Open Access

Identification of novel candidate genes for follicle selection in the broiler breeder ovary

  • Neil A McDerment1Email author,
  • Peter W Wilson1,
  • David Waddington1,
  • Ian C Dunn1 and
  • Paul M Hocking1
BMC Genomics201213:494

DOI: 10.1186/1471-2164-13-494

Received: 13 April 2012

Accepted: 14 September 2012

Published: 19 September 2012

Abstract

Background

Broiler breeders fed ad libitum are characterised by multiple ovulation, which leads to poor shell quality and egg production. Multiple ovulation is controlled by food restriction in commercial flocks. However, the level of food restriction raises welfare concerns, including that of severe hunger. Reducing the rate of multiple ovulation by genetic selection would facilitate progress towards developing a growth profile for optimum animal welfare.

Results

The study utilised 3 models of ovarian follicle development; laying hens fed ad libitum (experiment 2) and broiler breeders fed ad libitum or a restricted diet (experiments 1 & 3). This allowed us to investigate gene candidates for follicular development by comparing normal, abnormal and “controlled” follicle hierarchies at different stages of development. Several candidate genes for multiple ovulation were identified by combining microarray analysis of restricted vs. ad libitum feeding, literature searches and QPCR expression profiling throughout follicle development. Three candidate genes were confirmed by QPCR as showing significant differential expression between restricted and ad libitum feeding: FSHR, GDF9 and PDGFRL. PDGFRL, a candidate for steroidogenesis, showed significantly up-regulated expression in 6–8 mm follicles of ad libitum fed broiler breeders (P = 0.016), the period at which follicle recruitment occurs.

Conclusions

Gene candidates have been identified and evidence provided to support a possible role in regulation of ovarian function and follicle number. Further characterisation of these genes will be required to assess their potential for inclusion into breeding programmes to improve the regulation of follicle selection and reduce the need for feed restriction.

Keywords

Broiler breeder Multiple ovulation Follicle development Ovary Microarray

Background

Sexually mature hens are capable of ovulating almost once per day for the duration of their period of lay. However, although modern breeding programmes consider many more traits[1], in early breeding programmes, commercial broiler breeders that were selected primarily for rapid growth and high meat yield produced fewer eggs[2, 3]. The result of that selection pressure is that when fed ad libitum, broiler breeders produce multiple ovulations due to the development of a numerically large ovarian hierarchy[4]. While many ova from multiple ovulations are lost into the body cavity, some result in the production of double- or multiple-yolked eggs, or eggs with defective shells[3]. Additionally ad-libitum fed broiler breeders will become very heavy and develop various weight-related problems. Consequently, although juvenile broiler breeders are fed ad libitum, they are required to be limited to at least 40% of their natural nutritional intake as they reach sexual maturity to alleviate these problems[5]. However, this leaves the birds in a permanently hungry state. Feed restriction does however reduce follicle numbers in the ovarian hierarchy to a normal level[5], and consequently decreases the chance of multiple ovulation. Despite the benefits to the health of the birds, the degree of hunger experienced by the birds has raised concerns in some quarters as to the resulting impact on the birds’ welfare[3, 6, 7].

The mechanism by which feed restriction regulates follicle number has not been completely elucidated, though it is known to influence Gonadotrophin Releasing Hormone (GnRH) secretion within the hypothalamo-pituitary-gonadal (HPG) axis[8, 9]. It is likely that food restriction exploits a natural mechanism. In wild birds, clutch size is affected by availability of food and the energy required to obtain it[10]. It is clear that food intake and related growth can influence follicular selection in the ovary of birds and the result of selection for these traits has affected the regulatory mechanisms that govern follicle selection within the ovary[11, 12]. Modifying existing breeding programmes to select against multiple ovulation in commercial flocks is a potential route for reducing the requirement for feed restriction of broiler breeders.

Despite obvious differences in post-ovulatory mechanisms and specific hormone role reversals, the chicken ovulatory cycle is generally comparable with that of mammalian species[1315]. The follicle is comprised of a series of membranes and highly vascularised cell layers that surround, support, and protect the oocyte[16]. Follicles within the avian ovary are ordered progressively by size from many follicles <1 mm in diameter to the pre-ovulatory or F1 follicle at around 40 mm[17]. Follicles can be grouped into 2 basic types, white follicles, which range from 1–7 mm in diameter, and yellow follicles, from approximately 8–40 mm, which are distinguishable by the deposition of yolk, that gives them their colour, and the increased vascularisation needed to support their growth[17]. Most follicles will not grow sufficiently to undergo ovulation and the majority <8 mm will become atretic. Normally under appropriate endocrine conditions following ovulation a follicle from the 6–8 mm pool is recruited into the pre-ovulatory hierarchy[18, 19]. Once recruited and unless gonadotrophin support is removed these follicles are highly likely to mature and ovulate[17]. In pre-hierarchical development, Follicle Stimulating Hormone Receptor (FSHR) is the predominant gonadotrophin receptor[20]. However, once follicles are drafted into the hierarchy, this predominance shifts towards the Luteinising Hormone Receptor (LHR)[20].

Atresia in mammals predominantly occurs immediately prior to follicle recruitment[14, 15, 21, 22]. Studies have shown that this occurs even in the absence of the pituitary-suggesting that regulation of pre-recruitment development is intra-ovarian once it has been initiated[14, 15]. Pre-hierarchical development would therefore constitute the ‘normal’ lifespan of follicles, with progression to the hierarchy and subsequent ovulation being triggered externally[14, 15, 23].

External regulation of the avian ovary is managed through the HPG axis, with input signals being generated in the hypothalamus and relayed to the pituitary where hormone secretion is initiated[24]. The hypothalamus co-ordinates reproductive development and activity in response both to physiological and environmental signals, such as ovarian steroids[24] and photoperiod[25]. GnRH is released in pulses into the pituitary and the frequency and strength of these pulses modulate reproductive development[24] by inducing the production and release of LH and FSH from the pituitary[17, 20, 2630]. While gonadotrophin signalling leads to cell growth and proliferation, as well as intrafollicular steroidogenesis and ovulation[13, 17, 29, 3133], mapping of the downstream pathways is less complete. Due to difficulties in isolating FSH from LH, avian FSH has proved challenging to study, and is not as well understood as the mechanisms involved in LH regulation[24]. Work has been carried out to investigate the roles of Bone Morphogenetic Proteins (BMPs) and Transforming Growth Factor B (TGFB) superfamily members in intrafollicular signalling, both in early and post-recruitment development[17] but less so in the intervening stages, notably the period of follicle selection and recruitment to the hierarchy.

The aim of this study was to investigate intraovarian regulation of follicle number in chickens by identifying gene candidates which have a potential role in either intrafollicular signalling or feedback mechanisms that affect the HPG axis. Ultimately this information will increase our understanding of the mechanisms by which dysfunction in broiler breeder hen ovaries occurs and may lead to genetic or alternative strategies to reduce dependence on food restriction.

Methods

Strategy

The research utilised comparisons of ovarian function between 3 sets of animals; 1) a broiler breeder line that was feed restricted (FR) or 2) fed ad libitum (AL) and 3) a line of layer hens fed ad libitum. The feed restricted broiler breeder and ad libitum layer share a comparable ovarian hierarchy. The 3 groups were used to examine changes in gene expression between key stages in follicle development in 3 experiments;

Experiment 1, gene expression in FR vs. AL broiler breeders was compared using microarray analysis of key stages of follicle development to determine the differences between birds with a low rate of follicle selection and birds with a high rate of follicle selection. Subsequent analysis of gene expression between these stages was carried out to identify changes as follicles progressed towards and through recruitment to the hierarchy. Two analytical approaches, in R[34] and BioLayout Express[35], were used to identify significant differences within these two comparisons.

Experiment 2, laying hens, having normal follicle hierarchies, were used to screen candidate genes from experiment 1 for changes in expression in a more detailed set of follicular stages. It was reasoned that genes showing large changes around the stage associated with follicle selection would be the most likely to be involved in recruitment.

Experiment 2, laying hens, having normal follicle hierarchies, were used to screen candidate genes from experiment 1 for changes in expression in a more detailed set of follicular stages. It was reasoned that genes showing large changes around the stage associated with follicle selection would be the most likely to be involved in recruitment.

Birds and sampling: broiler breeders

Female Ross 308 broiler breeder chicks (n = 16) were reared for experiment 1 following management manual guidelines[36] with photoperiod rising to 16 L:8D by 25 weeks of age. At 29 weeks of age half the birds were allowed ad libitum access to feed and all were killed 2 weeks later. Sample collection was staggered over 2 days and was carried out 11 to 16 h after dusk. Birds were selected from a larger population at post-mortem to represent extreme ovarian phenotypes as regards numbers of hierarchical follicles. All birds had eggs present in the oviduct at sampling. At post mortem bird weight and the numbers of follicles greater >8 mm and between 5–8 mm diameter were recorded (Table1). Tissues taken for probing the microarray were the F1 follicle, 5–6 and 6–8 mm follicles and the ovarian anterior stroma. 5–6 and 6–8 mm follicles were chosen as it is at this stage that the key changes are believed to occur[18, 37]. Whole follicles were taken with yolks removed from hierarchical follicles. Female Ross 308 broiler breeder chicks (n = 23, 12 AL, 11 FR) were raised and sampled under the same conditions as above for experiment 3, with the additional inclusion of the smallest hierarchical follicle amongst the tissues taken.
Table 1

Trait means in broiler breeders from experiment 1

Variable

    Restricted

    Ad-libitum

P-value

Mean

SEM

Mean

SEM

Body Weight (Kg)

2.89

±0.11

3.79

±0.07

<0.001

Number of Follicles >8 mm

5.62

±0.18

10.25

±0.37

<0.001

Number of Follicles 5–8 mm

10.6

±1.5

12.2

±1.4

NS

Ovarian Stroma Weight (g)

4.55

±0.31

6.91

±0.38

<0.001

Birds and sampling: layers

Mature ad libitum fed White Leghorn layers (n = 8) were kept on a 28 h photoperiodic cycle (14 L:14D) for 3 weeks to synchronise ovulatory cycles. Sample collection was staggered over 3 days to allow all birds to be sampled approximately 20 h after dusk. All birds had eggs present in the oviduct at sampling. Follicles of each sample category were recorded (Table2). Sampled tissues were the anterior stroma, pre-hierarchical follicles of diameter 1–4 mm, 4–5 mm increasing in 1 mm increments to 8 mm and the F6-F1 hierarchical follicles. Whole follicles were taken with yolks removed from hierarchical follicles.
Table 2

Trait means in White Leghorn layers from experiment 2

Variable

Mean

SEM

Body Weight (Kg)

2.05

±0.10

Number of Follicles >8 mm

6.00

±0.38

Number of Follicles 5–8 mm

12.13

±2.22

Ovarian Stroma Weight (g)

5.68

±0.10

ARRIVE guidelines

Experiments were conducted after local ethical review and subsequent approval by the UK Government Home Office (Project licences 60/2926 and 60/3964) in accordance with the Animal (Scientific Procedures) Act 1986.

RNA purification

Total RNA was isolated using Ultraspec II RNA kit (AMS Bioscience, Abingdon, UK) and the quality checked using an Agilent bioanalyzer (Agilent Technologies UK, Stockport, UK) for the RNA required for the microarray analysis and on a nanodrop (Thermo Scientific, Wilmington, DE, USA) for the samples to be used for QPCR.

Microarray setup (experiment 1)

Broiler breeder samples were hybridised in 8 randomised pairs (FR v AL) for each tissue in a dye-swap microarray design using Cy3 and Cy5 (GE Healthcare, Little Chalfont, UK) dye labelled RNA. The chicken oligo microarray used was produced by ARK genomics (http://​www.​ark-genomics.​org) and contained 17 K features. Labelling was performed using a Stratagene Fairplay kit (Agilent Technologies Ltd, Stockport, UK) and hybridised using an automated GeneTAC hybridisation station (Genomic Solutions (Digilab), Huntingdon, UK).

Preparation of cDNA

For confirmatory real-time quantitative PCR (QPCR) 1 μg of total RNA was reverse transcribed using a First Strand Synthesis Kit (GE Healthcare, Little Chalfont, UK) for Experiment 2. Experiment 3 used the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems (Life Technologies), Paisley, UK). Primers for all candidates were designed using the Primer3 program[38]. All primer pairs were designed to produce products of between 150 bp and 250 bp and to be intron-spanning. All primer pairs were tested using both standard FastStart and SybrGreen PCR reagents and conditions. Production of single products was confirmed by both gel electrophoresis and by examining the dissociation curve and PCR products were confirmed by sequencing.

QPCR expression profiling (experiment 2)

Candidate genes were initially screened to determine which showed greatest variation in expression between stages of follicle development using two 4-bird pools of anterior stroma, 5–6 mm, 6–8 mm, and F4 material taken from layers. Greatest variation between consecutive tissues was estimated using the following formula (assuming an efficiency >80%).
2.5 40 - C t 2 - 40 - C t 1 10 https://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-494/MediaObjects/12864_2012_Article_4406_Equ1_HTML.gif
(1)

All primer pairs were subsequently shown to operate at estimated efficiencies of between 94-112%. This was done to prioritise candidates for comprehensive profiling. All sampled tissues were used for comprehensive profiling: QPCR for each candidate was run across 2 plates (4 birds per plate) and each plate was replicated. Lamin B Receptor (LBr) values were used for normalisation. Primers are listed in Additional file1: Table S1. QPCR was carried out on cDNA according to a Platinum Sybr green (Invitrogen) protocol with duplicates using a standard curve on an MX3000 Sequence Detection System (Stratagene). Controls (no template) were run for all primer pairs.

QPCR validation of dietary effect (experiment 3)

Bird pairs (1 AL, 1 FR) were randomised over 4 plates. QPCR was carried out on cDNA as in Experiment 2 with a positive control sample run in triplicate across plates to normalise between plates. LBr values were used for normalisation. Primers are listed in Additional file1: Table S1.

Between-treatment statistical analysis (experiment 1)

All basic statistical analysis of microarray data was carried out in an R environment[34] using the Bioconductor Limma package[39] and the protocol outlined in[40]. The data was quantile-normalised and means were calculated for replicate spots. A split-plot ANOVA was used to estimate the between-treatment effect. A Mann-Whitney non-parametric t-test was used to validate the normalisation process. All microarray analysis was corrected for multiple testing using correction for False Discovery Rate (FDR)[41].

Between-tissue statistical analysis (experiment 1)

For each probe, mean values were calculated for each bird-pair within the microarray to remove the dietary variable prior to between-tissue comparison. The datasets for the individual ovarian tissues were combined and then quantile-normalised within R prior to performing a Kruskal-Wallis one-way ANOVA to identify probes that showed significant differences between tissues. A threshold of P <0.01 was used.

Between-tissue cluster analysis (experiment 1)

An expression file was created using normalised bird-pair mean intensity values from R. This consisted of annotation columns and 32 data columns representing the 4 ovarian tissues from the 8 bird pairs. BioLayout Express3D (http://​www.​biolayout.​org/​) was used to analyse this data file. File construction and data analysis were carried out according to the protocol available from the website[35]. A Pearson correlation threshold of 0.9 was used in the initial analysis and the embedded clustering algorithm (MCL) was used to cluster genes by expression profile. Clusters were limited to n ≥ 3 where n = no. of probes.

Candidate selection (experiment 1)

Candidates identified from the comparison of follicular tissues from the microarray were selected using a multi-level filtering system. This used, as the basis, genes that were shown to be significantly differentially expressed between 2 or more follicular stages in R, and also conformed to patterns of expression (expression profiles) within BioLayout that were considered as consistent with a role in follicle recruitment. In addition, probes identified within both the Between-Treatment Statistical Analysis and the Between-Tissue Cluster Analysis in BioLayout Express were examined for supporting literature. Genes within follicle number QTL regions on chromosome 13 and the short arm of chromosome 4 [unpublished results of a low power genome scan] were also examined and those showing altered expression between ad libitum and restricted feeding in BioLayout Express consistently across bird pairs in at least 1 tissue (change in intensity >2000 units), with relevant supporting literature, were also included. A co-expression analysis was also carried out in Biolayout Express to identify genes that clustered with FSH Receptor (FSHR).

Between-tissue statistical analysis (experiment 2)

QPCR datasets for each candidate gene were log-transformed using natural logarithms. An Analysis of Variance was run in GenStat[42], using the model containing fixed effects for Tissue within Bird and Plate as a Blocking factor.

Treatment by tissue statistical analysis (experiment 3)

Following correction for plate effect and normalisation, replicate means were calculated and log-transformed. A Linear Mixed Model (REML) was run in GenStat[42] using a model with effects for Tissue x Treatment with Bird as the Block effect.

Results

Experiment 1: microarray examination of effects of release from feed restriction

Release from feed restriction to ad libitum feeding for 2 weeks increased the body weight of hens, the number of yellow yolky follicles (>8 mm), and ovarian stroma weight (Table1). Although many genes showed altered expression as a result of release from feed restriction in ovarian tissues, the level of statistical significance did not justify further investigation on this evidence alone.

Experiment 1: between-tissue analysis

Statistical analysis of the data on a between-tissue basis, after removing the dietary effect, produced 5571 probes with P-value of <0.01 and an additional 1149 at <0.05, indicating a significant difference between one or more of the tissues.

An initial network of 5189 probes was produced from the BioLayout Express analysis (Figure1). MCL produced 260 clusters of probes, 101 of which exhibited expression profiles indicative of a possible role in follicle development due to the changes in expression between tissue stages. Within the latter, 4 distinct profile types could be observed, as represented in Figure2. No probe in the profile lists from BioLayout Express had a Kruskal-Wallis (K-W) P value of >0.002 which suggests that BioLayout Express is successfully filtering out the vast majority of non-significant data at point of entry. With this double-filtering by the Kruskal-Wallis P-value and the BioLayout Express Pearson correlation, the number of probes under consideration was reduced to 1,227. The Kruskal-Wallis/BioLayout-filtered probe lists were compared with the Top-50 probes (by P value) from the feed restriction vs. ad libitum feeding comparison within tissues in order to identify any genes common to both analyses. This process identified 13 candidate genes (Table3). No apparently common related function for these genes could be identified through literature mining, although a number have functions of potential relevance to different aspects of follicular development. Genes located within the two QTL regions for follicle number were also identified and those with potentially differential expression between ad libitum and feed restricted diets, or that had a documented function of interest, were also included in the list of candidates (Table4).
https://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-494/MediaObjects/12864_2012_Article_4406_Fig1_HTML.jpg
Figure 1

3D Network of probes derived from the between-tissue expression file as visualised in BioLayout Express3D. Nodes within the network represent probes from the array. Probes are clustered based on Pearson correlations calculated in BioLayout Express. General locations of selected expression profile types (as seen in Figure2) are indicated.

https://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-494/MediaObjects/12864_2012_Article_4406_Fig2_HTML.jpg
Figure 2

The four cluster expression profiles from BioLayout Express relevant to follicle selection. Individual cluster expression profiles illustrating the patterns of expression which show changes relevant to the critical time of follicle selection. Each plot represents the mean expression at the different stages of follicular development for all probes within a single cluster representing each profile type. Each profile type was exhibited by several clusters. Numbers of probes refer to total probes exhibiting each profile type within the dataset that were also significant according to the Kruskal-Wallis Test carried out in R.

Table 3

Genes identified from comparison of the results from experiments 1 and 2

FR vs. AL Microarray Gene List

5-6 mm Top 50

6-8 mm Top 50

F1 Top 50

 Stroma Top 50

 

BioLayout Profile Gene List

Up in 5–7 mm

Up in 5–7 mm

Up in 5–7 mm

Up in 5–7 mm

Down in 5–7 mm

Genes common to both lists

MYO1C

MYO1C

GRP

RIGG01740

POSTN

YAP1

GULP1

ZNF593

SPTY2D1

PDGFRL

 

RIGG03908

MAMDC2

 

TBC1D13

 

RIGG05331

   
Table 4

Genes within follicle number QTL on chromosomes 4 and 13 where BioLayout analysis predicted up-regulation in response to ad libitum feeding

Symbol

ARKClone ID

K-W P Value

Known Function (EntrezGene)

ADRA1B

RIGG07717

0.002

Multicellular Organism Development & Cell Growth

CAMK2A

RIGG08243

NS

Ca2+ Signalling & Cell Cycle

FGF13

RIGG08380

0.003

Embryonic Development & Cell Growth

FOXI1

RIGG10898

NS

Multicellular Organism Development

GDF9

RIGG13716

<0.001

Folliculogenesis

PAK3

RIGG08561

0.012

Multicellular Organism Development

PPARGC1B

RIGG10412

NS

Oestrogen Receptor Binding

SLIT3

RIGG06586

NS

Extracellular pro-apoptotic signalling

VDAC1

RIGG13395

<0.001

Ca2+ Signalling & Regulation of Apoptosis

Subsequent co-expression analysis of the BioLayout Express dataset identified 1 gene of possible interest. Motile sperm domain containing 1 (MOSPD1) was clustered with FSHR in follicular tissues.

Experiment 2: investigation of candidates by QPCR in layers

Table2 shows trait means for the White Leghorns used in experiment 2 for expression profiling of candidate genes selected from experiment 1. It is clear from these results that broiler breeders under restricted feeding (from Table1) have a similar follicular hierarchy to that of layers.

Of an initial 60 genes under consideration on the basis of their expression pattern in BioLayout Express, 37 were confirmed as being of potential interest, including 10 of the 13 differentially expressed genes identified by cross-referencing results from the comparison of tissues and feed regime. Added to this list were 22 literature-sourced genes, 3 of which (FSHR, SMAD3 and TGFBR1) were carried through to experiment 3.

Genes with the greatest estimated fold change in the initial screen were ranked by the breadth of supporting evidence from the available literature, their BioLayout profile and experimental evidence. The 12 top ranking genes were selected as primary candidates for detailed QPCR analysis. Table5 summarises this information while the QPCR-derived expression profiles for each assay are displayed in Figures3 and4, and Additional file2: Figure S1. All 12 genes showed significant differential expression between tissues (P ≤ 0.017).
Table 5

Summary of supporting evidence for primary candidates examined in experiment 2

Gene

QPCR P value

BioLayout Profile

QTL Chromosome

AL vs. FR (Predicted)

Literature

FSHR

0.001

   

Key mediator of reproductive signalling

TGFBR1

<0.001

   

Key mediator of cell growth + survival

SMAD3

<0.001

   

Promotes Cell Survival

SLIT3

0.001

 

Gga13

Up

Promotes Apoptosis

PDGFRL

<0.001

Down 5–8 mm

 

Up

Homologs involved in steroidogenesis

VDAC1

<0.001

Up in 5–8 mm

Gga13

Up

Central to pro-apoptotic signalling

YAP1

<0.001

Up in 5–8 mm

 

Up

Possible pro-cell survival signalling

MOSPD1

<0.001

Clustered with FSHR

  

Up-regulated in ovarian cancer

KRT75

<0.001

Down 5–8 mm

  

Possible Lipid Transport

SPTY2D1

<0.001

Up in 5–8 mm

 

Down

Unknown

RIGG1740

0.017

Up in 5–8 mm

 

Down

Unknown

GULP1

<0.001

Up in 5–8 mm

 

Up

Phagocytosis of apoptotic cells

https://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-494/MediaObjects/12864_2012_Article_4406_Fig3_HTML.jpg
Figure 3

QPCR expression profiles of primary candidate genes in layers (n = 8) from experiment 2. Primary candidate status was determined at the conclusion of experiment 3. Values represent moles/mole of LBr. Note differences in the scale of the Y axis.

https://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-494/MediaObjects/12864_2012_Article_4406_Fig4_HTML.jpg
Figure 4

QPCR expression profiles in layers (n = 8) for secondary candidate genes from experiment 2. These genes were included for their documented function (Table5) and because they demonstrated the greatest changes in expression during the initial screen in experiment 2. Values represent moles/mole of LBr. Note differences in the scale of the Y axis.

Experiment 3: validation of effect of AL vs. FR on gene expression in broiler breeders

Based on the results generated by experiments 1 and 2, several literature- and microarray-sourced candidates were selected for QPCR investigation of effect of ad libitum vs. restricted feeding. While all but two candidates showed significant between-tissue effects (P ≤0.027), none demonstrated a dietary effect that was significant across tissues. Three candidates, however, demonstrated significant dietary effects within specific tissues. Both literature-sourced candidates were down-regulated under ad libitum conditions, FSHR in the F1 Follicle (P = 0.018) and GDF9 in the Anterior Stroma and in 6-8 mm follicles (P = 0.005). PDGFRL, the novel candidate identified from the microarray was shown to be upregulated in response to ad libitum feeding in 6-8 mm follicles (P = 0.016). Expression profiles for these 3 genes can be seen in Figure5. A summary of all significant effects can be found in Table6.
https://static-content.springer.com/image/art%3A10.1186%2F1471-2164-13-494/MediaObjects/12864_2012_Article_4406_Fig5_HTML.jpg
Figure 5

QPCR expression profiles of primary candidates in broiler breeders (n = 23) from experiment 3. These genes each showed significant differential expression between ad libitum and feed restricted broiler breeders. Details are summarised in Table6.

Table 6

Summary of effects identified from investigation of dietary effect in broiler breeders (experiment 3)

Gene

Candidate Source

TissueP value

TreatmentP value

InteractionP value

Interaction Effect

Tissue

GDF9

Literature/QTL

<0.001

NS

0.005

Down in Ad lib

Stroma & 6–8 mm Follicles

FSHR

Literature

<0.001

NS

0.018

Down in Ad lib

F1 Follicle

PDGFRL

Microarray

<0.001

NS

0.016

Up in Ad lib

6-8 mm Follicles

SLIT3

QTL/Microarray

<0.001

NS

NS

  

SMAD3

Literature

<0.001

NS

NS

  

BMPR2

Literature

<0.001

NS

NS

  

TGFBR1

Literature

0.005

NS

NS

  

YAP1

Microarray

0.027

NS

NS

  

VDAC1

QTL/Microarray

NS

NS

NS

  

MOSPD1

Microarray

NS

NS

NS

  

Discussion

This research was based on the premise that, as broilers and layers have been genetically selected for different traits, the fact that adult broilers exhibit contrasting ovarian phenotypes under non-restricted conditions from layers is a result of genetic selection and there is ample evidence that this is the case[5]. Consequently, the effect of this genetic selection on expression of genes suspected of being important for reproductive development was undertaken, as regulation of their expression is likely to be the cause, directly or indirectly, of the phenotypic differences observed in broilers and layers. These are obvious targets for research to explain these differences but are also of fundamental interest in reproductive biology in a wider context. The study therefore used results from a range of experimental and analytical approaches to identify candidate genes for regulation of follicle selection and recruitment in the broiler breeder ovary. Two different approaches were used in complementary fashion to analyse the large dataset produced by the microarray. BioLayout Express, the more novel component of our analytical approach, has many built-in features that complement its use as a basic analytical tool. The most fundamental is the ability to visualise the data as a 3D network of datapoints that can be subsequently analysed by the internal clustering algorithm. The results can also be filtered by any available annotation or expression profile across samples to facilitate identification of possible associations between them. This makes it much easier to ask different questions of a single dataset and was an essential asset in switching focus between developmental stages and effect of nutrition. Using these different approaches to analyse the microarray data, as well as using information from different patterns of follicular development a number of genes that show changes in expression that would be consistent with a role in follicle selection have been identified. Three of these candidates have also been shown to exhibit altered expression levels as a result of ad libitum feeding.

From the 37 prioritised candidates considered for multi-level QPCR profiling, 10 candidates (including FSHR and TGFBR1 for validation of the approach) had sufficient experimental and/or literature-sourced evidence for basic hypothesis generation as to their role in follicle selection and recruitment. All of these candidates are associated with regulation of apoptosis[4348], cell growth[20, 49, 50] and survival[51] or steroidogenesis[52] where there is any documented function. While these processes are all prerequisite to follicle survival, there is insufficient information at present to create a single model system incorporating all of our candidates, although FSHR, TGFBR1 and SMAD3, with the added possibility of MOSPD1, do interact with common signalling pathways. However, PDGFRL is by far the most intriguing novel candidate.

PDGFRL produces a product which is homologous with the functional domains of Platelet-derived Growth Factor Receptors that are involved in intrafollicular cell signalling associated with steroidogenesis in mice[52]. QPCR profiling in layers clearly shows significant (P <0.001) and substantial peaks in expression at the 5–6 mm and F2 stages, i.e. immediately prior to selection and ovulation respectively. This evidence would support a function in regulatory feedback mechanisms. The BioLayout Express profile from the broiler breeder microarray data suggests that the gene is downregulated in 5–7 mm follicles relative to the stroma and F1. This is consistent with the broiler breeder QPCR expression pattern for feed restricted birds. In contrast, the expression levels across tissues in ad libitum fed birds remain relatively constant. In ad libitum fed birds, where hierarchical follicle number is increased, the upregulation of PDGFRL expression, relative to feed restricted birds, suggests that it is likely to be in activation or upregulation of positive feedback signalling to the HPG axis. The observed downregulation in 5–7 mm follicles from broiler breeders is in marked contrast to the layers, which would support the hypothesis of potential dysregulation of part of the steroid-based feedback mechanisms, given what is already known of the PDGFR family in other species.

The QPCR results from experiment 2 show that FSHR and TGFBR1, genes known to be involved in follicle growth, peak in their expression during early prehierarchical development. This agrees with previous results[20, 49]. Interestingly, both FSHR and TGFBR1 show a prominent peak at 8–10 mm, indicating that follicles immediately post-recruitment may have a heightened sensitivity to the ligands of these receptors at this stage. As both receptors activate pathways leading to cell growth, proliferation and differentiation, higher expression at those stages is not surprising. SMAD3, a known downstream signal mediator of TGFB family receptors demonstrates a very similar pattern of expression to TGFBR1. However, TGFBR1 and SMAD 3 did not show a significant effect in response to ad libitum feeding, whereas FSHR was significantly downregulated in the F1 follicle under ad libitum feeding. It is most likely that, as the lower FSHR expression shown in ad libitum fed broilers is more comparable with previous studies[53], the increased expression in feed restricted birds leads to negative feedback resulting from steroidogenic factors. Little functional significance has been placed on FSH control in the F1 and further investigation is warranted to explore the potential roles for FSHR in this follicle in light of the results reported here. MOSPD1 has been implicated in mesenchymal cell differentiation[50] and is upregulated in ovarian cancer[54]. BioLayout Express analysis showed it clustered with FSHR in broiler breeders and QPCR profiling in layers corroborated this. MOSPD1 is a membrane-associated protein[55] and may be involved in supporting or mediating signal transduction from the FSH receptor but further work will be required to determine this.

GDF9 was not identified through the original microarray analysis but was included due to its location near the putative QTL for follicle number in the chicken and because studies in sheep report an association between mutations in GDF9 and increased ovarian follicle number and ovulation rate[56, 57]. Despite showing little change in expression between tissues in the initial screen in layers, GDF9 does show significant downregulation in response to ad libitum feeding in broiler breeders in the stroma and in 6–8 mm follicles (P = 0.005). In conjunction with results from mamalian studies this result would imply an inhibitory effect on follicle number. It is interesting to note that the expression profile for GDF9 in broiler breeders, regardless of diet, is comparable with other species[5861], indicating a high level of inter-species conservation for this gene. Reported inter-species sequence conservation from the UCSC Chicken Genome Browser supports this, with sheep being most comparable in terms of exon coverage. Further investigation is underway to determine if there are mutations in the chicken, as there are in sheep, that might be associated with multiple ovulation.

SLIT3 and VDAC1 have both been shown to be involved in pro-apoptotic signalling[4346] and are located in the putative QTL for follicle number on chromosome 13. SLIT3 is also involved in ovary and follicle development in sheep[62] and its expression profile in layers is consistent with phases of increased apoptosis. Expression profiling of SLIT3 and VDAC1 in BioLayout Express for broiler breeders is consistent with the layer QPCR profiling. However, there was no significant differential expression either between tissues or dietary regimes in the broiler breeder QPCR validation for either candidate.

YAP1 is believed to be involved in cell survival signalling through regulation of the p53 signalling pathway[51]. The BioLayout Express profile of the broiler breeders suggests that it is upregulated in late prehierarchical follicles. However, profiling in layers shows higher expression in early prehierarchical follicles and the F5 follicle, where pro-survival signals would be expected to occur more prominently. Upregulation in ad libitum fed birds could not be validated by QPCR. This does not negate a role for YAP1 in follicular development, however it is unlikely to be responsible for multiple ovulation in broiler breeders.

GULP1 is expressed in macrophages and is involved in engulfment of apoptotic cells[47, 48]. Profiling in layers is consistent with this activity. While this is not likely to be a candidate for follicle selection, it does highlight the transitional stages of the follicle as it progresses through development.

RIGG1740, KRT75 and SPTY2D1 were also investigated for a potential role in follicle development, however their expression profiles in layers, in conjunction with the level of available evidence (summarised in Table5) did not indicate a central role in follicle recruitment.

Conclusions and further work

Our prime candidates, PDGFRL, GDF9 and FSHR, although only indirectly linked, all have strong cases for further investigation. GDF9 and FSHR are not novel candidates, indeed, FSHR was intially included in this study as a form of positive control and the identification of a previously unreported dietary effect on its expression was unexpected. PDGFRL however, is a novel candidate, and its implicated role in regulation of steriodogenesis, along with its response to ad libitum feeding makes it of primary importance.

Further investigation of PDGFRL, as well as FSHR and GDF9, is clearly warranted, and would yield many valuable insights. For example, work to determine localisation of expression of PDGFRL within the different cell types in the follicle wall is clearly needed to further substantiate its proposed role in steroid-based feedback. Successful examination of these candidates should bring us one step closer to solving the problem of multiple ovulation and allow for the prospect of relaxing feed restriction to improve the welfare of broiler breeder chickens.

Declarations

Acknowledgements

The FR vs. AL microarray study was funded by Defra. The BBSRC, Biosciences KTN and Aviagen contributed funding for a CASE Studentship to take the work forward beyond the original study. The Roslin Institute is supported by a core strategic grant from the BBSRC.

Authors’ Affiliations

(1)
The Roslin Institute & R(D)SVS, University of Edinburgh, Easter Bush

References

  1. Mackay J: Biology of breeding poultry; Chapter 1: The Genetics of Modern Poultry. 2009, Cambridge: CABI North American Office
  2. Decuypere E, Hocking PM, Tona K, Onagbesan O, Bruggeman V, Jones EKM, Cassy S, Rideau N, Metayer S, Jego Y, et al: Broiler breeder paradox: a project report. World Poultry Sci J. 2006, 62 (3): 443-453. 10.1079/WPS2005107.View Article
  3. Hocking PM, Bernard R, Robertson GW: Effects of low dietary protein and different allocations of food during rearing and restricted feeding after peak rate of lay on egg production, fertility and hatchability in female broiler breeders. Br Poult Sci. 2002, 43 (1): 94-103. 10.1080/00071660120109908.View ArticlePubMed
  4. Hocking PM, Robertson GW: Ovarian follicular dynamics in selected and control (relaxed selection) male- and female-lines of broiler breeders fed ad libitum or on restricted allocations of food. Br Poult Sci. 2000, 41 (2): 229-234. 10.1080/713654904.View ArticlePubMed
  5. Hocking PM: Biology of breeding poultry; Chapter 17: Feed Restriction. 2009, Cambridge: CABI North American OfficeView Article
  6. Hocking PM: High-fibre pelleted rations decrease water intake but do not improve physiological indexes of welfare in food-restricted female broiler breeders. Br Poult Sci. 2006, 47 (1): 19-23. 10.1080/00071660500468041.View ArticlePubMed
  7. Hocking PM, Maxwell MH, Robertson GW, Mitchell MA: Welfare assessment of broiler breeders that are food restricted after peak rate of lay. Br Poult Sci. 2002, 43 (1): 5-15. 10.1080/00071660120109818.View ArticlePubMed
  8. Ciccone NA, Dunn IC, Sharp PJ: Increased food intake stimulates GnRH-L glycoprotein hormone alpha-subunit and follistatin mRNAs, and ovarian follicular numbers in laying broiler breeder hens. Domest Anim Endocrinol. 2007, 33 (1): 62-76. 10.1016/j.domaniend.2006.04.008.View ArticlePubMed
  9. Bruggeman V, Vanmontfort D, Renaville R, Portetelle D, Decuypere E: The effect of food intake from two weeks of age to sexual maturity on plasma growth hormone, insulin-like growth factor-I, insulin-like growth factor-binding proteins, and thyroid hormones in female broiler breeder chickens. Gen Comp Endocrinol. 1997, 107 (2): 212-220. 10.1006/gcen.1997.6917.View ArticlePubMed
  10. Monaghan P, Nager RG: Why don’t birds lay more eggs?. Trends Ecol Evol. 1997, 12 (7): 270-274. 10.1016/S0169-5347(97)01094-X.View ArticlePubMed
  11. Sharp PJ: Control of Reproduction in Chickens. Encyclopaedia of Reproduction. 1999, London: Academic Press, 572-580. Volume 1
  12. Richards MP, Rosebrough RW, Coon CN, McMurtry JP: Feed intake regulation for the female broiler breeder: in theory and in practice. J Appl Poultry Res. 2010, 19 (2): 182-193. 10.3382/japr.2010-00167.View Article
  13. Bahr JM, Johnson AL: Regulation of the follicular hierarchy and ovulation. J Exp Zool. 1984, 232 (3): 495-500. 10.1002/jez.1402320316.View ArticlePubMed
  14. Fortune JE: Ovarian follicular-growth and development in mammals. Biol Reprod. 1994, 50 (2): 225-232. 10.1095/biolreprod50.2.225.View ArticlePubMed
  15. Hirshfield AN: International review of cytology: A Survey of Cell Biology Chapter 2: Development of Follicles in the Mammalian Ovary. 1991, London: Academic Press
  16. Wyburn GM, Aitken RNC, Johnston HS: Ultrastructure of zona radiata of ovarian follicle of domestic fowl. J Anat. 1965, 99: 469-PubMed CentralPubMed
  17. Knight PG, Al-Musawi SL, Lovell TM, Gladwell RT: Biology of breeding poultry; Chapter 7: Control of Follicular Development: Intra-Ovarian Actions of Transforming Growth Factor-Beta (TGF-B) Superfamily Members. 2009, Cambridge: CABI North American Office
  18. Gilbert AB, Perry MM, Waddington D, Hardie MA: Role of atresia in establishing the follicular hierarchy in the ovary of the domestic hen (Gallus-Domesticus). J Reprod Fertil. 1983, 69 (1): 221-227. 10.1530/jrf.0.0690221.View ArticlePubMed
  19. Etches RJ, Schoch JP: A mathematical representation of the ovulatory cycle of the domestic hen. Br Poult Sci. 1984, 25 (1): 65-76. 10.1080/13632758408454843.View ArticlePubMed
  20. You S, Bridgham JT, Foster DN, Johnson AL: Characterization of the chicken follicle-stimulating hormone receptor (cFSH-R) complementary deoxyribonucleic acid, and expression of cFSH-R messenger ribonucleic acid in the ovary. Biol Reprod. 1996, 55 (5): 1055-1062. 10.1095/biolreprod55.5.1055.View ArticlePubMed
  21. Lussier JG, Matton P, Dufour JJ: Growth-rates of follicles in the ovary of the cow. J Reprod Fertil. 1987, 81 (2): 301-307. 10.1530/jrf.0.0810301.View ArticlePubMed
  22. Scaramuzzi RJ, Turnbull KE, Nancarrow CD: Growth of graafian-follicles in cows following luteolysis induced by the prostaglandin-F2-alpha analog, cloprostenol. Aust J Biol Sci. 1980, 33 (1): 63-69.PubMed
  23. Hodgen GD, Kenigsburg D, Collins RL, Schenken RS: Selection of the dominant ovarian follicle and hormonal enhancement of the natural cycle. Ann N Y Acad Sci. 1985, 442: 23-37. 10.1111/j.1749-6632.1985.tb37502.x.View ArticlePubMed
  24. Dunn IC, Ciccone NA, Joseph NT: Biology of breeding poultry; Chapter 6: Endocrinology and Genetics of the Hypothalamic-Pituitary-Gonadal Axis. 2009, Cambridge: CABI North American Office
  25. Dunn IC, Sharp PJ: Photo-induction of hypothalamic gonadotrophin releasing hormone-I mRNA in the domestic chicken: a role for oestrogen?. J Neuroendocrinol. 1999, 11 (5): 371-375.View ArticlePubMed
  26. Johnson AL, Vantienhoven A: Plasma-Concentrations of 6 steroids and Lh during the ovulatory cycle of the Hen, gallus-domesticus. Biol Reprod. 1980, 23 (2): 386-393. 10.1095/biolreprod23.2.386.View ArticlePubMed
  27. Bahr JM, Wang SC, Huang MY, Calvo FO: Steroid concentrations in isolated theca and granulosa layers of preovulatory follicles during the ovulatory cycle of the domestic Hen. Biol Reprod. 1983, 29 (2): 326-334. 10.1095/biolreprod29.2.326.View ArticlePubMed
  28. Etches RJ, Duke CE: Progesterone, androstenedione and estradiol content of theca and granulosa tissues of the 4 largest ovarian follicles during the ovulatory cycle of the Hen (Gallus-Domesticus). J Endocrinol. 1984, 103 (1): 71-76. 10.1677/joe.0.1030071.View ArticlePubMed
  29. Cunningham FJ: Ovulation in the hen: neuroendocrine control. Oxf Rev Reprod Biol. 1987, 9: 96-136.PubMed
  30. Johnson AL, Bridgham JT, Wagner B: Characterization of a chicken luteinizing hormone receptor (cLH-R) complementary deoxyribonucleic acid, and expression of cLH-R messenger ribonucleic acid in the ovary. Biol Reprod. 1996, 55 (2): 304-309. 10.1095/biolreprod55.2.304.View ArticlePubMed
  31. Tilly JL, Kowalski KI, Johnson AL: Cytochrome-P450 side-chain cleavage (P450scc) in the Hen ovary.2. P450scc Messenger-Rna, Immunoreactive Protein, and Enzyme-Activity in developing Granulosa-Cells. Biol Reprod. 1991, 45 (6): 967-974. 10.1095/biolreprod45.6.967.View ArticlePubMed
  32. Tilly JL, Kowalski KI, Johnson AL: Stage of ovarian follicular development associated with the initiation of steroidogenic competence in avian granulosa-cells. Biol Reprod. 1991, 44 (2): 305-314. 10.1095/biolreprod44.2.305.View ArticlePubMed
  33. Lovell TM, Gladwell RT, Groome NP, Knight PG: Modulatory effects of gonadotrophins and insulin-like growth factor on the secretion of inhibin A and progesterone by granulosa cells from chicken preovulatory (F1-F3) follicles. Reproduction. 2002, 123 (2): 291-300.View ArticlePubMed
  34. Team RDC: R: A Language and Environment for Statistical Computing. 2011, Vienna: R Foundation for Statistical Computing
  35. Theocharidis A, van Dongen S, Enright AJ, Freeman TC: Network visualization and analysis of gene expression data using BioLayout Express(3D). Nat Protoc. 2009, 4 (10): 1535-1550. 10.1038/nprot.2009.177.View ArticlePubMed
  36. Aviagen J: Parent Stock Management Manual: ROSS 308. 2008, Newbridge: Aviagen Ltd
  37. Zakaria AH, Sakai H, Imai K: Time of follicular transformation to the rapid growth-phase in relation to the ovulatory cycle of laying Hens. Poult Sci. 1984, 63 (5): 1061-1063. 10.3382/ps.0631061.View ArticlePubMed
  38. Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 2000, 132: 365-386.PubMed
  39. Smyth GK, Speed T: Normalization of cDNA microarray data. Methods. 2003, 31 (4): 265-273. 10.1016/S1046-2023(03)00155-5.View ArticlePubMed
  40. Dunn IC, Wilson PW, Lu Z, Bain MM, Crossan CL, Talbot RT, Waddington D: New hypotheses on the function of the avian shell gland derived from microarray analysis comparing tissue from juvenile and sexually mature hens. Gen Comp Endocrinol. 2009, 163 (1–2): 225-232.View ArticlePubMed
  41. Benjamini Y, Hochberg Y: Controlling the false discovery rate-a practical and powerful approach to multiple testing. J Roy Stat Soc B Met. 1995, 57 (1): 289-300.
  42. Payne RW, Murray DA, Harding SA, Baird DB, Soutar DM: An Introduction to GenStat for Windows (14th Edition). 2011, Hemel Hempstead: VSN International
  43. Dickinson RE, Duncan WC: The SLIT-ROBO pathway: a regulator of cell function with implications for the reproductive system. Reproduction. 2010, 139 (4): 697-704. 10.1530/REP-10-0017.PubMed CentralView ArticlePubMed
  44. Marlow R, Strickland P, Lee JS, Wu XY, PeBenito M, Binnewies M, Le EK, Moran A, Macias H, Cardiff RD, et al: SLITs suppress tumor growth in vivo by silencing Sdf1/Cxcr4 within breast epithelium. Cancer Res. 2008, 68 (19): 7819-7827. 10.1158/0008-5472.CAN-08-1357.PubMed CentralView ArticlePubMed
  45. Keinan N, Tyomkin D, Shoshan-Barmatz V: Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis. Mol Cell Biol. 2010, 30 (24): 5698-5709. 10.1128/MCB.00165-10.PubMed CentralView ArticlePubMed
  46. Lan CH, Sheng JQ, Fang DC, Meng QZ, Fan LL, Huang ZR: Involvement of VDAC1 and Bcl-2 family of proteins in VacA-induced cytochrome c release and apoptosis of gastric epithelial carcinoma cells. J Dig Dis. 2010, 11 (1): 43-49. 10.1111/j.1751-2980.2009.00412.x.View ArticlePubMed
  47. Park SY, Kim SY, Kang KB, Kim IS: Adaptor protein GULP is involved in stabilin-1-mediated phagocytosis. Biochem Biophys Res Commun. 2010, 398 (3): 467-472. 10.1016/j.bbrc.2010.06.101.View ArticlePubMed
  48. Osada Y, Sunatani T, Kim IS, Nakanishi Y, Shiratsuchi A: Signalling pathway involving GULP, MAPK and Rac1 for SR-BI-induced phagocytosis of apoptotic cells. J Biochem. 2009, 145 (3): 387-394. 10.1093/jb/mvn176.View ArticlePubMed
  49. Paradis F, Novak S, Murdoch GK, Dyck MK, Dixon WT, Foxcroft GR: Temporal regulation of BMP2, BMP6, BMP15, GDF9, BMPR1A, BMPR1B, BMPR2 and TGFBR1 mRNA expression in the oocyte, granulosa and theca cells of developing preovulatory follicles in the pig. Reproduction. 2009, 138 (1): 115-129. 10.1530/REP-08-0538.View ArticlePubMed
  50. Thaler R, Rumpler M, Spitzer S, Klaushofer K, Varga F: Mospd1, a new player in mesenchymal versus epidermal cell differentiation. J Cell Physiol. 2010, 226 (10): 2505-2515.View Article
  51. Espanel X, Sudol M: Yes-associated protein and p53-binding protein-2 interact through their WW and SH3 domains. J Biol Chem. 2001, 276 (17): 14514-14523.PubMed
  52. Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid-producing lineages. Genes Dev. 2008, 22 (23): 3255-3267. 10.1101/gad.1723908.PubMed CentralView ArticlePubMed
  53. Zhang CQ, Shimada K, Saito N, Kansaku N: Expression of messenger ribonucleic acids of luteinizing hormone and follicle-stimulating hormone receptors in granulosa and theca layers of chicken preovulatory follicles. Gen Comp Endocrinol. 1997, 105 (3): 402-409. 10.1006/gcen.1996.6843.View ArticlePubMed
  54. Puiffe ML, Le Page C, Filali-Mouhim A, Zietarska M, Ouellet V, Tonin PN, Chevrette M, Provencher DM, Mes-Masson AM: Characterization of ovarian cancer ascites on cell invasion, proliferation, spheroid formation, and gene expression in an in vitro model of epithelial ovarian cancer. Neoplasia. 2007, 9 (10): 820-829. 10.1593/neo.07472.PubMed CentralView ArticlePubMed
  55. Pall GS, Wallis J, Axton R, Brownstein DG, Gautier P, Buerger K, Mulford C, Mullins JJ, Forrester LM: A novel transmembrane MSP-containing protein that plays a role in right ventricle development. Genomics. 2004, 84 (6): 1051-1059. 10.1016/j.ygeno.2004.08.017.View ArticlePubMed
  56. Javanmard A, Azadzadeh N, Esmailizadeh AK: Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Vet Res Commun. 2011, 35 (3): 157-167. 10.1007/s11259-011-9467-9.View ArticlePubMed
  57. Silva BDM, Castro EA, Souza CJH, Paiva SR, Sartori R, Franco MM, Azevedo HC, Silva TASN, Vieira AMC, Neves JP, et al: A new polymorphism in the Growth and Differentiation Factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Anim Genet. 2011, 42 (1): 89-92. 10.1111/j.1365-2052.2010.02078.x.View ArticlePubMed
  58. Johnson PA, Dickens MJ, Kent TR, Giles JR: Expression and function of growth differentiation factor-9 in an oviparous species, Gallus domesticus. Biol Reprod. 2005, 72 (5): 1095-1100. 10.1095/biolreprod.104.036822.View ArticlePubMed
  59. Liu L, Ge W: Growth differentiation factor 9 and its spatiotemporal expression and regulation in the zebrafish ovary. Biol Reprod. 2007, 76 (2): 294-302. 10.1095/biolreprod.106.054668.View ArticlePubMed
  60. Garcia-Lopez A, Sanchez-Amaya MI, Halm S, Astola A, Prat F: Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): cellular localization, developmental profiles, and response to unilateral ovariectomy. Gen Comp Endocrinol. 2011, 174 (3): 326-334. 10.1016/j.ygcen.2011.09.011.View ArticlePubMed
  61. Elis S, Dupont J, Couty I, Persani L, Govoroun M, Blesbois E, Batellier F, Monget P: Expression and biological effects of bone morphogenetic protein-15 in the hen ovary. J Endocrinol. 2007, 194 (3): 485-497. 10.1677/JOE-07-0143.View ArticlePubMed
  62. Dickinson RE, Hryhorskyj L, Tremewan H, Hogg K, Thomson AA, McNeilly AS, Duncan WC: Involvement of the SLIT/ROBO pathway in follicle development in the fetal ovary. Reproduction. 2010, 139 (2): 395-407. 10.1530/REP-09-0182.PubMed CentralView ArticlePubMed

Copyright

© McDerment et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.