCACGGGACATTACC

                                      ATGAATGAGTGGTCATACGCGA

                                      Moretzsohn et al. 2004

                                      GM565

                                      pPGSseq17E3

                                      TTTCCTTTCAACCCTTCGTG

                                      AATGAGACCAGCCAAAATGC

                                      Ferguson et al. 2004

                                      GM664

                                      GM664

                                      CTTCACCTCCAAAATCAACCA

                                      ACCGCTGACATTTGATTGTTC

                                      Guo et al. 2011

                                      GM671

                                      GM671

                                      TGGATGCTGTAAGGAATGGAC

                                      TTATCGAGCTTGCCTCAGAAA

                                      Guo et al. 2011

                                      Markers were renamed in order to follow a unified marker nomenclature. The complete list of renamed markers can be found in Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted).

                                      EST libraries of A. duranensis were developed to produce Single Nucleotide Polymorphism (SNP) markers for mapping (Table 2). Of the 1,536 SNP markers developed (Additional file 1), 1,054 were included in the A. duranensis map (Figure 2). The remaining 482 SNP markers were either of low quality (GC quality score <0.25) or they showed segregation patterns (extremely distorted) that could not be mapped. Of the 1,054 mapped SNP markers, 815 were derived from the cDNA sequencing project while the other 239 were genomic legume orthologs.
                                      Table 2

                                      cDNA sequence reads generated for single nucleotide polymorphism (SNP) discovery in Arachis duranensis*

                                      Accession

                                      Sequencing Platform

                                      Tissue type

                                      Total

                                        

                                      Developing seed

                                      Root

                                       

                                      PI 475887

                                      Sanger

                                      22,356

                                      21,487

                                      43,843

                                      PI 475887

                                      454

                                      212,938

                                      266,575

                                      479,513

                                      Grif 15036

                                      454

                                      296,242

                                      235,245

                                      531,487

                                      Total

                                       

                                      531,536

                                      523,307

                                      1,054,843

                                      * Assembly is deposited at NCBI as Accession: PRJNA50587.

                                      The A. duranensis map produced in this study contained 1,724 markers combined into 10 linkage groups with a total genetic distance of 1081.3 cM. MSTMap, a software program that accommodates large numbers of markers and utilizes a “minimum spanning tree” algorithm, was used to construct an initial genetic map using only the codominant markers. The 1,673 codominant markers were distributed into 810 co-segregating groups (bins). Although this program has been reported to be accurate for large-scale mapping projects [38], few independent studies are available establishing consistency between MSTMap and other commonly used mapping software [39]. To confirm the linkage group assignments, marker orders, and genetic distances determined by alternative software, both codominant and dominant markers were mapped with Joinmap 3.0. Marker orders and genetic distances were highly consistent between MSTMap and Joinmap 3.0 (Additional file 2).

                                      Significant segregation distortion (p = 0.05) was observed for 513 (29.8%) markers (Figure 2, Additional file 3). Chromosomes 4A and 9A carried particularly long segments of distorted segregation suggesting large-scale chromosomal selection in these regions. Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted) found that a single linkage group (4/9B) in A. batizocoi was syntenic with chromosomes 4A and 9A of A. duranensis implicating inversion and reciprocal translocation events as the underlying chromosomal rearrangements in this B-genome species. Recombination frequencies were generally low in the central, presumably centromeric chromosomal regions of A. duranensis and increased toward the telomeres, a pattern typical of many plant species [40, 41]. More even distribution was observed along chromosome 3A and only slightly suppressed recombination was observed around the presumable location of the centromere (Figure 2).

                                      Across the A. duranensis linkage map, each linkage group spanned on average 108.1 cM (77.3-145.6 cM) and included 172.4 markers (119–266) (Table 3). This is considerably denser than the previously published AA, BB, and AABB maps consisting of only a few hundred markers. For example, the A. ipaensis × A. magna B-genome map published by Moretzsohn et al. [5] had 149 SSR markers grouped into 10 linkages, whereas the B-genome SSR-based map in our related paper consists of 449 loci in 16 linkage groups (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted). The A-genome map produced using the interspecific hybrid A. duranensis × A. stenosperma had 339 SSRs that were mapped into 11 linkage groups [6, 42]. For A. hypogaea, there are now several maps with the most dense consensus map containing 324 loci on 21 linkage groups [11].
                                      Table 3

                                      Genetic distances and distribution of markers on the ten linkage groups of A. duranensis

                                      Linkage group

                                      Genetic distance (cM)

                                      Markers

                                      1A

                                      96.8

                                      186

                                      2A

                                      103.9

                                      119

                                      3A

                                      145.6

                                      266

                                      4A

                                      115.8

                                      149

                                      5A

                                      131.7

                                      178

                                      6A

                                      109.8

                                      181

                                      7A

                                      82.3

                                      141

                                      8A

                                      77.3

                                      180

                                      9A

                                      106.5

                                      171

                                      10A

                                      111.4

                                      153

                                      Total

                                      1081.3

                                      1724

                                      The map produced in the current study is the first high-density map available in peanut, and because it was generated from a progenitor species of A. hypogaea, we anticipate that it will have significant applications for analyzing the cultivated genome. For example, the data generated in this map was used by Nagy et al. [43] to more precisely map the Rma gene for nematode resistance that originated from an introgression line between A. hypogaea and A. cardenasii. The A-genome SNP array also has been useful at the tetraploid level for genotyping a recombinant inbred line population derived from a cross between cultivated peanut and a synthetic A. ipaensis x A. duranensis tetraploid (Ozias-Akins, unpublished).

                                      Gene annotation and comparative mapping

                                      Homology search of the 1,724 mapped loci resulted in significant hits for 1,463 loci in at least one of the three databases: Medicago, Uniprot and GenBank NR database, and 580 of the mapped loci gave significant similarities in either of the two gene ontology databases: Medicago Gene Atlas and TAIR (Additional file 4). Altogether 1,366 gene ontology terms were assigned to the 580 genes. These were distributed among the three major gene ontology categories as follows: 521 molecular functions, 534 biological processes, and 311 cellular components (Additional file 4).

                                      The sequences used to create the A. duranensis map also were compared to the genomes of two legumes where 995 loci on the A. duranensis map could be mapped to M. truncatula, and 2,711 matches could be found in G. max (with potentially two hits per mapped locus). While a majority of the dots in the synteny plots appear to be random (Figure 3), there are definite clusters of markers, and striking examples of colinearity (red arrows), especially for the comparisons to Glycine. Presumably there has been extensive single gene movement since the last common ancestors in one or both species, but many genes remain in the ancestral locations and can be detected. Overall, the synteny patterns for G. max showed the recent whole genome duplication within Glycine, with each location in peanut showing corresponding synteny at two locations in Glycine. Colinearity between Medicago and Arachis is much less conserved than between Arachis and Glycine. This could be due to extensive inversions in either genome, or more likely, due to preliminary ordering of sequences within the Medicago unfinished genome assembly. In general, the patterns showed strong synteny on the chromosomal ends in both genetic and physical distance, while the central regions of chromosomes tended to show less synteny. Presumably this could be attributed to pericentromeric heterochromatin which is known to define less recombinogenic regions where genomic rearrangements are more likely to persist [44]. Chromosome arms tend to be maintained as syntenic between Glycine and Arachis, but there is evidence that chromosome arms have been translocated in some cases so that synteny exists at the chromosome arm level, but less so at the whole chromosome level.
                                      http://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-469/MediaObjects/12864_2011_4559_Fig3_HTML.jpg
                                      Figure 3

                                      Synteny between diploid A-genome peanut ( A. duranensis , 2 n = 20) and Glycine max (2 n = 40). Arrows indicate clusters of genes in common between the two genomes. For plotting the data on the Y axis, the peanut genome for each chromosome is proportional in size to the total map size in centimorgans. For the X axis, the unit of measure is scaled to bp within the chromosomal assemblies of the respective genomes. The plot was obtained with a visual basic program that plotted the x‐y coordinates of each hit. The total number of matches for each pair wise comparison is listed at the upper left corner.

                                      Conclusions

                                      This investigation provided a large number of de novo EST sequences that were deposited into GenBank. The markers developed here are valuable resources for peanut and, more broadly to the legume research community. This research presents the first high-density molecular map in peanut with 1,724 markers grouped into the 10 expected linkage groups for an A-genome species. Because the map was produced with the progenitor species A. duranensis which contributed the A genome of A. hypogaea, it will serve as the reference map for both wild and cultivated species. Lastly, synteny was found between Arachis and the Glycine and Medicago genomes, which indicates that markers developed for other legume species may be of value for crop improvement in peanut. The A-genome map will have utility for fine mapping in other peanut species and has already had application to mapping a nematode resistance gene that was introgressed to A. hypogaea from A. cardenasii.

                                      Methods

                                      Plant materials

                                      Thirty-seven accessions of A. duranensis, 14 accessions of A. stenosperma (A genome), one accession of A. ipaensis, and eight accessions of A. batizocoi (B genome) were obtained from the USDA or NCSU germplasm collections. Plants were then grown in greenhouses at the University of Georgia at Athens. The accessions evaluated are shown in Figure 1. Hybrids were made between three pairs of A. duranensis accessions, including PI 468200 × PI 468198, PI 468319 × PI 475885, and PI 475887 × Grif 15036. The hybrid combination PI 475887 × Grif 15036 was one of the most polymorphic as revealed by using a panel of SSR markers as described below and thus was selected for subsequent mapping. PI 475887 was originally collected by Krapovickas, Schinini, and Simpson near Salta, Argentina during 1980, and Grif 15036 was originally collected by Williams, Simpson, and Vargas near Boqueron, Paraguay during 2002 [22]. Crosses were made by manually emasculating flowers of the female parent PI 475887 during the late afternoon and pollinating stigmas between 8 and 10 am the following morning with pollen from the male parent Grif 15036. An F2 population was developed by self-pollinating multiple F1 individuals. The intraspecific F2 population (n = 94) from a hybrid between two A. duranensis accessions was then used for mapping studies.

                                      Molecular diversity between and within A- and B-genome diploid species

                                      DNA was isolated from leaf samples of A. duranensis, A. ipaensis, A. stenosperma, and A. batizocoi accessions using a modified CTAB method [45, 46]. The 60 DNA samples were amplified using 709 different SSR primer pairs (GM1-GM709) that had been generated from sequences reported in the literature [6, 29, 4753] and screened for polymorphisms. SSR markers were genotyped on an ABI3730XL Capillary DNA Sequencer (Applied Biosystems, Foster City, CA) as described in a related paper by Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted) using forward primers labelled with FAM, HEX, or TAMRA fluorophores. Microsat [54] was used for construction of a distance matrix based on the proportion of shared bands (D = 1 - ps) from 556 primer pairs amplifying polymorphic fragments. The matrix was imported into Phylip v3.67 [55] for the construction of the neighbor-joining tree.

                                      Marker development

                                      Simple sequence repeat (SSR) markers

                                      A total of 101,132 unigenes (37,916 contigs (GenBank Acc. No. EZ720985-EZ758900) and 63,216 singletons) from tetraploid peanut ESTs (GenBank Acc. No. CD037499-CD038843, ES702769-ES768453, GO256999-GO269325, GO322902- GO343529 and short-read Sequence Read Archive accessions SRX020012, SRX019979, SRX019972, SRX019971) representing ca. 37 Mb of the A. hypogaea genome were mined for 2,138 EST-SSR markers (GM710-GM2847) (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted). Unigenes in the transcript assembly were screened for perfect repeat motifs using SSR-IT http://​www.​gramene.​org/​db/​markers/​ssrtool) and for imperfect motifs using FastPCR (http://​primerdigital.​com/​fastpcr.​html). The repeat count (n) threshold for each motif type was set for n ≥ 5. SSR markers were genotyped on an ABI3730XL Capillary DNA Sequencer (Applied Biosystems, Foster City, CA) using forward primers labelled with FAM, HEX, or TAMRA fluorophores. PCR was performed in a 12 μL reaction mixture containing 1.0 × PCR buffer, 2.5 mM Mg++, 0.2 mM each of dNTPs, 5.0 pmol of each primer, 0.5 unit of Taq polymerase, and 10 ng of genomic DNA. Touchdown PCR was used to reduce spurious amplification. The SSR markers were screened for length polymorphisms using GeneMapper 3.0 software (Applied Biosystems, Foster City, CA). Of the 2,138 EST-SSR primer pairs tested, markers derived from 598 could be mapped. A set of 34 SSR markers from genomic sequences of Arachis previously screened for polymorphism between parents of the A. duranensis mapping population (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted) were also mapped (Table 1).

                                      Single-stranded conformational polymorphism (SSCP) markers

                                      SSCP markers were developed from genomic DNA templates for previously described NBS sequences isolated by targeting conserved sequence motifs in NBS-LRR encoding genes [56, 57] and from Arachis unigenes showing similarity to R-gene homologs identified by mining a peanut transcript assembly [43]. SSCP fragments were amplified using touch-down PCR and detected by silver-staining as previously described [5860]. A total of 380 SSCP markers were evaluated for polymorphism between the parents PI 475887 and Grif 15036. The resistance gene analog markers are prefixed by either ‘GS’ or ‘RGC’ in the map. cDNA sequences for unigenes targeted for SSCP marker development in the present study were deposited in GenBank (Acc. No. GF100476-GF100638). One additional marker, the SCAR marker S197 linked to a root-knot nematode resistance gene in Arachis hypogaea[43, 61] was also mapped.

                                      Development of single nucleotide polymorphism (SNP) markers

                                      Total RNA was isolated from roots of young seedlings (up to four trifoliate) and from developing seeds (up to developmental stage R6) of the two parental genotypes, PI 475887 and Grif 15036 (alias DUR25 and DUR2, respectively). cDNA libraries were developed using the Mint cDNA synthesis kit (Evrogen) and normalized using the Trimmer cDNA normalization kit (Evrogen). cDNA sequences were generated by Sanger and 454 GS-FLX sequencing methods and assembled using the tool Mira [62]. Altogether, more than one million cDNA sequence reads were generated from A. duranensis PI 475887 and Grif 15036. These were assembled into 81,116 unique transcripts (unigenes) (GenBank Accn. No. HP000001-HP081116). Assemblies were searched for single nucleotide polymorphisms (SNPs) that fulfilled the following two criteria: (a) the SNP position is covered at least by two reads from each genotype, and (b) at least 80% of the reads call the SNP in the particular genotype. Using these criteria, we identified 8,478 SNPs in 3,922 unigenes. To facilitate the selection of candidate SNPs for designing and building Illumina GoldenGate SNP genotyping arrays, putative intron positions were predicted by aligning Arachis contigs with Arabidopsis and Medicago genomic DNA sequences identified by BLAST analyses. SNPs within 60 bp of a putative intron were eliminated, thereby reducing the collection of candidate SNPs to 6,789 in 3,264 unigenes from which 1,236 high-quality SNPs, each representing separate unigenes, were selected for genotyping. SNPs were also detected by allele re-sequencing in a subset of 768 conserved legume orthologs identified by coauthors (R.V. Penmetsa, N. Carrasquilla-Garcia, A. D. Farmer and D.R. Cook), and 300 of these SNPs were added to the GoldenGate array. SNP genotyping on the GoldenGate array was conducted at the Emory Biomarker Service Center, Emory University. The BeadStudio (Illumina) genotyping module was used for calling genotypes. Markers with GC quality scores lower than 0.25 were excluded from subsequent analysis.

                                      Map construction

                                      The program, MSTMap [39] was used to build a core genetic map including all codominant markers using the cut-off p-value of 10-12 for clustering markers into linkage groups. The recombinant inbred line2 (RIL2) algorithm and Kosambi function were used to calculate genetic distances. The program Joinmap 3.0 [63] was used to localize the dominant markers and to confirm the marker order, a range of LOD scores of 5–16 was used to create groups. The Kosambi mapping function was used for map length estimations. Markers were tested for segregation distortion by the chi-square test. Graphic presentation of the map was drawn using Mapchart 2.0 software [64].

                                      Gene annotation

                                      The cDNA sequences included in the genetic map have been used to search for homologous genes in the Medicago (http://​www.​medicago.​org), Uniprot (http://​www.​uniprot.​org) and GenBank NR (http://​www.​ncbi.​nlm.​nih.​gov/​genbank) databases using various blast algorithms. Gene ontology annotations were also added by searching Medicago Gene Atlas (http://​mtgea.​noble.​org) and The Arabidopsis Information Resource (TAIR, http://​www.​arabidopsis.​org) databases. A significance threshold of E =1e-5 was applied in all inquiries.

                                      Synteny between Arachis, Medicago, and Glycine

                                      The EST sequences used for marker-development were compared to the whole genome sequences of Glycine max and Medicago truncatula to establish synteny. Sequences for the genomes G. max V5 and M. truncatula MT3.0 were obtained through http://​www.​phytozome.​net. The sequences associated with each locus on the A. duranensis peanut map (Additional file 1 and Additional file 5) were searched against the respective whole genome sequences using blastn and E < =1e-6. For comparison to Medicago, only the best match was retained because diploid peanut and M. truncatula are at the same relative ploidy level. However for Glycine, the two best matches for each peanut sequence were retained because of the recent polyploidy within soybean and the high level of retention of duplicated genes in the species. Blast hits to scaffolds or Bacterial Artificial Chromosomes (BACs) not anchored to the chromosomal assembly in the target genomes were discarded. Plotting the data and processing of blast results were performed with Visual Basic programs written for this study.

                                      Declarations

                                      Acknowledgements

                                      This research was supported by funding from the USDA National Institute of Food and Agriculture National Research Initiative Competitive Grants Program (#2006-35604-17242) awarded to SJK and POA and by the National Peanut Board, the Peanut Foundation, the Georgia Seed Development Commission, and Georgia Research Alliance endowment funding awarded to SJK.

                                      Authors’ Affiliations

                                      (1)
                                      Institute of Plant Breeding, Genetics and Genomics, University of Georgia
                                      (2)
                                      National Center for Genome Resources
                                      (3)
                                      Department of Plant Pathology, University of California
                                      (4)
                                      Department of Crop Science, North Carolina State University
                                      (5)
                                      Department of Horticulture, University of Georgia

                                      References

                                      1. Stalker HT, Mozingo LG: Molecular markers of Arachis and marker-assisted selection. Peanut Sci 2001, 28:117–123.View Article
                                      2. Paterson AH, Stalker HT, Gallo-Meagher M, Burow MD, Dwivedi SL, Crouch JH, Mace ES: Genomics and genetic enhancement of peanut. In Genomics for Legume Crops. Edited by: Wilson RF, Stalker HT, Brummer CE. Amer Oil Chem Soc, Champaign, IL; 2004:97–109.
                                      3. Halward T, Stalker HT, Kochert G: Development of an RFLP linkage map in diploid peanut species. Theor Appl Genet 1993, 87:379–384.View Article
                                      4. Garcia GM, Stalker HT, Schroeder E, Lyerly JH, Kochert G: A RAPD-based linkage map of peanut based on a backcross population between the two diploid species of Arachis stenosperma and A. cardenasii. Peanut Sci 2005, 32:1–8.View Article
                                      5. Moretzsohn MC, Barbosa AVG, ves-Freitas DMT, Teixeira C, Leal-Bertioli SCM, Guimaraes PM, Pereira RW, Lopes CR, Cavallari MM, Valls JFM, et al.: A linkage map for the B-genome of Arachis (Fabaceae) and its synteny to the A-genome. BMC Plant Biol 2009, 9:40.PubMedView Article
                                      6. Moretzsohn MC, Leoi L, Proite K, Guimaraes PM, Leal-Bertioli SCM, Gimenes MA, Martins WS, Valls JFM, Grattapaglia D, Bertioli DJ: A microsatellite-based, gene-rich linkage map for the AA genome of Arachis (Fabaceae). Theor Appl Genet 2005, 111:1060–1071.PubMedView Article
                                      7. Burow MD, Simpson CE, Starr JL, Paterson AH: Transmission genetics of chromatin from a synthetic amphidiploid to cultivated peanut (Arachis hypogaea L.): Broadening the gene pool of a monophyletic polyploid species. Genetics 2001, 159:823–837.PubMed
                                      8. Fonceka D, Hodo-Abalo T, Rivallan R, Faye I, Sall MN, Ndoye O, Favero AP, Bertioli DJ, Glaszmann JC, Courtois B, et al.: Genetic mapping of wild introgressions into cultivated peanut: a way toward enlarging the genetic basis of a recent allotetraploid. BMC Plant Biol 2009, 9:103.PubMedView Article
                                      9. Varshney RK, Bertioli DJ, Moretzsohn MC, Vadez V, Krishnamurthy L, Aruna R, Nigam SN, Moss BJ, Seetha K, Ravi K, et al.: The first SSR-based genetic linkage map for cultivated groundnut (Arachis hypogaea L.). Theor Appl Genet 2009, 118:729–739.PubMedView Article
                                      10. Hong YB, Chen XP, Liang XQ, Liu HY, Zhou GY, Li SX, Wen SJ, Holbrook CC, Guo BZ: A SSR-based composite genetic linkage map for the cultivated peanut (Arachis hypogaea L.) genome. BMC Plant Biol 2010, 10:17.PubMedView Article
                                      11. Qin H, Feng S, Chen C, Guo Y, Knapp S, Culbreath A, He G, Wang M, Zhang X, Holbrook CC, Ozias-Akins P, Guo B: An integrated genetic linkage map of cultivated peanut (Arachis hypogaea L.) constructed from two RIL populations. Theor Appl Genet 2011, 124:653–664.PubMedView Article
                                      12. Gautami B, Pandey MK, Vadez V, Nigam SN, Ratnakumar P, Krishnamurthy L, Radhakrishnan T, Gowda MVC, Narasu ML, Hoisington DA, et al.: Quantitative trait locus analysis and construction of consensus genetic map for drought tolerance traits based on three recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.). Mol Breed 2011, 30:757–772.PubMedView Article
                                      13. Ravi K, Vadez V, Isobe S, Mir RR, Guo Y, Nigam SN, Gowda MVC, Radhakrishnan T, Bertioli DJ, Knapp SJ, et al.: Identification of several small main-effect QTLs and a large number of epistatic QTLs for drought tolerance related traits in groundnut (Arachis hypogaea L.). Theor Appl Genet 2011, 122:1119–1132.PubMedView Article
                                      14. Shirasawa K, Koilkonda P, Aoki K, Hirakawa H, Tabata S, Watanabe M, Hasegawa M, Kiyoshima H, Suzuki S, Kuwata C, et al.: In silico polymorphism analysis for the development of simple sequence repeat and transposon markers and construction of linkage map in cultivated peanut. BMC Plant Biol 2012, 12:80.PubMedView Article
                                      15. Singh AK, Simpson CE: Biosystematics and genetic resources, Chap.4 in the Groundnut crop: A scientific basis for improvement. Edited by: Smart J. Chapman &Hall, London; 1994.
                                      16. Kochert G, Stalker HT, Gimenes M, Galgaro L, Lopes CR, Moore K: RFLP and cytogenetic evidence on the origin and evolution of allotetraploid domesticated peanut, Arachis hypogaea (Leguminosae). Am J Bot 1996, 83:1282–1291.View Article
                                      17. Guyomarc'h H, Sourdille P, Charmet G, Edwards KJ, Bernard M: Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 2002, 104:1164–1172.PubMedView Article
                                      18. Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS: A genetic linkage map of Triticum tauschii (DD) and its relationship to the D-genome of bread wheat (AABBDD). Genome 1991, 34:362–374.View Article
                                      19. Echt CS, Kidwell KK, Knapp SJ, Osborn TC, McCoy TJ: Linkage mapping in diploid alfalfa (Medicago sativa). Genome 1993, 37:61–71.View Article
                                      20. Brummer EC, Bouton JH, Kochert G: Development of an RFLP map in diploid alfalfa. Theor Appl Genet 1993, 86:329–332.View Article
                                      21. Yu GX, Wise RP: An anchored AFLP- and retrotransposon-based map of diploid Avena. Genome 2000, 43:736–749.PubMed
                                      22. Stalker HT, Ferguson ME, Valls JFM, Pittman RN, Simpson CE, Bramel-Cox P: Catalog of Arachis germplasm collection. 2002. http://​wwwicrisatorg/​text/​research/​grep/​homepage/​groundnut/​arachis/​starthtm
                                      23. USDA-ARS: Germplasm Resources Information Network Species Records of Arachis. 2011. http://​wwwars-gringov/​cgi-bin/​npgs/​html/​splistpl?​889
                                      24. Stalker HT, Kochert GD, Dhesi JS: Genetic diversity within the species Arachis duranensis Krapov. & W.C. Gregory, a possible progenitor of cultivated peanut. Genome 1995, 38:1201–1212.PubMedView Article
                                      25. Robledo G, Seijo G: Species relationships among the wild B genome of Arachis species (section Arachis) based on FISH mapping of rDNA loci and heterochromatin detection: A new proposal for genome arrangement. Theor Appl Genet 2010, 121:1033–1046.PubMedView Article
                                      26. Pandey MK, Monyo E, Ozias-Akins P, Liang X, Guimarães P, Nigam SN, Upadhyaya HD, Janila P, Zhang X, Guo B, et al.: Advances in Arachis genomics for peanut improvement. Biotech Adv 2012, 30:639–651.View Article
                                      27. Gimenes MA, Hoshino AA, Barbosa AVG, Palmieri DA, Lopes CR: Characterization and transferability of microsatellite markers of the cultivated peanut (Arachis hypogaea). BMC Plant Biol 2007, 7:9.PubMedView Article
                                      28. Mace ES, Varshney RK, Mahalakshmi V, Seetha K, Gafoor A, Leeladevi Y, Crouch JH: In silico development of simple sequence repeat markers within the aeschynomenoid/dalbergoid and genistoid clades of the Leguminosae family and their transferability to Arachis hypogaea, groundnut. Plant Sci 2008, 174:51–60.View Article
                                      29. Proite K, Leal-Bertioli S, Bertioli D, Moretzsohn M, da Silva F, Martins N, Guimaraes P: ESTs from a wild Arachis species for gene discovery and marker development. BMC Plant Biol 2007, 7:7.PubMedView Article
                                      30. Cuc LM, Mace ES, Crouch JH, Quang VD, Long TD, Varshney RK: Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea). BMC Plant Biol 2008, 8:55.PubMedView Article
                                      31. Yuan M, Gong LM, Meng RH, Li SL, Dang P, Guo BZ, He GH: Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L.). Electronic J Biotech 2010,13(6):6.
                                      32. Song GQ, Li MJ, Xiao H, Wang XJ, Tang RH, Xia H, Zhao CZ, Bi YP: EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.). Electronic J Biotech 2010,13(3):10.
                                      33. Wang CT, Yang XD, Chen DX, Yu SL, Liu GZ, Tang YY, Xu JZ: Isolation of simple sequence repeats from groundnut. Electronic J Biotech 2007,10(3):10.
                                      34. Liang XQ, Chen XP, Hong YB, Liu HY, Zhou GY, Li SX, Guo BZ: Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L.) and Arachis wild species. BMC Plant Biol 2009, 9:35.PubMedView Article
                                      35. Koilkonda P, Sato S, Tabata S, Shirasawa K, Hirakawa H, Sakai H, Sasamoto S, Watanabe A, Wada T, Kishida Y, et al.: Large-scale development of expressed sequence tag-derived simple sequence repeat markers and diversity analysis in. Mol Breed 2012, 30:125–138.PubMedView Article
                                      36. Bertioli DJ, Moretzsohn MC, Madsen LH, Sandal N, Leal-Bertioli SCM, Guimaraes PM, Hougaard BK, Fredslund J, Schauser L, Nielsen AM, et al.: An analysis of synteny of Arachis with Lotus and Medicago sheds new light on the structure, stability and evolution of legume genomes. BMC Genomics 2009, 10:45.PubMedView Article
                                      37. Stalker HT, Dhesi JS, Parry D: An analysis of the B genome species Arachis batizocoi (Fabaceae). Plant Syst Evol 1991, 174:159–169.View Article
                                      38. Wells DE, Gutierrez L, Xu Z, Krylov V, Macha J, Blankenburg KP, Hitchens M, Bellot LJ, Spivey M, Stemple DL, et al.: A genetic map of Xenopus tropicalis. Devel Biol 2011, 354:1–8.View Article
                                      39. Wu Y, Bhat PR, Close TJ, Lonardi S: Efficient and accurate construction of genetic linkage maps from the minimum spanning tree of a graph. PLoS Genet 2008, 4:10.View Article
                                      40. Heslop-Harrison JS: Comparative genome organization in plants: From sequence and markers to chromatin and chromosomes. Plant Cell 2000, 12:617–635.PubMed
                                      41. Paape T, Zhou P, Branca A, Briskine R, Young N, Tiffin P: Fine-scale population recombination rates, hotspots, and correlates of recombination in the Medicago truncatula genome. Genome Biol Evol 2012, 4:726–732.PubMedView Article
                                      42. Leal-Bertioli SCM, Jose ACVF, ves-Freitas DMT, Moretzsohn MC, Guimaraes PM, Nielen S, Vidigal BS, Pereira RW, Pike J, Favero AP, et al.: Identification of candidate genome regions controlling disease resistance in Arachis. BMC Plant Biol 2009, 9:112.PubMedView Article
                                      43. Nagy ED, Chu Y, Guo YF, Khanal S, Tang SX, Li Y, Dong WBB, Timper P, Taylor C, Ozias-Akins P, et al.: Recombination is suppressed in an alien introgression in peanut harboring Rma, a dominant root-knot nematode resistance gene. Mol Breed 2010, 26:357–370.View Article
                                      44. Bowers JE, Arias MA, Asher R, Avise JA, Ball RT, Brewer GA, Buss RW, Chen AH, Edwards TM, Estill JC, et al.: Comparative physical mapping links conservation of microsynteny to chromosome structure and recombination in grasses. Proc Natl Acad Sci USA 2005, 102:13206–13211.PubMedView Article
                                      45. Murray MG, Thompson WR: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 1980, 8:4321–4325.PubMedView Article
                                      46. Doyle JJ, Doyle JL: A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 1987, 19:11–15.
                                      47. Hopkins MS, Casa AM, Wang T, Mitchell SE, Dean RE, Kochert GD, Kresovich S: Discovery and characterization of polymorphic simple sequence repeats (SSRs) in peanut. Crop Sci 1999, 39:1243–1247.View Article
                                      48. Palmieri DA, Bechara MD, Curi RA, Gimenes MA, Lopes CR: Novel polymorphic microsatellite markers in section Caulorrhizae (Arachis, Fabaceae). Mol Ecol Notes 2005, 5:77–79.View Article
                                      49. Palmieri DA, Hoshino AA, Bravo JP, Lopes CR, Gimenes MA: Isolation and characterization of microsatellite loci from the forage species Arachis pintoi (Genus Arachis). Mol Ecol Notes 2002, 2:551–553.View Article
                                      50. He G, Meng R, Newman M, Gao G, Pittman RN, Prakash CS: Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.). BMC Plant Biol 2003, 3:3.PubMedView Article
                                      51. Ferguson ME, Burow MD, Schulze SR, Bramel PJ, Paterson AH, Kresovich S, Mitchell S: Microsatellite identification and characterization in peanut (A. hypogaea L.). Theor Appl Genet 2004, 108:1064–1070.PubMedView Article
                                      52. Krishna GK, Zhang J, Burow M, Pittman RN, Delikostadinov SG, Lu Y, Puppala N: Genetic diversity analysis in Valencia peanut (Arachis hypogaea L.) using microsatellite markers. Cell Mol Biol Lett 2004, 9:685–697.PubMed
                                      53. Moretzsohn MC, Hopkins MS, Mitchell SE, Kresovich S, Valls JF, Ferreira ME: Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol 2004, 4:11.View Article
                                      54. Minch E, Ruiz-Linares A, Goldstein D, Feldman M, Cavalli-Sforza LL: MICROSAT: A computer program for calculating various statistics on microsatellite allele data, ver. 1.5d. 1997. http://​hpglstanfordedu/​projects/​microsat/​
                                      55. Felsenstein J: PHYLIP - Phylogeny Inference Package (version 3.2). Cladistics 1989, 5:164–166.
                                      56. Bertioli DJ, Leal-Bertioli SCM, Lion MB, Santos VL, Pappas G, Cannon SB, Guimaraes PM: A large scale analysis of resistance gene homologues in Arachis. Mol Genet Genom 2003, 270:34–45.View Article
                                      57. Yuksel B, Estill JC, Schulze SR, Paterson AH: Organization and evolution of resistance gene analogs in peanut. Mol Genet Genom 2005, 274:248–263.View Article
                                      58. Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T: Detection of polymorphisms of human DNA by gel-electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci USA 1989, 86:2766–2770.PubMedView Article
                                      59. Sanguinetti CJ, Neto ED, Simpson AJG: Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 1994, 17:914.PubMed
                                      60. Radwan O, Gandhi S, Heesacker A, Whitaker B, Taylor C, Plocik A, Kesseli R, Kozik A, Michelmore RW, Knapp SJ: Genetic diversity and genomic distribution of homologs encoding NBS-LRR disease resistance proteins in sunflower. Mol Genet Genom 2008, 280:111–125.View Article
                                      61. Chu Y, Holbrook CC, Timper P, Ozias-Akins P: Development of a PCR-based molecular marker to select for nematode resistance in peanut. Crop Sci 2007, 47:841–847.View Article
                                      62. Chevreux B, Pfisterer T, Drescher B, Driesel AJ, Muller WEG, Wetter T, Suhai S: Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs. Genome Res 2004, 14:1147–1159.PubMedView Article
                                      63. Van Ooijen JW, Voorrips RE: JoinMap 3.0 software for the calculation of genetic linkage maps. Plant Research Internation, Wageningen, the Netherlands; 2001.
                                      64. Voorrips RE: MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 2002, 93:77–78.PubMedView Article

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                                      This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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