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Table 1 Mapping parameters of parental linkage maps constructed by merging two reciprocal crosses: C14xC15 and C15xC14

From: Annotated genetic linkage maps of Pinus pinaster Ait. from a Central Spain population using microsatellite and gene based markers

Mapping parameter

C14

C15

Total number of available markers

402

410

 Number of SSRs loci

11

13

 Number of ESTP loci a

13

12

 Number of SAMPL loci

228

237

 Number of SNP loci b

150

148

Total number of distorted (p ≤ 0.01) markers

39

52

Number of excluded markers c

62

72

 Number of SSRs loci

1

1

 Number of ESTP loci

0

0

 Number of SAMPL loci

46

60

 Number of SNP loci

15

11

Number of markers not excluded

340

338

Number of assigned markers d

321

319

 Number of SSRs loci

9

10

 Number of ESTP loci

11

11

 Number of SAMPL loci

174

166

 Number of SNP loci

127

132

Number of positioned markers e

215 (63.2%)

211 (62.4%)

 Number of SSR loci

6 (60%)

7 (58.3%)

 Number of ESTP loci

10 (76.9%)

7 (58.3%)

 Number of SAMPL loci

98 (53.8%)

98 (55.4%)

 Number of SNP loci

101 (74.8%)

99 (72.3%)

Number of distorted (p ≤ 0.01) positioned markers

14

14

Unlinked markers (%) f

19(6.3%)

19 (5.6%)

Number of LG >3 before making alignments

22

20

Number of LG >3 after making alignments

13

14

Smallest LG (cM) before making alignments

17.5

13.4

Largest LG (cM) before making alignments

81.1

155.3

Average length (cM) LG ± SD before alignments

53.7 ± 20.6

69 ± 35.6

Smallest LG (cM) after making alignments

52

42.3

Largest LG (cM) after making alignments

142.2

155.3

Average length (cM) of a LG ± SD after alignments

90.8 ± 29.14

98.5 ± 38

Maximum distance (cM) between 2 adjacent markers

24.9

35.3

Average distance (cM) between 2 adjacent markers ± SD

6.12 ± 5.8

7.22 ± 6.4

Observed map length (cM)

1180.4

1379.5

Estimated map length (cM)

1870.2

2166.6

Observed map coverage

63%

64%

  1. a The 25 ESTP-s correspond to 11 gene loci.
  2. b The SNPs markers correspond to 47 gene loci and 143 contigs.
  3. c Markers with more than 70% of missing data (see Methods section) and identical markers.
  4. d Assigned markers correspond to markers linked with more than 2 other markers.
  5. e Unpositioned markers correspond to markers with a recombination frequency higher than 0.35 with the nearest linked marker (unlinked markers) or markers which position could not be reliably estimated. Percentage of positioned markers was calculated over the number of not excluded markers.
  6. f Percentage of unlinked markers was calculated over the number of not excluded markers.
  7. SD Standard deviation.