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Table 1 Arrays analysed

From: Comparison of transcriptome technologies in the pathogenic fungus Aspergillus fumigatus reveals novel insights into the genome and MpkA dependent gene expression

No

Technique

Platform

Used strains

Growth conditions

Study

References

Accession

Technology

1

Microarray

TIGR (30 k v1)

Af293

5 × 106 conidia per ml, inoculated in complete media, for 17 hours, at 30–37°C, 160 rpm

Temperature shifts (30 to 37°C and 30 to 48°C)

[4]

ArrayExpress: E-MEXP-332

DNA amplicon microarray

2

Microarray

Custom (Roche)

Af293

108 conidia per ml, inoculated in potato dextrose media, for 24 hours, at 28°C, 160 rpm

Effects on growth in the presence of TSA

[18]

GEO: GSE19682

60mer oligonucleotide

3

Microarray

Febit

CEA17

106 conidia per ml, inoculated in minimal media, for 16 hours, at 37°C, 200 rpm

Comparison among wild type, ΔgprC, and ΔgprD mutant

[25]

Omnifung

in situ oligonucleotide

4

Microarray

TIGR (22 K v3)

ATTC 46645

107 conidia per ml, inoculated in Brian’s media, for 14 hours, at 37°C, 150 rpm

Comparison between planktonic and biofilm growth

[17]

GEO: GSE19430

in situ oligonucleotide

5

Microarray

Febit

CEA17

106 conidia per ml, inoculated in minimal media, for 16 hours, at 37°C, 200 rpm

Comparison between wild type and ΔmpkA mutant

[8]

Omnifung

in situ oligonucleotide

6

mRNA-Seq

Illumina

ATTC 46645 Af293 CEA10*

107 conidia per ml, inoculated in Brian’s media, for 14 hours, at 37°C, 150 rpm

Comparison between planktonic and biofilm growth

[12]

On enquiry

GAIIx

7

mRNA-Seq

Illumina

CEA17

106 conidia per ml, inoculated in minimal media, for 16 hours, at 37°C, 200 rpm

Comparison between wild type and ΔmpkA mutant

This study

ArrayExpress: E-MTAB-1236

GAIIx

8

Proteomic

2D-DIGE

ATTC 46645

106 conidia per ml, inoculated in minimal media, for 16 hours, at 37°C, 200 rpm

Design the A. fumigatus mycelial and mitochondrial proteome map

[21]

Omnifung

MALDI-TOF/TOF

9

Proteomic

2D-DIGE

CEA17

106 conidia per ml, inoculated in minimal media, for 16 hours, at 37°C, 200 rpm

Comparison between wild type and ΔmpkA mutant

This study

On enquiry

MALDI-TOF/TOF

  1. GEO: gene expression omnibus (http://www.ncbi.nlm.nih.gov/geo); Omnifung (http://www.omnifung.hki-jena.de); ArrayExpress (http://www.ebi.ac.uk/arrayexpress/). *CEA17 strain is the uracile auxotroph derived from the CEA10 strain.