Table 2 Comparison of assembly of pepper Sanger ESTs versus assembly of IGA reads
Statistics | EST Assembly | Transcriptome Assembly |
|---|---|---|
Number of Unigenes | 31,196 | 123,261 |
Total assembled nucleotides | 21,665,127 | 135,019,787 |
Average GC content (%) | 41 | 39 |
Longest Contig Length | 3,989 | 19,089 |
Average Contig Length | 694.5 | 1,095 |
Median Contig Length | 651 | 697 |
N50 | 702 | 1,647 |
Number/Percent Contigs Size < 1 KB | 27,248 | 78,433 |
   1–2 KB | 3,634 | 27,436 |
   2–3 KB | 288 | 10,616 |
   3–4 KB | 26 | 3,955 |
   4–5 KB | 0 | 1,559 |
   5–10 KB | 0 | 1,184 |
   10–20 KB | 0 | 78 |