Figure 6From: Mining and characterization of ubiquitin E3 ligases expressed in the mouse testisLigase activity of putative E3s was validated by in vitro assays. Polyubiquitin chains were formed in the presence of UBE2D2 and Ube2k. Polyubiquitin chains were observed only in the presence of E1, E2, E3 and ubiquitin. GST protein was used as negative control (right lane6).Back to article page