Meiotic maps of sockeye salmon derived from massively parallel DNA sequencing
© Everett et al.; licensee BioMed Central Ltd. 2012
Received: 21 February 2012
Accepted: 23 September 2012
Published: 3 October 2012
Meiotic maps are a key tool for comparative genomics and association mapping studies. Next-generation sequencing and genotyping by sequencing are speeding the processes of SNP discovery and the development of new genetic tools, including meiotic maps for numerous species. Currently there are limited genetic resources for sockeye salmon, Oncorhynchus nerka. We develop the first dense meiotic map for sockeye salmon using a combination of novel SNPs found in restriction site associated DNA (RAD tags) and SNPs available from existing expressed sequence tag (EST) based assays.
We discovered and genotyped putative SNPs in 3,430 RAD tags. We removed paralogous sequence variants leaving 1,672 SNPs; these were combined with 53 EST-based SNP genotypes for linkage mapping. The map contained 29 male and female linkage groups, consistent with the haploid chromosome number expected for sockeye salmon. The female map contains 1,057 loci spanning 4,896 cM, and the male map contains 1,118 loci spanning 4,220 cM. Regions of conservation with rainbow trout and synteny between the RAD based rainbow trout map and the sockeye salmon map were established.
Using RAD sequencing and EST-based SNP assays we successfully generated the first high density linkage map for sockeye salmon.
KeywordsRAD sequencing Meiotic map Linkage map SNP
Meiotic maps are a critical component of the process of unraveling the complexities of the genomics of duplicated taxa. Whole genome duplication with concomitant re-diploidization is broadly acknowledged as the major driving force in adaptation and speciation[2–4]. Positive selection is enriched in duplicated genes[5, 6]. Sorting the relationships among orthologs, homologs, and homeologs in duplicated genomes can be both daunting and rewarding. Meiotic maps have been used to unravel the interactions among linkage, pseudolinkage, homeologous pairing, and interference in duplicated teleosts for more than three decades[7–9]. More recently, meiotic maps have become important for comparative genomics and association mapping studies.
Techniques that associate genotypes with phenotypes, whether disease, physiological, or ecological traits, can improve our understanding of local adaptation. Understanding how organisms interact with their local environments is increasingly important for conservation and management of natural populations. In order to understand these relationships, studies are turning to association-mapping techniques where thousands of ordered markers across a genome are scanned for association with a trait. The most powerful analyses require thousands of markers in a known order, as found in a genome sequence or high-density linkage map. Such tools are currently unavailable for most non-model organisms, though the rapid advance of high throughput sequencing techniques is facilitating their creation[12, 13].
The emergence of genotyping by sequencing (GBS),[14, 15] provides exciting opportunities to rapidly establish meiotic maps for non-model species. Previously, marker discovery posed a major hurdle to the development of high density linkage maps. Early studies generally included the mapping of only a few hundred markers. GBS now enables thousands of SNPs to be genotyped rapidly at relatively low cost[17, 18]. As a result, dense mapping studies are set to become common in wild populations, providing opportunities for association mapping, genome scans, and comparative mapping.
Salmonids with their residual polyploidy provide an excellent opportunity for study. Salmonids are an iconic family of fish, with both cultural and economic value, that naturally inhabit the North Pacific and North Atlantic Oceans. To date no published genome sequence for salmonids exists, though there are extensive SNP and EST resources for a few species e.g.,. Dense linkage maps, containing thousands of markers, currently only exist for rainbow trout Oncorhynchus mykiss[11, 16] and Atlantic salmon Salmo salar reviewed in[21, 22]; these species are intensively cultured for both food and sport fishing on several continents. The ongoing development of linkage maps in additional salmonid species has provided key insights into adaptation, sex determination, disease resistance, and other factors important to both conservation of wild populations and aquaculture. These maps also offer opportunities for comparative genomics[10, 15, 23–25]. A collateral benefit is that converting batteries of mapped SNPs to high-throughput genotyping assays will greatly enhance population genetic studies and genome scans that are becoming a backbone of conservation genomics.
A salmonid of special interest is the sockeye salmon O. nerka which is culturally important and supports some of the most valuable commercial fisheries by coastal nations throughout the subarctic North Pacific Ocean[27, 28]. Genomic resources are scant; no genetic maps exist for sockeye salmon. Although populations are robust in the northern climes, some cornerstone populations in Canada and the USA are threatened or endangered[29, 30]. As climate change intensifies, concerns intensify that thermal or disease challenges or both[33, 34] will further threaten commercial and subsistence economies. This threat, occurring during an era empowered by the advent of conservation genomics, precipitates a need for genomic resources to enable studies of genetic diversity, adaptive variation, and genotype-by-environmental interactions in sockeye salmon. Additionally, sockeye salmon exhibit a variety of unique life histories[28, 36], and additional genomic resources are necessary for determining the genetic basis for these diverse life history traits.
Our study had two goals. First was to discover thousands of novel SNPs in sockeye salmon using restriction site associated DNA sequencing (RAD) tags,[37–39]. Our second goal was to use these markers to generate a high density linkage map, with paired-end sequences used to expand template length to annotate as many SNPs as possible. We also incorporated EST-based SNPs, available through existing 5’-nuclease assays, into our genetic map because the substantial majority of RAD tags reside in non-coding sequences. We successfully discovered putative SNP-containing loci using RAD tag sequencing in a single family and used these to construct male and female consensus maps. The female map contains 1,057 markers and the male map contains 1,121 markers.
SNP discovery in parents
Experimental matings for SNP genotyping
Number of individuals
Segregating EST loci in each family
RAD sequencing, 5’-nuclease
RAD sequencing, 5’-nuclease
RAD tag and SNP discovery in the parents from family HX13-WL
Genotyping of offspring
Pooling strategy for Illumina sequencing
Illumina sequencing technology
Number of individuals per lane
Number of lanes
GAII paired end
GAII single end
HiSeq 2000 single end
In addition to RAD-based SNPs, we genotyped EST-based SNPs using 127 existing 5’-nuclease assays on parents and 45–141 offspring from each of the thirteen families (Table1). After filtering, each family contained between 44 and 57 segregating loci, with a total of 100 loci segregating across all families (Table2). Linkage relationships in these families were merged into the final map.
Both RAD and EST genotypes between parents and offspring were compared for inheritance error (genotypes in the offspring that do not correspond to the parental types), and erroneous genotypes were converted to missing data. Next the genotypes were screened for segregation distortion. The salmonid genome contains an ancient whole genome duplication and consequently contains many duplicate regions. The presence of these duplications has resulted in high false positive rates in previous SNP discovery efforts. In our data set, markers with significant segregation distortion (χ2 test, p < 0.05, deviation from 1:1 or 1:2:1 segregation patterns) may represent these paralogous sequence variants (PSVs), true distortion due to other genomic factors, or genotyping errors. Our genotyping method currently does not allow identification of genotyping error versus true distortion, thus we exclude all markers with significant distortion. Out of 3,530 putative SNPs, 1,758 (49%) showed significant segregation distortion and were removed from further analysis. The majority of these loci showed allelic distributions that indicated that they were likely PSVs (majority of all individuals heterozygous). All of the loci removed for segregation distortion were from the RAD tag sequences. Finally, after removing markers with greater than 25% missing data, 1,725 RAD and 5’-nuclease based SNPs were used in linkage analyses.
Meiotic map construction
Linkage mapping was carried out in two steps. First, the genotypes from the 53 EST loci segregating in family HX13-WL were combined with the RAD tag genotypes, and mapping was carried out on the combined RAD-EST dataset from family HX13-WL. Second, mapping was done on the EST loci that were segregating in the remaining families to maximize the number of ESTs that could be included in the final map (Table1). Linkage groups identified among these EST loci were merged with the HX13-WL linkage maps to create male and female consensus maps.
The female RAD-EST map from family HX13-WL contained 1,050 markers distributed among 29 linkage groups, consistent with the expected number of haploid chromosomes for sockeye salmon. The total map length was 4,943.7 cM. Twenty three markers failed to map and were excluded from further analysis. Additionally, this map contained two small fragments, each containing only two markers, which were discarded from further analysis.
The male RAD-EST map initially contained 28 linkage groups and a total length of 4,352.8 cM. Visual inspection of individual linkage groups and comparison to the female map revealed that one male linkage group corresponded to three female linkage groups (female linkage groups LG27, LG28, and LG29). Inspection of the recombination frequency matrix for the corresponding male and female linkage groups revealed that the male linkage group contained two linkage groups joined through an apparently spurious linkage relationship due to a single marker. No corresponding relationship could be observed in the female. This marker was removed, and the two linkage groups were separated. One of the divided male linkage groups matched to female LG28 and the other matched to the remaining two female groups (LG27, 29). Additionally, one female linkage group corresponded to two male linkage groups, LG7a and LG7b. LG7a contained only five markers and may represent a fragment of the larger LG7b. A number of markers within LG7a had LOD >4 with markers in 7b, suggesting that these are part of a single group. After corrections, the male RAD-EST linkage map contained 1,112 markers distributed among 29 linkage groups and eight small fragments containing only two or three markers. These fragments were discarded from further analysis. Sixteen markers failed to map. The male map length was 4,310.3 cM.
There were 29–35 segregating loci per parent in the EST data set from the additional families (Table1). As a result, only small linkage groups were established, consisting of between two and four markers per group with up to nine groups per family. Overlaps of two or more markers between groups among the families allowed some groups to be merged. After merging, 31 linkage relationships in the males and 28 in the females, consisting of between two and five markers each, were established. These fragments were compared to the RAD-based map from family HX13-WL and merged where two or more markers overlapped. In this fashion, thirteen additional EST loci were added to the consensus map, seven additional markers in the female map and six additional markers in the male. The remaining EST groups were excluded from further analysis.
There were 457 overlapping loci between the male and the female maps (40% of total female markers and 41% of total male markers) (Figure4, Additional files1,2,3,4,5,6 and7). In all cases the overlapping loci were assigned to the same linkage groups. The male map had two linkage groups (LG7a and LG7b) that corresponded to a single linkage group (LG7) in the female map, resulting in 28 unique male groups when compared to the female map. Examination of the recombination matrix for the corresponding female linkage group did not support splitting this group. Additionally, as noted above, male LG27 corresponds to female LG27 and LG29.
A number of linkage groups contain minor order discrepancies between the male and the female maps (Additional files1,2,3,4,5,6 and7). The markers contained these groups consistently in even when the LOD score is varied. Removal of each marker and repositioning using try.seq in both the male and the female was unable to resolve the order variation in these groups. In no cases did shared markers map to different linkage groups between the male and the female. Linkage groups containing marker order discrepancies include LG14, LG20, LG22, and LG27.
Recombination ratios among individual male and female linkage groups
Comparison to rainbow trout
Comparison between rainbow trout and sockeye salmon linkage groups []
Sockeye salmon linkage group
Rainbow trout linkage group
Number of hits
Paired-end sequencing of genomic DNA from six parents, to provide longer contigs for annotation, produced between 3,517,798 and 3,995,897 filtered reads per individual for assembly. Out of 6,860 potential contigs, 5,722 were successfully assembled with a minimum length of 150 bp. Average contig length was 211 ± 30 bp, with an average coverage value of 38.25 ± 33.76 reads.
Here we present a first-pass meiotic map for sockeye salmon that contains 29 linkage groups, the number expected based upon the sockeye salmon karyotype (2n = 58). The final map contains 1,718 SNP and EST loci distributed over more than 4,000 cM.
Linkage maps have been created for a number of salmonids using a variety of genotyping strategies. Until now, these maps generally required iterative attempts, through time, where markers were added and linkage groups converged as new genotyping techniques materialized. In many cases these early efforts were based on microsatellite and amplified fragment length polymorphisms (AFLPs) and had modest initial map sizes. Currently the most complete linkage maps are available for Atlantic salmon (more than 5,000 SNPs, microsatellites, and AFLPs) and rainbow trout (more than 4,000 SNPs, microsatellites, and AFLPs)[11, 16]. The initial map in coho salmon O. kisutch contained 281 microsatellite and AFLP markers. Mapping in brown trout Salmo trutta used 301 microsatellite and AFLP markers, and a map in arctic char Salvelinus alpinus contained 326 AFLP, microsatellite, and SNP markers. The pink salmon O. gorbuscha map of 460 loci emerged from iterations that also included fragments originating from PCR priming on short interspersed repeats SINEs, and randomly amplified polymorphic DNA RAPDs,[47, 48]. Microsatellites have advantages over the other marker types due to their high rates of heterozygosity and large number of alleles which facilitate mapping. However all of these strategies are based on the accurate sizing of specific fragments and yield genotypes that are extremely difficult to compare among laboratories and through time.
Recently, massively parallel sequencing has allowed the rapid development of hundreds to thousands of novel SNPs in many species. As in our study, these studies map hundreds to thousands of novel markers. The rapid advance of high throughput sequencing promises to greatly speed the generation of novel genetic maps for previously unmapped or minimally mapped species[1, 11, 49, 50]. The sequences from GBS are also easily converted to other methods of SNP genotyping, and genotypes obtained from SNPs from any method are generally comparable among laboratories and through time because they are based upon specific nucleotide differences rather than allele sizes.
Our map approaches the size of certain recent Atlantic salmon and rainbow trout genetic maps. The most recent Atlantic salmon map contains more than 5,000 SNPs. The previous map for Atlantic salmon was also large, containing approximately 1,500 AFLP, microsatellite, and SNP markers, similar to our map size[51–54]. The most recent rainbow trout maps contain 1,124 markers based on AFLPs, microsatellites and SNPs and 4,563 markers based upon RAD tags. The AFLP and microsatellite-based maps represent years of discovery and mapping.
Salmonids have different rates of recombination between the sexes[7, 43, 44, 54]. Rexroad et al. reported an overall ratio of female to male recombination in rainbow trout of 1.68:1, with recombination rates on individual chromosomes ranging from 0.73:1 to 12.22:1. In Atlantic salmon the overall recombination ratio was 1.38:1, and the individual female to male ratios varied from 0.88:1 to 7.39:1. Direct comparison of our male and female maps is inappropriate because our maps contained both shared markers and markers unique to each gender. Given that marker order is generally conserved in the shared markers, we compare the distances between the most proximal and distal of the shared markers between the male and female linkage groups to compare rates of recombination. Overall recombination rates in males were lower than in females, which can be observed in Figure5. The recombination ratio among shared markers on individual linkage groups ranged from 0.71:1 to 1.71:1 (Table4). Our findings are consistent with those in both rainbow trout and Atlantic salmon where a number of the male linkage groups were larger than the female groups[16, 22]. Current research in salmonids suggests that recombination in males is restricted to the telomeres[16, 22], thus our values are likely underestimates of the true recombination differences between males and females (See also below).
There are regional differences in recombination between the females and males (Figure4). In the female, markers recombine along the length of the linkage groups, while there are blocks of markers with little or no recombination in most of the male linkage groups. Large regions of non-recombinant markers have been observed in other salmonid species including Atlantic salmon and rainbow trout[11, 16, 22, 53]. Two of these studies suggest that these blocks of non-recombinant markers may be located near the centromeres[11, 53]; however, additional information is needed to confirm this assertion in sockeye salmon. While blocks of non-recombinant markers were present on most (23 out of 29 have regions of limited recombination) linkage groups in the male map, the blocks that we observed were smaller than those observed in Atlantic salmon or rainbow trout. Additionally, while overall recombination is reduced in the male as compared to the female, many of the markers shared between the genders are well spaced in both the male and the female (not contained within large, non-recombinant blocks). This may be an artifact of the OneMap analysis. OneMap uses maximum likelihood to assign phase to markers which are identical heterozygotes in the progeny and parents. This allows the inclusion of these markers in the linkage map but it is unclear how this analysis may interact with the limited recombination in the male However, despite the spacing of these markers, overall the male map had more limited recombination than the female (see Figure5). Inclusion of more families in future efforts would help to resolve marker spacing and order issues.
Our total map lengths are larger than previously observed in salmonids. The salmon genome size is estimated to be 2.4 to 3.0 × 109 bp which would correspond to a map length of approximately 3,000 cM (1 cM is generally estimated to be approximately one million bp). Several linkage groups such as LG6-F (Figure4) contain large distances between either individual markers or small clusters of markers and the remaining linkage group. These large breaks could be the result of weak linkage relationships. Additionally, four linkage groups contained discrepancies in the order determined between male and female (Additional files6 and7). Varying the LOD score had no effect on the assignment of markers to their specified group. Other efforts to resolve the order including dropping and using the try.seq function on each marker to optimize position were unsuccessful. While the order could be manually changed, the size of the linkage group was often greatly increased and large breaks between markers were introduced. Increasing the number of individuals mapped may reduce the distance between these and other markers by strengthening linkage relationships and eliminating error, resulting in more consistent measurement of genetic distance, as well as helping to resolve marker order in the four linkage groups. Inflated map lengths and marker order problems may also be a result of missing data or genotyping errors. Slate et al. demonstrated that, when genotyping error is present, markers tend to be assigned to correct chromosomes but with inflated map distances and order errors. We minimized genotyping error in several ways: our RAD data coverage is higher than the recommended coverage values for accurate GBS[15, 59], and markers with greater than 25% missing data, inheritance error, and significant segregation distortion were removed. However some errors may still remain. The effect of even a few errors is likely also compounded by our relatively small sample size (96). Increasing sample size to increase the number of recombination events would reduce the effect of genotyping error and give higher confidence in map lengths and order.
Our RAD tag loci, including both putative SNPs and monomorphic loci, were compared with those discovered for rainbow trout in Miller et al.. Using strict comparison criteria, we discovered more than 16,000 matches between the two data sets, including 46 shared markers between the maps (Table5, Additional files1234 and5). These matches represent only 42 percent of the total number of the rainbow trout RAD loci. A previous study reported 95.7 percent identity between rainbow trout and sockeye salmon; however, this study focused on similarity among coding regions. We compared anonymous regions of genomic DNA where rates of nucleotide divergence may be higher than those in coding regions. Due to the short length of the RAD tags, strict criteria for matches were imposed (full length, no more than 2 mismatches). This likely underestimates the number of true hits between the species. Salmonid species including rainbow trout, Atlantic salmon, arctic char and coho salmon have been shown to have large syntenic blocks among chromosome arms[43, 44, 51, 60]. Our results are consistent with this finding. A recent study by Faber-Hammond et al. demonstrated that the sockeye salmon neo-y chromosome corresponds to two rainbow trout chromosome arms from rainbow trout chromosome 8 and chromosome 2. Based on our comparison to the rainbow trout RAD map, we have corresponding matches on linkage groups LG9 and LG14 suggesting that one of these may contain the sockeye salmon neo-y chromosome. Further testing is needed to confirm this.
Longer contigs were assembled for more than 3,000 RAD tag alleles, and from these 669 (~22%) were annotated using a BLASTX search. We initially anticipated a much lower rate of annotation consistent with the percentage of protein coding sequence in salmonids (5% c.f. Cheng et al. 2005). Amores et al. points out that SbfI, the enzyme used to create our RAD libraries, targets GC rich regions and thus cuts frequently in coding regions. Out of these 669 annotations, 83 (12%) were transposable elements or related to transposition. Transposable elements are common in large genomes and it has been proposed that transposable elements may play a role in the speciation of salmonids[60, 63]. The remaining annotations span a variety of biological processes and functions (Figure6).
SNPs detected in RAD tags presented here should convert to high-throughput assays such as 5’-nuclease with high success. High-throughput genotyping of SNPs has emerged as an important tool for study of early life history, migration, and conservation of non-model organisms including sockeye salmon[64–66]. Many SNPs discovered in transcriptome sequence, an approach commonly used in the past, fail to amplify in high-throughput assays because of their proximity to intron boundaries. We attempted assays for 10 of our RAD-based SNPs; nine successfully amplified and performed in three test populations (Additional files1234 and5). Using SNPs derived from paired-end sequences enhances the opportunity to develop assays that require priming and probing templates that may approach 90 bp.
Currently, genomic resources for sockeye salmon are scant. Here we present a first pass meiotic map generated from 1,772 RAD and EST-based SNPs. This map and set of SNPs will prove to be an enormous resource for both association mapping and landscape and conservation genomics e.g.,[12, 68]. Of course, adding RAD tags and EST-based SNPs for more individuals and more families will further refine the map and improve its value for comparative mapping.
Thirteen pairs of sockeye salmon from Lake Aleknagik, Alaska, were collected during August, 2009, and used to create 13 full-sib families. Axillary fin clips were taken from all adults and preserved in ethanol for DNA extraction. All embryos were incubated, first in Alaska, and later at the hatchery facility at the University of Washington. After hatch, juveniles were reared in recirculating aquaria for one month at which time 100 individuals from each family were sampled as whole fry and stored in RNAlater (Ambion, Inc., Foster City, CA). Remaining individuals from each family were stored in ethanol. All animal care and use was carried out using methods approved by the International Animal Care and Use Committee (IACUC), under approved protocol 4229–01.
DNA extractions were carried out on all parents and offspring using DNeasy-96 kits (Qiagen, Valencia, CA) according to the manufacturers’ protocol. DNA was extracted from tail clips from 45–96 offspring from each family and fin-clips from all parents (Table1). Concentration of the extracted DNA was assessed using the Quant-iT PicoGreen dsDNA Assay (Life Technologies, Carlsbad, California) on a Victor D plate reader (PerkinElmer, Waltham, Massachusetts).
5’-nuclease SNP Genotyping and Parentage test
All individuals were genotyped for 127 previously determined EST-based SNPs[64, 69–71] following procedures for 5’ nuclease assays as described in Seeb et al.. Genotype calling was carried out using the SDS 2.3 software (Life Technologies, Carlsbad California) or the BioMark 3.0.2 software (Fluidigm, South San Francisco, California). Monomorphic assays and assays which failed to amplify in the families were excluded from further analysis. The remaining 5’-nuclease genotypes were successfully filtered for Mendelian inheritance error and segregation distortion as described below, and markers uninformative for mapping were removed. Occasional family-to-family contamination had been previously observed in our fish incubation and rearing system.
RAD library construction and sequencing
RAD library preparation was carried out on 96 offspring from one family and six parents (Table2) using the methods similar to those previously described[39, 40]. The parents were prepared for paired end sequencing to provide longer contigs for annotation and 5’nuclease assay design (see below); the offspring were prepared for single end sequencing. The methods are identical except for the size of the sheared library extracted from the gel, 400–800 bp for single end sequencing and 150–400 bp for paired end sequencing. The restriction enzyme SbfI was used to digest the DNA, and SbfI specific Illumina linkers each containing a unique barcode were ligated to each digested DNA sample as described in Miller et al.. Individual samples were pooled into libraries containing up to 16 individuals. Each library assessed for quality and concentration using a Bioanalyzer DNA 1000 kit (Agilent Technologies, Santa Clara, California), and final concentration was determined by the Bioanalyzer software (Agilent Technologies, Santa Clara, California).
Ten nanomoles of each library in EB (Qiagen, Valencia, California) and 0.1% Tween 20 were sequenced at the University of Oregon High Throughput Sequencing Facility. The parents were sequenced using paired-end technology at 2 × 80 bp on the Illumina Genome Analyzer II (GAII). All offspring were first single end sequenced at 80 bp on the GAII. Another round of sequencing was required to achieve satisfactory depth of coverage; the second round was conducted on the Illumina HiSeq2000 (Table3), which produces 100 bp reads.
All sequence analysis for parents and offspring was carried out using Perl scripts (Perl scripts used are available from Miller et al. ) and the publically available software programs Bowtie version 0.12.7 and Novoalign version 2.07 (http://www.novocraft.com). First the sequences were filtered for quality. The last ten base pairs from each sequence were removed from GAII sequences (paired end and single end); subsequently any reads with less than 80% chance of being error free were discarded from further analysis. Sequences obtained from the HiSeq2000 were trimmed to 70 bp by removing the last 30 bp (to match the GAII sequences), and any reads with less than 80% chance of being error free were discarded. The 80% threshold was determined using the Phred quality score of each read. The quality value at each nucleotide was converted to a probability score and summed across the read length and if the sum fell below 80% the read was discarded. All quality filtered data was de-multiplexed into separate sequence files for each individual using the barcodes contained in the Illumina linker ligated during library preparation (see above). These procedures were applied to all GAII single end, GAII paired end, and HiSeq2000 data. The data for each offspring from the GAII and the HiSeq2000 was combined for all further analysis. SNP discovery was then carried out using only parental sequence data.
Using Perl scripts, the set of unique RAD tag sequences for each parent was identified, and the count of occurrences for each unique sequence within each parent was obtained. After labeling each sequence with the parent of origin, the set of sequences from both parents were combined for alignment in Novoalign. The combined set of RAD tag sequences were self-aligned in Novoalign. The software was set to carry out an exhaustive search, returning up to 40 alignments per unique sequence. We set a maximum alignment score of 125. The results of this alignment were filtered to maximize true (non-paralogous) loci shared between parents. These loci included monomorphic and SNP containing loci, as well as some paralogs. The loci were filtered using methods adapted from Miller et al.. In order to filter the sequences, the script compares each alignment score to a threshold value, compares the number of reads at each alignment, and finally compares the number of alignments within and between each parent. The Perl script to identify loci takes into account alignment score, coverage, and the number of identified alignments both between and within parents. For this study a minimum alignment score of 30, with no more than one alternative allele in either parent was used. The results of this search were filtered to include only the sequences containing putative SNPs, and these were output in a FASTA file for use as a reference sequence for Bowtie alignment.
SNP genotyping in offspring
The sequences containing putative SNPs in the parents were used as a reference set in a Bowtie alignment, formatted as “allele 1” and “allele 2” for each putative SNP. Quality filtered sequences from all offspring and the two parents were aligned to this reference, with Bowtie set to only align reads which matched the reference perfectly. The output of this alignment was filtered using custom Perl scripts, counting the number of matches to each allele for each individual. These allele counts were exported for conversion into genotype calls in Excel.
SNP genotype calls from the allele counts were made using methods similar to those in Nielsen et al.. A threshold of 10 reads from both alleles (sum of both alleles) was required for genotyping; any locus with less than ten reads was called as missing. This threshold was determined experimentally by testing various threshold values on the data set and examining the resulting calls for inheritance error, segregation distortion, and the number of missing markers, and subsequently comparing these values between thresholds. During SNP discovery each allele was designated as allele 1 or allele 2. For all loci, allele 1 was designated as the reference allele, thus in some cases the alternative allele may have higher read counts than the reference allele. For each locus, a heterozygous genotype was called if the read count for the non-reference allele was between 28%-80% of the total count for both alleles. Otherwise a homozygous genotype was called. Certain combinations of allele counts may still result in a miscalled allele, however, such a miscall in the parents would result in a mismatch in alleles between parents and offspring. All data was screened for such inheritance error (see below) and markers with an excess of these errors were removed from the analysis.
Next, parent and offspring genotype calls were compared to check for inheritance error, where the genotypes of the progeny do not match the expected genotypes based on the parental genotypes. If found, these genotype calls were converted to missing data. The informative genotype calls from existing sockeye 5’-nuclease assays were added to the data set. The data was checked for segregation distortion from 1:1 or 1:2:1 ratio using a χ2test. Markers exhibiting significant levels of segregation distortion were removed from the analysis. Finally, markers for which one parent or more than 25% of offspring genotype calls were missing were removed, and the genotypes were converted into OneMap format[56, 74].
Linkage analysis was carried out using the R package OneMap version2.0-1. OneMap uses a maximum likelihood analysis to assign phase to identical heterozygous progeny genotypes, allowing the inclusion of these markers in the linkage map. Male and female linkage maps were generated separately. All linkage map ordering was carried out using the Kosambi map function[8, 75, 76]. Data was imported in outcross format. Next, the recombination fraction between all pairs of markers was calculated using the rf.2pts algorithm. Linkage groups were formed using the group command in two stages. First, linkage groups were formed with LOD = 12 and a maximum recombination fraction of 0.01. These groups were ordered using either the compare function or the order.seq function depending on the number of markers. Groups with up to seven markers were ordered with the compare function, groups containing more than seven were ordered with order.seq. This identified markers which overlap in position. One marker from each position with the fewest missing genotypes was selected to use in the next step of map construction. Next, linkage groups were formed with LOD = 6 and the default recombination fraction. Order.seq was used to place markers in their initial order. After initial ordering, ripple.seq was used to test the marker order, with a sliding window of four markers, and the order was changed when necessary. The marker orders were also inspected visually using rf.graph.table, which plots a heat map of the LOD score and recombination frequency, for ordering errors too far apart to detect with ripple.seq and made manual changes when necessary. Finally, the markers removed in the LOD = 12 analysis were individually added back to each linkage group using the try.seq and ripple.seq functions to finalize marker order. The male and female linkage maps were compared for marker order among the shared markers. Where discrepancies were found the individual marker was removed using drop.seq, and then added back to the map using try.seq to examine optimal marker position. In cases where no consensus order could be established this is noted in the text.
Linkage relationships were obtained among the segregating 5’-nuclease loci in each of the 12 families and in both the RAD tag data and the 5’-nuclease assays in the sequences family. The individual male and female maps from each family were combined where possible using MERGE, part of the LINKMFeX package.
Comparison to rainbow trout RAD tags
All putative loci discovered in the HX13-WL parents were compared to the rainbow trout RAD tags in Miller et al. using BLASTN (BLAST version 2.2.25). For unmapped loci, only full length (59 bp) hits with no more than one mismatch were considered. For loci used for either map, the stringency was stepped down and the map locations were compared. If hits occurred in one to two linkage groups, consistent with syntenic matches, they were kept. In this way, hits longer than 56 bp and containing no gaps and no more than four mismatches were considered matches. Microsoft Excel was used to identify shared tags that were present in both rainbow trout and sockeye salmon linkage maps. Tag positions were compared between the rainbow trout and sockeye linkage maps and overlapping map hits were counted.
Paired End assembly
Paired-end sequence data from the parents was assembled to generate longer sequences for use in annotation of RAD tags and as a resource for 5’ nuclease assay design. Paired-end assembly was carried out using custom Perl scripts and the publically available program Velvet version 1.1.06. The Perl script matches each sequence in the FASTA file generated during SNP discovery, containing the 6,860 SNP alleles, against the forward end (paired-end 1) paired-end file and pulls both matching sequences and their pairs and writes them into a temporary data file. Both alleles of each SNP discovered were used as the template for the paired-end sequence assembly. The complete set of paired, filtered reads from all six individuals was compared to the SNP-containing FASTA file. The temporary file is then run through Velvet, and the resulting output is added to the results file. This procedure is repeated iteratively until all the sequences have been compared. Velvet is set with a k-mer value of 21 and a minimum contig length of 150 bp. All other Velvet settings are set to default values. For any contig not meeting the 150 bp minimum, a placeholder value is inserted to indicate the missing sequence. After assembly, duplicate contigs were removed.
Contigs from the paired-end assembly were subjected to a BLAST search against the Swiss-Prot database using BLASTX. An e-value of less than 10-4 was required to accept the annotation. Microsoft Access and the complete set of GO terms for the Swiss-Prot database (NCBI version 2011_11) were used to associate sequence annotation with their GOSlim term. Terms were assigned for both biological process and cellular function.
The authors would like to thank the researchers at Lake Aleknagik for aid in rearing the crosses and Carita Pascal for invaluable support and advice in the lab. This project was completed with equal contributions from the Gordon and Betty Moore Foundation and the Bristol Bay Regional Salmon Development Association. MRM was funded by an NSF Graduate Research Fellowship and thanks Chris Doe for invaluable support.
- Amores A, Catchen J, Ferrara A, Fontenot Q, Postlethwait JH: Genome evolution and meiotic maps by massively parallel DNA sequencing: Spotted gar, an outgroup for the teleost genome duplication. Genetics. 2011, 188 (4): 799-U779. 10.1534/genetics.111.127324.PubMed CentralView ArticlePubMedGoogle Scholar
- Lynch M, Conery JS: The origins of genome complexity. Science. 2003, 302 (5649): 1401-1404. 10.1126/science.1089370.View ArticlePubMedGoogle Scholar
- Postlethwait JH: The zebrafish genome in context: Ohnologs gone missing. J Exp Zool B Mol Dev Evol. 2007, 308B (5): 563-577. 10.1002/jez.b.21137.View ArticleGoogle Scholar
- Ohno S: Gene duplication and the uniqueness of vertebrate genomes circa 1970–1999. Semin Cell Dev Biol. 1999, 10 (5): 517-522. 10.1006/scdb.1999.0332.View ArticlePubMedGoogle Scholar
- Kostka D, Hahn MW, Pollard KS: Noncoding sequences near duplicated genes evolve rapidly. Genome Biol Evol. 2010, 2: 518-533. 10.1093/gbe/evq037.PubMed CentralView ArticlePubMedGoogle Scholar
- Soltis DE, Albert VA, Leebens-Mack J, Bell CD, Paterson AH, Zheng CF, Sankoff D, DePamphilis CW, Wall PK, Soltis PS: Polyploidy and angiosperm diversification. Am J Bot. 2009, 96 (1): 336-348. 10.3732/ajb.0800079.View ArticlePubMedGoogle Scholar
- Allendorf FW, Danzmann RG: Secondary tetrasomic segregation of MDH-B and preferential pairing of homeologues in rainbow trout. Genetics. 1997, 145 (4): 1083-1092.PubMed CentralPubMedGoogle Scholar
- Thorgaard GH, Allendorf FW, Knudsen KL: Gene-centromere mapping in rainbow-trout - high interference over long map distances. Genetics. 1983, 103 (4): 771-783.PubMed CentralPubMedGoogle Scholar
- Wright JE, Johnson K, Hollister A, May B: Meiotic models to explain classical linkage, pseudolinkage, and chromosome pairing in tetraploid derivative salmonid genomes. Isozymes. 1983, 10: 239-260.PubMedGoogle Scholar
- Li J, Phillips RB, Harwood AS, Koop BF, Davidson WS: Identification of the sex chromosomes of brown trout (Salmo trutta) and their comparison with the corresponding chromosomes in Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Cytogenet Genome Res. 2011, 133 (1): 25-33. 10.1159/000323410.View ArticlePubMedGoogle Scholar
- Miller MR, Brunelli JP, Wheeler PA, Liu S, Rexroad CE, Palti Y, Doe CQ, Thorgaard GH: A conserved haplotype controls parallel adaptation in geographically distant salmonid populations. Mol Ecol. 2011, 21: 237-249. 10.1111/j.1365-1294X.2011.05305.x.View ArticlePubMedGoogle Scholar
- Allendorf FW, Hohenlohe PA, Luikart G: Genomics and the future of conservation genetics. Nat Rev Genet. 2010, 11 (10): 697-709.View ArticlePubMedGoogle Scholar
- Slate J, Gratten J, Beraldi D, Stapley J, Hale M, Pemberton JM: Gene mapping in the wild with SNPs: guidelines and future directions. Genetica. 2009, 136 (1): 97-107. 10.1007/s10709-008-9317-z.View ArticlePubMedGoogle Scholar
- Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE: A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS One. 2011, 6 (5): e19379-10.1371/journal.pone.0019379.PubMed CentralView ArticlePubMedGoogle Scholar
- Davey JW, Hohenlohe PA, Etter PD, Boone JQ, Catchen JM, Blaxter ML: Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet. 2011, 12 (7): 499-510. 10.1038/nrg3012.View ArticlePubMedGoogle Scholar
- Rexroad CE, Palti Y, Gahr SA, Vallejo RL: A second generation genetic map for rainbow trout (Oncorhynchus mykiss). BMC Genet. 2008, 9: 74-PubMed CentralView ArticlePubMedGoogle Scholar
- Pfender WF, Saha MC, Johnson EA, Slabaugh MB: Mapping with RAD (restriction-site associated DNA) markers to rapidly identify QTL for stem rust resistance in Lolium perenne. Theor Appl Genet. 2011, 122 (8): 1467-1480. 10.1007/s00122-011-1546-3.View ArticlePubMedGoogle Scholar
- Emerson KJ, Merz CR, Catchen JM, Hohenlohe PA, Cresko WA, Bradshaw WE, Holzapfel CM: Resolving postglacial phylogeography using high-throughput sequencing. Proc Natl Acad Sci USA. 2010, 107 (37): 16196-16200. 10.1073/pnas.1006538107.PubMed CentralView ArticlePubMedGoogle Scholar
- Slate J, Santure AW, Feulner PGD, Brown EA, Ball AD, Johnston SE, Gratten J: Genome mapping in intensively studied wild vertebrate populations. Trends Genet. 2010, 26 (6): 275-284. 10.1016/j.tig.2010.03.005.View ArticlePubMedGoogle Scholar
- Koop BF, von Schalburg KR, Leong J, Walker N, Lieph R, Cooper GA, Robb A, Beetz-Sargent M, Holt RA, Moore R, et al: A salmonid EST genomic study: genes, duplications, phylogeny and microarrays. BMC Genomics. 2008, 9: 545-10.1186/1471-2164-9-545.PubMed CentralView ArticlePubMedGoogle Scholar
- Davidson WS, Koop BF, Jones SJM, Iturra P, Vidal R, Maass A, Jonassen I, Lien S, Omholt SW: Sequencing the genome of the Atlantic salmon (Salmo salar). Genome Biol. 2010, 11 (9): 403-PubMed CentralPubMedGoogle Scholar
- Lien S, Gidskehaug L, Moen T, Hayes BJ, Berg PR, Davidson WS, Omholt SW, Kent MP: A dense SNP-based linkage map for Atlantic salmon (Salmo salar) reveals extended chromosome homeologies and striking differences in sex-specific recombination patterns. BMC Genomics. 2011, 12: 1-10.1186/1471-2164-12-1.View ArticleGoogle Scholar
- Gharbi K, Coulibaly I, Rexroad CE, Moghadam HK, Leder EH, Siemon H, Davidson WD, Guyomard R, Ferguson MM, Danzmann RG: Comparative maps of salmonid genomes: An update. Aquaculture. 2007, 272: S260-S261.View ArticleGoogle Scholar
- Lubieniecki KP, Jones SL, Davidson EA, Park J, Koop BF, Walker S, Davidson WS: Comparative genomic analysis of Atlantic salmon, Salmo salar, from Europe and North America. BMC Genet. 2010, 11: 105-PubMed CentralView ArticlePubMedGoogle Scholar
- Timusk ER, Ferguson MM, Moghadam HK, Norman JD, Wilson CC, Danzmann RG: Genome evolution in the fish family salmonidae: generation of a brook charr genetic map and comparisons among charrs (Arctic charr and brook charr) with rainbow trout. BMC Genet. 2011, 12: 68-PubMed CentralView ArticlePubMedGoogle Scholar
- Seeb JE, Carvalho G, Hauser L, Naish K, Roberts S, Seeb LW: Single-nucleotide polymorphism (SNP) discovery and applications of SNP genotyping in nonmodel organisms. Mol Ecol Resour. 2011, 11: 1-8.View ArticlePubMedGoogle Scholar
- Eggers DM, Irvine JR: Trends in abundance and biological characteristics for North Pacific sockeye salmon. North Pacific Anadromous Fish Commission Bulletin. 2007, 4: 53-75.Google Scholar
- Schindler DE, Hilborn R, Chasco B, Boatright CP, Quinn TP, Rogers LA, Webster MS: Population diversity and the portfolio effect in an exploited species. Nature. 2010, 465 (7298): 609-612. 10.1038/nature09060.View ArticlePubMedGoogle Scholar
- Irvine JR, Gross MR, Wood CC, Holtby LB, Schubert ND, Amiro PG: Canada’s Species at Risk Act: An opportunity to protect “endangered” salmon. Fisheries. 2005, 30 (12): 11-19. 10.1577/1548-8446(2005)30[11:CSARA]2.0.CO;2.View ArticleGoogle Scholar
- Ruckelshaus MH, Levin P, Johnson JB, Kareiva PM: The Pacific salmon wars: What science brings to the challenge of recovering species. Annu Rev Ecol Syst. 2002, 33: 665-706. 10.1146/annurev.ecolsys.33.010802.150504.View ArticleGoogle Scholar
- Eliason EJ, Clark TD, Hague MJ, Hanson LM, Gallagher ZS, Jeffries KM, Gale MK, Patterson DA, Hinch SG, Farrell AP: Differences in thermal tolerance among sockeye salmon populations. Science. 2011, 332 (6025): 109-112. 10.1126/science.1199158.View ArticlePubMedGoogle Scholar
- Bradford MJ, Lovy J, Patterson DA: Infection of gill and kidney of Fraser River sockeye salmon, Oncorhynchus nerka (Walbaum), by Parvicapsula minibicornis and its effect on host physiology. J Fish Dis. 2010, 33 (9): 769-779. 10.1111/j.1365-2761.2010.01178.x.View ArticlePubMedGoogle Scholar
- Bentley KT, Burgner RL: An assessment of parasite infestation rates of juvenile sockeye salmon after 50 years of climate warming in southwest Alaska. Environ Biol Fishes. 2011, 92 (2): 267-273. 10.1007/s10641-011-9830-2.View ArticleGoogle Scholar
- Miller KM, Li SR, Kaukinen KH, Ginther N, Hammill E, Curtis JMR, Patterson DA, Sierocinski T, Donnison L, Pavlidis P, et al: Genomic signatures predict migration and spawning failure in wild Canadian salmon. Science. 2011, 331 (6014): 214-217. 10.1126/science.1196901.View ArticlePubMedGoogle Scholar
- Jacob C, McDaniels T, Hinch S: Indigenous culture and adaptation to climate change: sockeye salmon and the St’at’imc people. Mitig Adapt Strat Glob Chang. 2010, 15 (8): 859-876. 10.1007/s11027-010-9244-z.View ArticleGoogle Scholar
- Ruff CP, Schindler DE, Armstrong JB, Bentley KT, Brooks GT, Holtgrieve GW, McGlauflin MT, Torgersen CE, Seeb JE: Temperature-associated population diversity in salmon confers benefits to mobile consumers. Ecology. 2011, 92 (11): 2073-2084. 10.1890/10-1762.1.View ArticlePubMedGoogle Scholar
- Hohenlohe PA, Bassham S, Etter PD, Stiffler N, Johnson EA, Cresko WA: Population genomics of parallel adaptation in threespine stickleback using sequenced RAD Tags. PLoS Genet. 2010, 6 (2): e1000862-10.1371/journal.pgen.1000862.PubMed CentralView ArticlePubMedGoogle Scholar
- Miller MR, Dunham JP, Amores A, Cresko WA, Johnson EA: Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers. Genome Res. 2007, 17 (2): 240-248. 10.1101/gr.5681207.PubMed CentralView ArticlePubMedGoogle Scholar
- Baird NA, Etter PD, Atwood TS, Currey MC, Shiver AL, Lewis ZA, Selker EU, Cresko WA, Johnson EA: Rapid SNP discovery and genetic mapping using sequenced RAD markers. PLoS One. 2008, 3 (10): e3376-10.1371/journal.pone.0003376.PubMed CentralView ArticlePubMedGoogle Scholar
- Etter PD, Preston JL, Bassham S, Cresko WA, Johnson EA: Local de novo assembly of RAD paired-end contigs using short sequencing reads. PLoS One. 2011, 6 (4): e18561-10.1371/journal.pone.0018561.PubMed CentralView ArticlePubMedGoogle Scholar
- Allendorf FW, Thorgaard GH: Tetraploidy and the evolution of salmonid fishes. Evolutionary Genetics of Fishes. Edited by: Turner BJ. 1984, New York: Plenum Press, 1-53.View ArticleGoogle Scholar
- Phillips R, Rab P: Chromosome evolution in the Salmonidae (Pisces): an update. Biol Rev. 2001, 76 (1): 1-25. 10.1017/S1464793100005613.View ArticlePubMedGoogle Scholar
- McClelland EK, Naish KA: A genetic linkage map for coho salmon (Oncorhynchus kisutch). Anim Genet. 2008, 39 (2): 169-179. 10.1111/j.1365-2052.2008.01699.x.View ArticlePubMedGoogle Scholar
- Gharbi K, Gautier A, Danzmann RG, Gharbi S, Sakamoto T, Hoyheim B, Taggart JB, Cairney M, Powell R, Krieg F, et al: A linkage map for brown trout (Salmo trutta): Chromosome homeologies and comparative genome organization with other salmonid fish. Genetics. 2006, 172 (4): 2405-2419.PubMed CentralView ArticlePubMedGoogle Scholar
- Woram RA, McGowan C, Stout JA, Gharbi K, Ferguson MM, Hoyheim B, Davidson EA, Davidson WS, Rexroad C, Danzmann RG: A genetic linkage map for Arctic char (Salvelinus alpinus): evidence for higher recombination rates and segregation distortion in hybrid versus pure strain mapping parents. Genome. 2004, 47 (2): 304-315. 10.1139/g03-127.View ArticlePubMedGoogle Scholar
- Greene BA, Seeb JE: SINE and transposon sequences generate high-resolution DNA fingerprints, ‘SINE prints’, that exhibit faithful Mendelian inheritance in pink salmon (Oncorhynchus gorbuscha). Mol Mar Biol Biotechnol. 1997, 6 (4): 328-338.Google Scholar
- Lindner KR, Seeb JE, Habicht C, Knudsen KL, Kretschmer E, Reedy DJ, Spruell P, Allendorf FW: Gene-centromere mapping of 312 loci in pink salmon by half-tetrad analysis. Genome. 2000, 43 (3): 538-549. 10.1139/g00-016.View ArticlePubMedGoogle Scholar
- Spruell P, Pilgrim KL, Greene BA, Habicht C, Knudsen KL, Lindner KR, Olsen JB, Sage GK, Seeb JE, Allendorf FW: Inheritance of nuclear DNA markers in gynogenetic haploid pink salmon. J Hered. 1999, 90 (2): 289-296. 10.1093/jhered/90.2.289.View ArticlePubMedGoogle Scholar
- Baxter SW, Davey JW, Johnston JS, Shelton AM, Heckel DG, Jiggins CD, Blaxter ML: Linkage mapping and comparative genomics using next-generation RAD sequencing of a non-model organism. PLoS One. 2011, 6 (4): e19315-10.1371/journal.pone.0019315.PubMed CentralView ArticlePubMedGoogle Scholar
- Chutimanitsakun Y, Nipper RW, Cuesta-Marcos A, Cistue L, Corey A, Filichkina T, Johnson EA, Hayes PM: Construction and application for QTL analysis of a Restriction Site Associated DNA (RAD) linkage map in barley. BMC Genomics. 2011, 12: 4-10.1186/1471-2164-12-4.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips RB, Keatley KA, Morasch MR, Ventura AB, Lubieniecki KP, Koop BF, Danzmann RG, Davidson WS: Assignment of Atlantic salmon (Salmo salar) linkage groups to specific chromosomes: Conservation of large syntenic blocks corresponding to whole chromosome arms in rainbow trout (Oncorhynchus mykiss). BMC Genet. 2009, 10: 46-PubMed CentralView ArticlePubMedGoogle Scholar
- Gilbey J, Verspoor E, McLay A, Houlihan D: A microsatellite linkage map for Atlantic salmon (Salmo salar). Anim Genet. 2004, 35 (2): 98-105. 10.1111/j.1365-2052.2004.01091.x.View ArticlePubMedGoogle Scholar
- Moen T, Hayes B, Baranski M, Berg PR, Kjoglum S, Koop BF, Davidson WS, Omholt SW, Lien S: A linkage map of the Atlantic salmon (Salmo salar) based on EST-derived SNP markers. BMC Genomics. 2008, 9: 223-10.1186/1471-2164-9-223.PubMed CentralView ArticlePubMedGoogle Scholar
- Moen T, Hoyheim B, Munck H, Gomez-Raya L: A linkage map of Atlantic salmon (Salmo salar) reveals an uncommonly large difference in recombination rate between the sexes. Anim Genet. 2004, 35 (2): 81-92. 10.1111/j.1365-2052.2004.01097.x.View ArticlePubMedGoogle Scholar
- Alfaqih MA, Phillips RB, Wheeler PA, Thorgaard GH: The cutthroat trout Y chromosome is conserved with that of rainbow trout. Cytogenet Genome Res. 2008, 121 (3–4): 255-259.View ArticlePubMedGoogle Scholar
- Wu RL, Ma CX, Painter I, Zeng ZB: Simultaneous maximum likelihood estimation of linkage and linkage phases in outcrossing species. Theor Popul Biol. 2002, 61 (3): 349-363. 10.1006/tpbi.2002.1577.View ArticlePubMedGoogle Scholar
- Palti Y, Luo MC, Hu YQ, Genet C, You FM, Vallejo RL, Thorgaard GH, Wheeler PA, Rexroad CE: A first generation BAC-based physical map of the rainbow trout genome. BMC Genomics. 2009, 10: 462-10.1186/1471-2164-10-462.PubMed CentralView ArticlePubMedGoogle Scholar
- Slate J: Robustness of linkage maps in natural populations: a simulation study. Proceedings of the Royal Society B-Biological Sciences. 2008, 275 (1635): 695-702. 10.1098/rspb.2007.0948.PubMed CentralView ArticleGoogle Scholar
- Nielsen R, Paul JS, Albrechtsen A, Song YS: Genotype and SNP calling from next-generation sequencing data. Nat Rev Genet. 2011, 12 (6): 443-451. 10.1038/nrg2986.PubMed CentralView ArticlePubMedGoogle Scholar
- Danzmann RG, Davidson EA, Ferguson MM, Gharbi K, Koop BF, Hoyheim B, Lien S, Lubieniecki KP, Moghadam HK, Park J, et al: Distribution of ancestral proto-Actinopterygian chromosome arms within the genomes of 4R-derivative salmonid fishes (Rainbow trout and Atlantic salmon). BMC Genomics. 2008, 9:Google Scholar
- Faber-Hammond J, Phillips RB, Park LK: The sockeye salmon neo-Y chromosome is a fusion between linkage groups orthologous to the coho Y chromosome and the long arm of rainbow trout chromosome 2. Cytogenet Genome Res. 2012, 136 (1): 69-74. 10.1159/000334583.View ArticlePubMedGoogle Scholar
- Kidwell MG: Transposable elements and the evolution of genome size in eukaryotes. Genetica. 2002, 115 (1): 49-63. 10.1023/A:1016072014259.View ArticlePubMedGoogle Scholar
- De Boer JG, Yazawa R, Davidson WS, Koop BF: Bursts and horizontal evolution of DNA transposons in the speciation of pseudotetraploid salmonids. BMC Genomics. 2007, 8: 422-10.1186/1471-2164-8-422.PubMed CentralView ArticlePubMedGoogle Scholar
- Habicht C, Seeb LW, Myers KW, Farley EV, Seeb JE: Summer-fall distribution of stocks of immature sockeye salmon in the Bering Sea as revealed by single-nucleotide polymorphisms (SNPs). Trans Am Fish Soc. 2010, 139: 1171-1191. 10.1577/T09-149.1.View ArticleGoogle Scholar
- McGlauflin MT, Schindler DE, Seeb LW, Smith CT, Habicht C, Seeb JE: Spawning habitat and geography influence population structure and juvenile migration timing of sockeye salmon in the Wood River Lakes, Alaska. Trans Am Fish Soc. 2011, 140 (3): 763-782. 10.1080/00028487.2011.584495.View ArticleGoogle Scholar
- Gomez-Uchida D, Seeb JE, Smith MJ, Habicht C, Quinn TP, Seeb LW: Single nucleotide polymorphisms unravel hierarchical divergence and signatures of selection among Alaskan sockeye salmon (Oncorhynchus nerka) populations. BMC Evol Biol. 2011, 11 (1): 48-10.1186/1471-2148-11-48.PubMed CentralView ArticlePubMedGoogle Scholar
- Everett MV, Grau ED, Seeb JE: Short reads and nonmodel species: exploring the complexities of next-generation sequence assembly and SNP discovery in the absence of a reference genome. Mol Ecol Resour. 2011, 11: 93-108.View ArticlePubMedGoogle Scholar
- Andersen EC, Gerke JP, Shapiro JA, Crissman JR, Ghosh R, Bloom JS, Felix M-A, Kruglyak L: Chromosome-scale selective sweeps shape Caenorhabditis elegans genomic diversity. Nat Genet. 2012, advance online publicationGoogle Scholar
- Elfstrom CM, Smith CT, Seeb JE: Thirty-two single nucleotide polymorphism markers for high-throughput genotyping of sockeye salmon. Molecular Ecology Notes. 2006, 6 (4): 1255-1259. 10.1111/j.1471-8286.2006.01507.x.View ArticleGoogle Scholar
- Smith CT, Elfstrom CM, Seeb LW, Seeb JE: Use of sequence data from rainbow trout and Atlantic salmon for SNP detection in Pacific salmon. Mol Ecol. 2005, 14 (13): 4193-4203. 10.1111/j.1365-294X.2005.02731.x.View ArticlePubMedGoogle Scholar
- Campbell NR, Narum SR: Development of 54 novel single-nucleotide polymorphism (SNP) assays for sockeye and coho salmon and assessment of available SNPs to differentiate stocks within the Columbia River. Mol Ecol Resour. 2011, 11: 20-30.View ArticlePubMedGoogle Scholar
- Seeb JE, Pascal CE, Ramakrishnan R, Seeb LW: SNP genotyping by the 5’-nuclease reaction: advances in high throughput genotyping with non-model organisms. Methods in Molecular Biology, Single Nucleotide Polymorphisms. Edited by: Komar A. 2009, New York: Humana Press, 2Google Scholar
- Langmead B, Trapnell C, Pop M, Salzberg SL: Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009, 10 (3): R25-10.1186/gb-2009-10-3-r25.PubMed CentralView ArticlePubMedGoogle Scholar
- Garcia AAF, Margarido GRA, Souza AP: OneMap: software for genetic mapping in outcrossing species. Hereditas. 2007, 144 (3): 78-79. 10.1111/j.2007.0018-0661.02000.x.View ArticlePubMedGoogle Scholar
- Kosambi DD: The estimation of map distances from recombination values. Ann Eugen. 1943, 12: 172-175. 10.1111/j.1469-1809.1943.tb02321.x.View ArticleGoogle Scholar
- Thorgaard GH, Nichols KM, Young WP, Danzmann RG, Robison BD, Rexroad C, Noakes M, Phillips RB, Bentzen P, Spies I, et al: A consolidated linkage map for rainbow trout (Oncorhynchus mykiss). Anim Genet. 2003, 34 (2): 102-115. 10.1046/j.1365-2052.2003.00957.x.View ArticlePubMedGoogle Scholar
- Danzmann RG, Gharbi K: Gene mapping in fishes: a means to an end. Genetica. 2001, 111 (1–3): 3-23.View ArticlePubMedGoogle Scholar
- Zerbino DR, Birney E: Velvet: Algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008, 18 (5): 821-829. 10.1101/gr.074492.107.PubMed CentralView ArticlePubMedGoogle Scholar