Figure 3

2D gel electrophoretic separation of protein extracts of A. fumigatus grown under normoxic and hypoxic growth conditions. In total, 86 different proteins of A. fumigatus changed significantly their abundance within the first 24 hours of hypoxia (protein spots are labeled with spot numbers as indicated in Additional file 3 and 5). A. fumigatus proteins were labeled with the CyDye DIGE Fluor minimal dye labeling kit. Subsequently, proteins were separated by 2D gel electrophoresis using immobilized pH gradient strips with a pH range of (A) 3-7 NL and (B) 7-11 NL in the first dimension. For the separation of proteins in the second dimension, SDS-polyacrylamide gradients gels (11-16%) were used. Differentially regulated proteins were identified by MALDI-TOF/TOF analysis. A three color overlaid gel image is shown. Samples were labeled as follows: 0 hour control sample (Cy5), 24 hour hypoxia sample (Cy3) and internal standard (Cy2).