Figure 3

dTTP biosynthesis and TMPK. a. Cellular de novo pathway of dTTP biosynthesis. Enzymes present in EpapGV and other baculoviruses are marked with an asterisk (*). TMPK gene present in EpapGV and absent in other baculoviruses is highlighted with two asterisks (**). Solid arrows correspond to the de novo pathway of dTTP biosynthesis and the dashed arrow to the salvage pathway b. Alignment of EpapGV TMPK with other TMPKs from several organisms (only the genus is indicated) and viruses: Anopheles gambiae (XP_314179.3); Arabidopsis thaliana (NP_001078772.1); Sorghum bicolor (XP_002461104.1); Zea mays (NP_001150303.1); Drosophila ananassae (XP_001960115.1); Dictyostelium discoideum (XP_635930.1); Saccharomyces cerevisiae (NP_012591.1); Tetrahymena thermophila (XP_001009062.1); Yarrowia lipolytica (XP_501790.1); II6 (NP_149714.1); Variola Major Virus (NP_042204.1); CyHV3 (YP_001096175.1); ASFV (NP_042729.1); Vaccinia Virus (AAW23610.1); WSSV (AAG40728.1); Heliconius melpomene (CBH09285.1). Identical residues conserved in all the sequences are shaded in yellow; those conserved in most sequences are shaded in light blue, and conservative changes are shaded in green.