Unusual domain architecture of aminoacyl tRNA synthetases and their paralogs from Leishmania major
© Gowri et al.; licensee BioMed Central Ltd. 2012
Received: 11 April 2012
Accepted: 30 October 2012
Published: 14 November 2012
Leishmania major, a protozoan parasite, is the causative agent of cutaneous leishmaniasis. Due to the development of resistance against the currently available anti-leishmanial drugs, there is a growing need for specific inhibitors and novel drug targets. In this regards, aminoacyl tRNA synthetases, the linchpins of protein synthesis, have received recent attention among the kinetoplastid research community. This is the first comprehensive survey of the aminoacyl tRNA synthetases, their paralogs and other associated proteins from L. major.
A total of 26 aminoacyl tRNA synthetases were identified using various computational and bioinformatics tools. Phylogenetic analysis and domain architectures of the L. major aminoacyl tRNA synthetases suggest a probable archaeal/eukaryotic origin. Presence of additional domains or N- or C-terminal extensions in 11 aminoacyl tRNA synthetases from L. major suggests possibilities such as additional tRNA binding or oligomerization or editing activity. Five freestanding editing domains were identified in L. major. Domain assignment revealed a novel asparagine tRNA synthetase paralog, asparagine synthetase A which has been so far reported from prokaryotes and archaea.
A comprehensive bioinformatic analysis revealed 26 aminoacyl tRNA synthetases and five freestanding editing domains in L. major. Identification of two EMAP (endothelial monocyte-activating polypeptide) II-like proteins similar to human EMAP II-like proteins suggests their participation in multisynthetase complex formation. While the phylogeny of tRNA synthetases suggests a probable archaeal/eukaryotic origin, phylogeny of asparagine synthetase A strongly suggests a bacterial origin. The unique features identified in this work provide rationale for designing inhibitors against parasite aminoacyl tRNA synthetases and their paralogs.
KeywordsAminoacyl tRNA synthetases Paralog Editing domains
Aminoacyl tRNA synthetases (aaRSs) are indispensable components of protein synthesis. They covalently append amino acids to their cognate tRNA. Most organisms possess separate tRNA synthetases for each of the 20 standard amino acids. There are two classes of aminoacyl tRNA synthetases each comprising of ~10 tRNA synthetase enzymes - Class I tRNA synthetases contain the classical Rossmann-nucleotide binding catalytic domain with two highly conserved 'HIGH' and 'KMSKS' catalytic motifs which are critical for their function . Class II enzymes contain a central antiparallel β-sheet flanked by α-helices. Despite these structural and sequence differences, both tRNA synthetases catalyse the same two step reaction. The first step involves activation of the amino acid by ATP to form aminoacyl adenylate. The second step is the attachment of the aminoacyl group to the cognate tRNA. While Class I attaches aminoacyl group to the 2'-hydroxyl group of tRNA, Class II synthetases attach them to 3'-hydroxylgroup of tRNA. The 3-D structure and the specific anticodon in the tRNA determine the specificity of tRNA synthetases. Most eukaryotes carry two genes for each of the 20 standard aminoacyl tRNA synthetases (cytosolic and mitochondrial). However, trypanosomatids carry only a single copy per aminoacid except for Asp, Trp and Lys [2, 3].
Gene knock out studies of the Trypanosomal histidyl tRNA synthetase showed a complete arrest of growth in the bloodstream forms of the parasite suggesting an essential role in cell survival . Mammalian methionyl tRNA synthetase provides a cytosolic anchoring site for Aminoacyl tRNA synthetase Interaction Multifunctional Protein-3 (AIMP-3/p18), a potent tumor suppressor in addition to its essential role in initiating translation . Participation of aminoacyl tRNA synthetases in cell apoptosis, rRNA synthesis, RNA trafficking, multisynthetase enzyme complex formation supplement their essential role in protein synthesis [6, 7]. As an inevitable component of protein synthesis, aminoacyl tRNA synthetases have been important antibacterial drug targets. An important example of aaRS inhibitor is provided by the antibiotic mupirocin which selectively inactivates bacterial Isoleucyl-tRNA synthetase (IleRS) [8, 9]. Four distinct aminoacyl tRNA synthetases in Anabaena sp. PCC 7120 contain a novel CAAD domain that bears putative transmembrane helices. Domain deletion studies indicate its essential role in membrane anchoring and a purely structural role and do not alter the catalytic properties of the enzyme . Eukaryotic tRNA synthetases, unlike their prokaryotic counterparts carry additional domains or extensions in their N- or C-terminal regions which mediate protein-protein interaction or involved in tRNA binding. A comprehensive computational analysis of the aminoacyl tRNA synthetases of Plasmodium falciparum reveals novel domain architectures. Phylogenetic analyses of several Pf aaRSs reconcile their evolutionary link to plants and bacteria . Recently, the expression and localization profiles of the cis- and trans- aaRS editing domains of P. falciparum showed an uneven distribution of 8 aaRS editing domains in the different cellular compartments .
Leishmaniasis is one of the deadly diseases caused by the different species of Leishmania. Increasing resistance to presently available anti-leishmanial drugs poses a need for identification of novel drug targets as well as specific inhibitors for treating leishmaniasis. In this regard, aminoacyl tRNA synthetases, the versatile players of protein translation machinery have received attention in kinetoplastid research community . Very recently, crystal structure of a methionyl-tRNA synthetase  and a novel pseudodimeric structure of a tyrosyl tRNA synthetase from Leishmania major have been solved . Substantial differences between the human tRNA synthetases and the L. major tRNA synthetase homologue promise a rationale for designing inhibitors to selectively target the parasite enzyme. A comprehensive bioinformatic analysis employing the profile-based hidden markov model (HMM) has identified aaRSs and aaRS related proteins from L. major. The sequence features and novel domain architectures of aaRSs from L. major were analyzed using a combination of BLAST and HMM search tools. Domain assignment revealed a novel asparagine tRNA synthetase (AsnRS) paralog Asparagine synthetase A (AsnA) which has been so far reported from prokaryotes and archaea and has been shown to be absent in eukaryotes. We for the first time report the phylogeny and structural analysis of a eukaryotic AsnA from L. major.
Results and discussions
List of all the aminoacyl tRNA synthetases and their associated proteins, aaRS paralogs and editing domains with their CDD domain assignments and subcellular localization
HMM based function assignment
Swissprot function assignment
Subcellular location (PSORT II)
CDD based domain assignment
CLASS I aaRS
GlnRS_core; GlnRS_core; tRNA_synt_Ic_C_superfamily
GlnRS_core; GlnRS_core; tRNA_synt_Ic_C_superfamily
Arg_tRNA_synt_N; ArgRS_Core; anticodon_Ia_like_superfamily
ValRS_core; anticodon_Ia_Val; Val_tRNA_synt_C_superfamily
CLASS II aaRS
AspRS_cyto_N; ClassII_aaRS_like_core_superfamily; ClassII_aaRS_like_core_superfamily
tRNA_synt_Ic_R1_superfamily; AsnRS_cyto_like_N; AsxRS_core
B3_4_superfamily; B5_superfamily; PheRS_beta_core
Ybak_like_superfamily; ProRS_arch_euk; HGTP_anticodon_superfamily
Ybak_like_superfamily; ProRS_arch_euk; HGTP_anticodon_superfamily
TGS_superfamily; tRNA_SAD; ThrRS_Core; HGTP_anticodon
O-Phosphoseryl-tRNA Selenium transferase
SLA/LP autoantigen-like protein
aaRS associated proteins
Asparagine Synthetase A
Aspartate, ammonia ligase
Trans Editing domains
As the key players in protein translation, most organisms require 20 standard aminoacyl tRNA synthetases for protein synthesis. However, indirect routes of GlntRNAGln and AsntRNAAsn synthesis also exist in many organisms which either completely lack the respective tRNA synthetases or lack them in some specific organelles such as mitochondria . Kinetplastid (Trypanosoma and Leishmania) Seryl tRNA synthetases (SerRS) show a close functional and evolutionary relationship to the metazoan SerRS which is supported by the presence of a metazoan-trypanosomatid specific sequence insertion in SerRS . The kinetoplastid SerRS also show high affinity for tRNASec. Proteins containing non-standard aminoacids such as Sec (Selenocysteine) have been reported from trypanosomatids . The presence of Selenocysteine incorporation is further supported by the presence of a selenophosphosynthetase (LmjF.36.5410), the first enzyme in the selenocysteine tRNA synthesis as well as a selenocysteine specific elongation factor (LmjF.34.2840). LmjF.09.0950, a o-phosphoseryl tRNA(sec) selenium transferase, the third enzyme in the SectRNASec synthesis with SepSecS-like domains was also identified using the Hidden Markov Model searches.
The tRNA synthetases and other associated proteins such as the EMAP II and the editing domains although present in all the trypanosomatids, show interesting differences between the Leishmania and Trypanosoma [Additional file 2: Table S2]. There are two AspRS in all the Leishmania Spp, T. brucei brucei and T. brucei gambiense. However, T. congolense and T. vivax have only a single copy of AspRS. Furthermore, all the Leishmania spp and Trypanosoma spp carry a single HisRS except T. congolense. T. cruzi Non-Esmeraldo strain and T. congolense carry three TrpRS whereas all other trypanostomatids carry two TrpRS. T. cruzi Non-Esmeraldo strain lacks a MetRS and GlnRS. Moreover, T. cruzi Non-Esmeraldo strain contains only an alpha chain of PheRS and T. cruzi Esmeraldo strain lacks an AsnRS and ArgRS. Although several tRNA synthetases are syntenic and conserved in Leishmania, the protein expression is regulated at different stages in different Leishmania species. For example, SerRS (LmjF.11.0100), LysRS (LmjF.15.230), glutamyl (LmjF.30.3240), AsnRS (LmjF.34.2340), GlyRS (LmjF.36.3840), IleRS (LmjF.36.5620) are regulated predominantly in the promastigotes in L. major.
Domain architecture of tRNA synthetases from L. major
Alanyl and threonyl tRNA synthetases often possess a secondary associated domain (tRNA_SAD) containing a HxxxH motif which is typical of a metal dependent hydrolases . Alanyl (LmjF.22.1540) and threonyl tRNA synthetases (LmjF.35.1410) of L. major contain this domain. The presence of a tRNA_SAD domain with a conserved HxxxH motif suggests a functionally important hydrolytic activity (Figure 2b). Lm ThrRS also contains a TGS domain tethered N-terminus to the tRNA_SAD (Figure 2b). Based on its occurrence in other regulatory proteins, this domain is proposed to bind ligands (most likely nucleotides) . Hence, the TGS domain in Lm ThrRS probably has a regulatory role. In addition to an editing domain tRNA_SAD, Lm AlaRS has a C-terminal extension (DHHA1 domain). Crystal structure and functional analysis of this C–Ala extension in A. aeolicus AlaRS shows that it promotes cooperative binding of the aminoacylation and editing domain to tRNAAla.
While the C-terminal extension of Lm AlaRS might be involved in oligomerization, N- or C-terminal extensions of SerRS, LeuRS and LysRS have been shown to provide additional tRNA binding to these synthetases [29–31]. The N-terminal extension of Lm LeuRS (LmjF.13.1100) is present as insertion in the editing domain denoted as CP1 (Connective Polypeptide) (Figure 2a). Only three LeuRS editing domains have been structurally characterized till date [31, 32]. The CP1 of E. coli LeuRS lacks this insertion and hence lacks the editing activity as an isolated CP1 domain . Recent crystallographic and biochemical evidences reconcile this observation . Lm LeuRS has an N-terminal extension of approximately 35 residues long. Secondary structure prediction of this insertion using PSIPRED server  suggests that this N-terminal extension has a helix of ~15 residues long (Additional file 3: Figure S1). Further, sequence comparison of the editing domain of Lm leuRS with that of human, E. coli, A. aeolicus and G. lamblia suggest the T-rich region, GTG motif and the conserved Asp essential for function are all conserved. Sequence-based phylogeny suggests a close evolutionary relationship of Lm LeuRSCP1 to Gl LeuRSCP1 which has been verified to possess fully functional editing domain in isolation . The antifungal drug (AN2690) binding residues of C. albicans LeuRS are also highly conserved in Lm LeuRS editing domain. Although Lm LeuRS is ~1100 residues long, the presence of a probable functional editing domain in isolation proves it to be a novel drug target and encourages experimental verification for its drug binding abilities.
L. major encodes two cytosolic LysRS (LmjF.15.0230 and LmjF.30.0130) (Table 1). One of the Lm LysRS (LmjF.15.0230) has an N-terminal extension (DUF972) similar to the mammalian LysRS (Figure 2b). The N-terminal extension of mammalian LysRS has been shown to participate in non-specific tRNA binding . Deletion of this N-terminal extension has been shown to reduce the tRNA binding affinity by 100-fold and hence decreases the aminoacylation of tRNAlys by 3-fold in mammals . Based on the sequence homology of the Lm LysRS (LmjF.15.0230) to the mammalian LysRS, the N-terminal extension in LmjF.15.0230 can be expected to participate in a non-specific tRNA binding and could probably play a role in amino acylation activity of this LysRS. LmjF.15.0230 also contains an ‘ELR’ motif at the N-terminal extension. However, chemokine activity of this ‘ELR’ motif in Lm LysRS requires experimental verification.
Stand-alone deacylase/trans editing domains in L. major
While the pairing of the correct amino acid to their cognate tRNA is done by the aminoacyl tRNA synthetases, faithful translation in protein synthesis is ensured by the presence of editing domains(ED) either tethered to the aaRS (cis editing domains) or as free standing editing domains (trans editing domains). There are 8 cis editing domains tethered to AlaRS, ThrRS, PheRS, LeuRS, IleRS, ValRS, ProRS (both the copies) and 5 trans editing domains which includes the AlaX (AlaRS ED), two YbaK-like (ProRS ED) and two D-tyrosyl deacylases (DTDAs) in L. major.
The Second Associated domain (tRNA_SAD) of AlaRS/ThrRS are generally tethered to the synthetase core. In L. major, in addition to the tethered editing domains in the AlaRS (LmjF.22.1540) and ThrRS (LmjF.35.1410), a freestanding tRNA_SAD (LmjF.15.0690) domain (Figure 2a) was also found. There are two types of standalone AlaX domains: AlaX-M, AlaX-S. Both the domains differ in their metal coordination types (Zn coordination). In AlaX-M, in addition to coordination with the Cysteine residues, there is coordination with a water molecule. However, in AlaX-S, the metal ion is coordinated only by cysteines [34, 35]. The standalone domain has all the four cysteines conserved. Sequence based phylogeny suggests that L. major tRNA_SAD standalone domain is closer to AlaX-M family (Additional file 4: Figure S2).
In addition to the tethered Ybak/ProX deacylase domains in the two Lm ProRS copies, two freestanding YbaK domains (LmjF.03.0710 and LmjF.21.0910) are also found (Figure 2a). Sequence based phylogeny of both tethered and standalone deacylase domains in L. major with the available crystal structures of YbaK/ProX domains suggest that the tethered deacylase domains are closer in terms of their amino acid sequence to ProX type which specifically deacylate the misacylated tRNAPro with Alanine [36, 37] while the trans deacylase domains are closer to YbaK-like which deacylate the misacylated tRNAPro with Cysteine [37, 38] (Additional file 5: Figure S3). The trans editing domain LmjF.03.0710 lacks the active site lysine which is shown to be critical in H. influenza YbaK domain by mutagenesis (K46 in PDB: 1DBX) [38–40]. Thus, only one of the trans editing domain of Lm ProRS (LmjF.21.0910) which has the active site lysine conserved might be functional.
Detection of D-amino acids in the form of free aminoacids, peptides and proteins in various living organisms from bacteria to human challenges the current concept of protein synthesis [41, 42]. D-Tyr tRNATyr deacylases (DTDA), a new class of tRNA dependent hydrolases provide a novel checkpoint by recycling the misaminoacylated D-Tyr tRNATyr. There are two DTDAs in L. major. One of the Lm DTDA2 (LmjF.34.3360) has a crystal structure solved using the Structural Genomics of Pathogenic Protozoa Consortium (SGPP) (PDB: 1TC5). This sequence is closer to the human, mouse DTDA2 homologue. The other Lm DTDA1 homologue (LmjF.36.2730) is closer to the Pf DTDA homologue (Additional file 6: Figure S4) whose structure has been solved recently (3KO5). Like other deacylases/editing domains of aaRSs, DTDA has been shown to be a novel drug target in P. falciparum. While structural comparison of the Lm DTDA2 and Pf DTDA1 shows major differences in the length of a specific loop, 3-D structural modeling of Lm DTDA1 homologue (LmjF.36.2730) based on the Pf DTD template structure and a comparative structural analysis of Lm DTDA1 with a perspective of inhibitor design is commendable.
Novel aminoacyl tRNA synthetases from L. major
Asparagine synthetase A from L. major – A novel enzyme specific to Prokaryotes and Archaea
AsnA genes have been reported from prokaryotes and archaea [59, 60], while AsnB genes are reported from all three domains of life. Leishmania and trypanosoma albeit being eukaryotes surprisingly possess AsnA (LmjF.26.0830) and AsnB (LmjF.29.1490). Swissprot data [18, 19] suggests the presence of AsnA in almost 368 organisms all belonging to prokaryotic origin. In addition to kinetoplastids, blast searches against EupathDB database  suggest parasites such as Trichomonas vaginalis (TVAG_340510; E-value: 8.9E-50); Entamoeba histolytica (EHI_148470; E-value: 1.4E-73); Cryptosporodium hominis (Chro.50501; E-value: 6.0E-26); Cryptosporodium parvum (cgd5_4540; E-value: 2.1E-52) possess a copy of AsnA gene. Lm AsnA is predicted to be a mitochondrial copy (Table 1). Among the Class II synthetases, lysine, asparagine and aspartic acid are closely related in their structure and belong to the same subtype (Class 2b) . However, evidences support the evolutionary link between the asparagine synthetase and aspartyl tRNA synthetases as they both recognize aspartic acid and ATP . Thus, a LysRS rooted sequence based phylogeny of AsnA along with AspRS and AsnRS catalytic core from all three domains of life clearly shows that the kinetoplastid and other eukaryotic pathogen AsnA enzymes are of bacterial origin (Figure 5b) while the archaeal AsnA is derived from gene duplication events from the ancestral AspRS as previously mentioned by Blaise and workers . Structure dependent sequence based phylogeny of all the available crystal structures of AspRS, AsnRS with the Ec AsnA and Pa AsnA enzymes show a similar tree branching with the Ec AsnA structurally closer to the bacterial AspRS (Figure 5c). The distinct branching of yeast AspRS free form clearly reflects the conformational rearrangements upon tRNA binding to the yeast AspRS .
To date crystal structures of three AsnA enzymes (from E. coli, P.abyssi and an AsnA peptide structure from P. furious) are available. Among the three, amino acid sequences of L. major AsnA and Ec AsnA closer to each other. Hence, a structural model of LmA snA was built using Ec AsnA (PDB:11AS) as the template using Modeller v 9.0. The model was energy minimized using Amber96 forcefield in gromacs. The quality of the energy minimized model is then verified using PROCHECK available at PDBSUM  webserver. Structural comparison of the Lm AsnA model with the E. coli and P.abyssi homologue suggests that the Lm AsnA shares 58% sequence identity with the bacterial homologue and 19% sequence identity with the archaeal homologue. The L. major model superposes with the E. coli and P. abyssi structures at 0.5Å and 2.9Å RMSD values respectively. 3-D structural comparison of the Lm AsnA model with the Ec AsnA (PDB:11AS) shows a complete conservation of catalytic residues, ATP binding Glycine rich region and identical flipping loop lengths that covers the active site at the sequence level and in 3-Dimension (Figure 5d). Based on the sequence and structural similarities between the E. coli and the L. major enzymes, D222-Q118 pair of Lm AsnA can be expected to anchor the beta carboxylate group of the L-aspartic acid. In yeast AspRS, these residues are substituted by a threonine and a glycine respectively and an Asp342 and Q303 which are at structurally different positions anchors the beta carboxylate group of the substrate . While in Lm AsnA, Ec AsnA and yeast AspRS, an Asp-Gln pair anchors the beta carboxylate group of L-aspartic acid, in archaeal AsnA and AspRS homologues; Aspartic acid is anchored by two arginines . This suggests that the altered substrate specificity and the reaction chemistry between the AspRS and AsnA have been achieved by a few residue substitutions at the active site. The basic difference in the substrate anchoring residues between the archaeal and bacterial/kinetoplastid AsnA enzymes suggests a distinct evolutionary origin between the archaeal and bacterial/kinetoplastid AsnA. These key differences between the archaeal and the kinetoplastid AsnA substrate recognition modes and the absence of AsnA in human make the kinetoplastid enzyme unique drug target for antiparasitic drug design.
Aminoacyl tRNA synthetases are ubiquitous enzymes essential for cell viability. Hence, they have been one of the promising drug targets in antimicrobial infections. Due to increasing resistance to currently available anti-leishmanial drugs, aminoacyl tRNA synthetases have received attention among the kinetoplastid research community in the recent times. In this study, aminoacyl tRNA synthetases and their associated proteins from L. major have been explored for their novel domain architectures and sequence features. Based on the domain architecture, we identified 26 indisputable aminoacyl tRNA synthetases from L. major, with a predominant predicted localisation of them in the cell cytosol. Sequence based phylogeny of some specific tRNA synthetases (AspRS and ProRS) confirm their close evolutionary relationship with archaeal/eukaryotic tRNA synthetases. In addition to the appended editing domains and N- or C-terminal extensions which provide additional tRNA binding, we also identified free standing editing domains of AlaRS/ThrRS, two ProRS deacylases and two D-tyrosine deacylases (DTD). Two novel EMAP II-like sequences containing a heptapeptide motif similar to the human EMAP II-like sequences were also identified. The presence of such EMAP II-like sequences suggests the formation of a probable multisynthetase protein complex as seen in the case of human or their probable role in trans-activation of certain aminoacyl tRNA synthetases. Presence of ‘ELR’ motif in Lys, Asn, Cys and Tyr tRNA synthetases provides clues for their participation in angiogenesis likely. We also highlight the sequence analysis and 3-D structural modelling of a unique enzyme that is completely absent in human, Asparagine synthetase A from L. major for the first time. While the aminoacyl tRNA synthetases of L. major show archaeal/eukaryotic origin, Asparagine synthetase A of L. major shows bacterial origin. The different substrate recognition modes of the baterial and archaeal enzymes makes them unique and worth exploring.
Leishmania major (Version 3.1) from TritrypDB database  is used here. Hidden Markov Models (HMM)  were generated using aminoacyl tRNA synthetase sequences and the editing domain sequences (Ybak, DTDA, AlaX) from Swissprot database Release 4.0, 2011 [67, 68] for each of the 21 tRNA synthetases (20 standard tRNA synthetases + o -phosphoseryl tRNA(sec) selenium transferase) and the deacylases of ProRS (YbaK), AlaRS (AlaX) and D-tyrosine deacylase (DTDA). The distribution of aaRS sequences from the individual domains of life used for the generation of HMMs is given in the Additional file 7: Table S3. hmmbuild and hmmsearch options in the suite of HMMER 3.0 package  was used for generation and searches using the HMMs respectively. Multiple sequence alignment used for model generation was done using MAFFT multiple sequence alignment tool  which employs fast fourier transforms (FFT) for rapid identification of homologous regions. The accuracy of alignments generated by MAFFT has been proved comparable to CLUSTALW and T-coffee progressive alignment methods with the rapid reduction of CPU time . BLAST Webserver at NCBI was used extensively for sequence searches against PDB database . PSORT II  was used for subcellular localization prediction analysis. The prediction accuracy for cross validation of yeast sequences is about 57%. PSORT II does not account for multiple localization of protein sequences. PSIPRED (Protein structure prediction server)  is used for secondary structural prediction of the leucyl tRNA synthetase of L. major. PFAM database  from the Sanger Institute, Conserved Domains Database (CDD Server) at NCBI  and SUPERFAMILY database (Version 1.75)  were used for domain assignments. BLAST searches against PDB database was used for the assignment of deacylase (Connective peptide; CP) domains for Leu, Ile and Val tRNA synthetases.
Phylogenetic analysis of the Lm aaRSs was performed combining the set of sequences from the Swissprot/UniprotKB database . Multiple sequence alignment (MSA) of these sequences is generated using CLUSTALW with default parameters . These MSAs were used as seed sequences for phylogenetic tree generation using Jones-Taylor-Thornton (JTT) model . MEGA v5  was used for both analysis and visualization of the phylogenetic trees.
Model building and validation
Comparative structural model of L. major Asparagine Synthetase A was built using Modeller v9 . Stereochemical quality of the model was verified using PROCHECK in PDBSUM web resource at EBI . Structural mapping of the active site residues was performed using Pymol .
Aminoacyl tRNA synthetases
Conserved domains database
Protein data bank.
RMB is a JC Bose National Fellow. VSG is currently a UGC D.S. Kothari Postdoctoral research fellow. This work is supported by DBT-COE postdoctoral research fellowship to VSG.
- Ibba M, Söll D: Aminoacyl-tRNA synthesis. Annu Rev Biochem. 2000, 69: 617-650. 10.1146/annurev.biochem.69.1.617.View ArticlePubMedGoogle Scholar
- Berriman M, Ghedin E, Hertz-Fowler C, Blandin G, Renauld H, et al: The genome of the African trypanosome Trypanosoma brucei. Science. 2005, 309: 416-422. 10.1126/science.1112642.View ArticlePubMedGoogle Scholar
- Charrière F, Helgadóttir S, Horn EK, Söll D, Schneider A: Dual targeting of a single tRNA(Trp) requires two different tryptophanyl-tRNA synthetases in Trypanosoma brucei. Proc Natl Acad Sci USA. 2006, 103: 6847-6852. 10.1073/pnas.0602362103.PubMed CentralView ArticlePubMedGoogle Scholar
- Merritt EA, Arakaki TL, Gillespie JR, Larson ET, Kelley A, et al: Crystal structures of trypanosomal histidyl-tRNA synthetase illuminate differences between eukaryotic and prokaryotic homologs. J Mol Biol. 2010, 397: 481-494. 10.1016/j.jmb.2010.01.051.PubMed CentralView ArticlePubMedGoogle Scholar
- Kwon NH, Kang T, Lee JY, Kim HH, Kim HR, et al: Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3. Proc Natl Acad Sci USA. 2011, 108: 19635-19640. 10.1073/pnas.1103922108.PubMed CentralView ArticlePubMedGoogle Scholar
- Park SG, Choi EC, Kim S: Aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMPs): a triad for cellular homeostasis. IUBMB Life. 2010, 62: 296-302.PubMedGoogle Scholar
- Bhatt TK, Khan S, Dwivedi VP, Banday MM, Sharma A, et al: Malaria parasite tyrosyl-tRNA synthetase secretion triggers pro-inflammatory responses. Nat Commun. 2011, 2: 530-View ArticlePubMedGoogle Scholar
- Hurdle JG, O'Neill AJ, Ingham E, Fishwick C, Chopra I: Analysis of mupirocin resistance and fitness in Staphylococcus aureus by molecular genetic and structural modeling techniques. Antimicrob Agents Chemother. 2004, 48: 4366-4376. 10.1128/AAC.48.11.4366-4376.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Hurdle JG, O'Neill AJ, Chopra I: Prospects for aminoacyl-tRNA synthetase inhibitors as new antimicrobial agents. Antimicrob Agents Chemother. 2005, 49: 4821-4833. 10.1128/AAC.49.12.4821-4833.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Olmedo-Verd E, Santamaría-Gómez J, Ochoa De Alda JA, Ribas De Pouplana L, Luque I: Membrane Anchoring of Aminoacyl-tRNA Synthetases by Convergent Acquisition of a Novel Protein Domain. J Biol Chem. 2011, 286: 41057-41068. 10.1074/jbc.M111.242461.PubMed CentralView ArticlePubMedGoogle Scholar
- Bhatt TK, Kapil C, Khan S, Jairajpuri MA, Sharma V, et al: A genomic glimpse of aminoacyl-tRNA synthetases in malaria parasite Plasmodium falciparum. BMC Genomics. 2009, 10: 644-657. 10.1186/1471-2164-10-644.PubMed CentralView ArticlePubMedGoogle Scholar
- Khan S, Sharma A, Jamwal A, Sharma V, Pole AK, et al: Uneven spread of cis- and trans-editing aminoacyl-tRNA synthetase domains within translational compartments of P. falciparum. Nature Scientific reports. 2011, 1: 1-11.Google Scholar
- Shibata S, Gillespie JR, Kelley AM, Napuli AJ, Zhang Z, et al: Selective inhibitors of methionyl-tRNA synthetase have potent activity against Trypanosoma brucei Infection in Mice. Antimicrob Agents Chemother. 2011, 55: 1982-1989. 10.1128/AAC.01796-10.PubMed CentralView ArticlePubMedGoogle Scholar
- Larson ET, Kim JE, Zucker FH, Kelley A, Mueller N, et al: Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate. Biochimie. 2011, 93: 570-582. 10.1016/j.biochi.2010.11.015.PubMed CentralView ArticlePubMedGoogle Scholar
- Larson ET, Kim JE, Castaneda LJ, Napuli AJ, Zhang Z, et al: The double-length tyrosyl-tRNA synthetase from the eukaryote Leishmania major forms an intrinsically asymmetric pseudo-dimer. J Mol Biol. 2011, 409: 159-176. 10.1016/j.jmb.2011.03.026.PubMed CentralView ArticlePubMedGoogle Scholar
- Wakasugi K, Schimmel P: Highly differentiated motifs responsible for two cytokine activities of a split human tRNA synthetase. J Biol Chem. 1999, 274: 23155-23159. 10.1074/jbc.274.33.23155.View ArticlePubMedGoogle Scholar
- Ibba M, Söll D: The renaissance of aminoacyl-tRNA synthesis. EMBO Rep. 2001, 2: 382-387.PubMed CentralView ArticlePubMedGoogle Scholar
- Geslain R, Aeby E, Guitart T, Jones TE, Castro De Moura M, et al: Trypanosoma seryl-tRNA synthetase is a metazoan-like enzyme with high affinity for tRNASec. J Biol Chem. 2006, 281: 38217-38225. 10.1074/jbc.M607862200.View ArticlePubMedGoogle Scholar
- Cassago A, Rodrigues EM, Prieto EL, Gaston KW, Alfonzo JD, et al: Identification of Leishmania selenoproteins and SECIS element. Mol Biochem Parasitol. 2006, 149: 128-134. 10.1016/j.molbiopara.2006.05.002.View ArticlePubMedGoogle Scholar
- Gurvitz A: Identification of the Leishmania major proteins LmjF07.0430, LmjF07.0440, and LmjF27.2440 as components of fatty acid synthase I. J Biomed Biotechnol. 2009, 2009: 950864-950872.PubMed CentralPubMedGoogle Scholar
- Nabholz C, Hauser R, Schneider A: Leishmania tarentolae contains distinct cytosolic and mitochondrial glutaminyltRNA synthetase activities. Proc Natl Acad Sci USA. 1997, 94: 7903-7908. 10.1073/pnas.94.15.7903.PubMed CentralView ArticlePubMedGoogle Scholar
- Rinehart J, Horn EK, Wei D, Soll D, Schneider A: Non-canonical eukaryotic glutaminyl- and glutamyl-tRNA synthetases form mitochondrial aminoacyl-tRNA in Trypanosoma brucei. J Biol Chem. 2004, 279: 1161-1166.View ArticlePubMedGoogle Scholar
- Nilsson D, Gunasekera K, Mani J, Osteras M, Farinelli L, et al: Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei. PLoS Pathog. 2010, 6: e1001037-10.1371/journal.ppat.1001037.PubMed CentralView ArticlePubMedGoogle Scholar
- Depledge DP, Evans KJ, Ivens AC, Naveed A, Asher M, Kaye PM, et al: Comparative expression profiling of leishmania: modulation in gene expression between species and in different host genetic backgrounds. PLoS Negl Trop Dis. 2009, 3: e476-10.1371/journal.pntd.0000476.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee JW, Beebe K, Nangle LA, Jang J, Longo-Guess CM, et al: Editing-defective tRNA synthetase causes protein misfolding and neurodegeneration. Nature. 2006, 443: 50-55. 10.1038/nature05096.View ArticlePubMedGoogle Scholar
- Sankaranarayanan R, Dock-Bregeon AC, Romby P, Caillet J, Springer M, et al: The structure of threonyl-tRNA synthetase-tRNA(Thr) complex enlightens its repressor activity and reveals an essential zinc ion in the active site. Cell. 1999, 97: 371-381. 10.1016/S0092-8674(00)80746-1.View ArticlePubMedGoogle Scholar
- Wolf YI, Aravind L, Grishin NV, Koonin EV: Evolution of aminoacyl-tRNA synthetases–analysis of unique domain architectures and phylogenetic trees reveals a complex history of horizontal gene transfer events. Genome Res. 1999, 9: 689-710.PubMedGoogle Scholar
- Guo M, Chong YE, Beebe K, Shapiro R, Yang XL, Schimmel P: The C-Ala domain brings together editing and aminoacylation functions on one tRNA. Science. 2009, 325: 744-747. 10.1126/science.1174343.PubMed CentralView ArticlePubMedGoogle Scholar
- Francin M, Kaminska M, Kerjan P, Mirande M: The N-terminal domain of mammalian Lysyl-tRNA synthetase is a functional tRNA-binding domain. J Biol Chem. 2002, 277: 1762-1769. 10.1074/jbc.M109759200.View ArticlePubMedGoogle Scholar
- Bilokapic S, Maier T, Ahel D, Gruic-Sovulj I, Söll D, et al: Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition. EMBO J. 2006, 25: 2498-2509. 10.1038/sj.emboj.7601129.PubMed CentralView ArticlePubMedGoogle Scholar
- Liu RJ, Tan M, Du DH, Xu BS, Eriani G, et al: Peripheral insertion modulates editing activity of the isolated CP1 domain of leucyl-tRNA synthetase. Biochem J. 2011, 440: 217-227. 10.1042/BJ20111177.View ArticlePubMedGoogle Scholar
- Seiradake E, Mao W, Hernandez V, Baker SJ, Plattner JJ, et al: Crystal structures of the human and fungal cytosolic Leucyl-tRNA synthetase editing domains: a structural basis for the rational design of antifungal benzoxaboroles. J Mol Biol. 2009, 390: 196-207. 10.1016/j.jmb.2009.04.073.View ArticlePubMedGoogle Scholar
- Zhao MW, Zhu B, Hao R, Xu MG, Eriani G, et al: Leucyl-tRNA synthetase from the ancestral bacterium Aquifex aeolicus contains relics of synthetase evolution. EMBO J. 2005, 24: 1430-1439. 10.1038/sj.emboj.7600618.PubMed CentralView ArticlePubMedGoogle Scholar
- Sokabe M, Okada A, Yao M, Nakashima T, Tanaka I: Molecular basis of alanine discrimination in editing site. Proc Natl Acad Sci USA. 2005, 102: 11669-11674. 10.1073/pnas.0502119102.PubMed CentralView ArticlePubMedGoogle Scholar
- Fukunaga R, Yokoyama S: Structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii. Acta Crystallogr D Biol Crystallogr. 2007, 63: 390-400. 10.1107/S090744490605640X.View ArticlePubMedGoogle Scholar
- Ahel I, Korencic D, Ibba M, Söll D: Trans-editing of mischarged tRNAs. Proc Natl Acad Sci USA. 2003, 100: 15422-15427. 10.1073/pnas.2136934100.PubMed CentralView ArticlePubMedGoogle Scholar
- Ruan B, Söll D: The bacterial YbaK protein is a Cys-tRNAPro and Cys-tRNACys deacylase. J Biol Chem. 2005, 280: 25887-25891. 10.1074/jbc.M502174200.View ArticlePubMedGoogle Scholar
- An S, Musier-Forsyth K: Trans-editing of Cys-tRNAPro by Haemophilus influenzae YbaK protein. J Biol Chem. 2004, 279: 42359-42362. 10.1074/jbc.C400304200.View ArticlePubMedGoogle Scholar
- Zhang H, Huang K, Li Z, Banerjei L, Fisher KE, et al: Crystal structure of YbaK protein from Haemophilus influenzae (HI1434) at 1.8 A resolution: functional implications. Proteins. 2000, 40: 86-97. 10.1002/(SICI)1097-0134(20000701)40:1<86::AID-PROT100>3.0.CO;2-Y.View ArticlePubMedGoogle Scholar
- Wong FC, Beuning PJ, Silvers C, Musier-Forsyth K: An isolated class II aminoacyl-tRNA synthetase insertion domain is functional in amino acid editing. J Biol Chem. 2003, 278: 52857-52864. 10.1074/jbc.M309627200.View ArticlePubMedGoogle Scholar
- Lam H, Oh DC, Cava F, Takacs CN, Clardy J, et al: D-aminoacids govern stationary phase cell wall remodeling in bacteria. Science. 2009, 325: 1552-1555. 10.1126/science.1178123.PubMed CentralView ArticlePubMedGoogle Scholar
- Miyoshi Y, Hamase K, Tojo Y, Mita M, Konno R, Zaitsu K: Determination of D-serine and D-alanine in the tissues and physiological fluids of mice with various D-amino-acid oxidase activities using two-dimensional high-performance liquid chromatography with fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009, 877: 2506-2512. 10.1016/j.jchromb.2009.06.028.View ArticlePubMedGoogle Scholar
- Bhatt TK, Yogavel M, Wydau S, Berwal R, Sharma A: Ligand-bound structures provide atomic snapshots for the catalytic mechanism of D-amino acid deacylase. J Biol Chem. 2010, 285: 5917-5930. 10.1074/jbc.M109.038562.PubMed CentralView ArticlePubMedGoogle Scholar
- Feng L, Tumbula-Hansen D, Toogood H, Söll D: Expanding tRNA recognition of a tRNA synthetase by a single amino acid change. Proc Natl Acad Sci USA. 2003, 100: 5676-5681. 10.1073/pnas.0631525100.PubMed CentralView ArticlePubMedGoogle Scholar
- Charron C, Roy H, Blaise M, Giegé R, Kern D: Non-discriminating and discriminating aspartyl-tRNA synthetases differ in the anticodon-binding domain. EMBO J. 2003, 22: 1632-1643. 10.1093/emboj/cdg148.PubMed CentralView ArticlePubMedGoogle Scholar
- Frechin M, Senger B, Brayé M, Kern D, Martin RP, Becker HD: Yeast mitochondrial Gln-tRNA(Gln) is generated by a GatFAB-mediated transamidation pathway involving Arc1p-controlled subcellular sorting of cytosolic GluRS. Genes Dev. 2009, 23: 1119-1130. 10.1101/gad.518109.PubMed CentralView ArticlePubMedGoogle Scholar
- Liao CC, Lin CH, Chen SJ: Trans-kingdom rescue of Gln-tRNAGln synthesis in yeast cytoplasm and mitochondria. 2012, Wang CC: Nucleic Acids Res, [Epub ahead of print]Google Scholar
- Roy H, Ibba M: Bridging the gap between ribosomal and nonribosomal protein synthesis. Proc Natl Acad Sci USA. 2010, 107: 14517-14518. 10.1073/pnas.1009939107.PubMed CentralView ArticlePubMedGoogle Scholar
- Sissler M, Delorme C, Bond J, Ehrlich SD, Renault P, et al: An aminoacyl-tRNA synthetase paralog with a catalytic role in histidine biosynthesis. Proc Natl Acad Sci USA. 1999, 96: 8985-8990. 10.1073/pnas.96.16.8985.PubMed CentralView ArticlePubMedGoogle Scholar
- Francklyn C: tRNA synthetase paralogs: evolutionary links in the transition from tRNA-dependent amino acid biosynthesis to de novo biosynthesis. Proc Natl Acad Sci USA. 2003, 100: 9650-9652. 10.1073/pnas.1934245100.PubMed CentralView ArticlePubMedGoogle Scholar
- Roy H, Zou SB, Bullwinkle TJ, Wolfe BS, Gilreath MS, et al: The tRNA synthetase paralog PoxA modifies elongation factor-P with(R)-β-lysine. Nat Chem Biol. 2011, 7: 667-669.PubMed CentralView ArticlePubMedGoogle Scholar
- Gilreath MS, Roy H, Bullwinkle TJ, Katz A, Navarre WW, et al: β-Lysine discrimination by lysyl-tRNA synthetase. FEBS Lett. 2011, 585: 3284-3288. 10.1016/j.febslet.2011.09.008.PubMed CentralView ArticlePubMedGoogle Scholar
- Ambrogelly A, O'Donoghue P, Söll D, Moses S: A bacterial ortholog of class II lysyl-tRNA synthetase activates lysine. FEBS Lett. 2010, 584: 3055-3060. 10.1016/j.febslet.2010.05.036.PubMed CentralView ArticlePubMedGoogle Scholar
- Yanagisawa T, Sumida T, Ishii R, Takemoto C, Yokoyama S: A paralog of lysyl-tRNA synthetase aminoacylates a conserved lysine residue in translation elongation factor P. Nat Struct Mol Biol. 2010, 17: 1136-1143. 10.1038/nsmb.1889.View ArticlePubMedGoogle Scholar
- Nakatsu T, Kato H, Oda J: Crystal structure of asparagine synthetase reveals a close evolutionary relationship to class II aminoacyl-tRNA synthetase. Nat Struct Biol. 1998, 5: 15-19. 10.1038/nsb0198-15.View ArticlePubMedGoogle Scholar
- Richards NG, Schuster SM: An alternative mechanism for the nitrogen transfer reaction in asparagine synthetase. FEBS Lett. 1992, 313: 98-102. 10.1016/0014-5793(92)81421-H.View ArticlePubMedGoogle Scholar
- Boehlein SK, Richards NG, Schuster SM: Glutamine-dependent nitrogen transfer in Escherichia coli asparagine synthetase B. Searching for the catalytic triad. J Biol Chem. 1994, 269: 7450-7457.PubMedGoogle Scholar
- Charron C, Roy H, Blaise M, Giegé R, Kern D: Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase. Acta Crystallogr D Biol Crystallogr. 2004, 60: 767-769. 10.1107/S0907444904003117.View ArticlePubMedGoogle Scholar
- Blaise M, Fréchin M, Oliéric V, Charron C, Sauter C, Lorber B, Roy H, Kern D: Crystal structure of the archaeal asparagine synthetase: interrelation with aspartyl-tRNA and asparaginyl-tRNA synthetases. J Mol Biol. 2011, 412: 437-452. 10.1016/j.jmb.2011.07.050.View ArticlePubMedGoogle Scholar
- Nakamura M, Yamada M, Hirota Y, Sugimoto K, Oka A, et al: Nucleotide sequence of the asnA gene coding for asparagine synthetase of E. coli K-12. Nucleic Acids Res. 1981, 9: 4669-4676. 10.1093/nar/9.18.4669.PubMed CentralView ArticlePubMedGoogle Scholar
- Aurrecoechea C, Brestelli J, Brunk BP, Fischer S, Gajria B, et al: EuPathDB: a portal to eukaryotic pathogen databases. Nucleic Acids Res. 2010, 38: D415-D419. 10.1093/nar/gkp941.PubMed CentralView ArticlePubMedGoogle Scholar
- Cusack S, Härtlein M, Leberman R: Sequence, structural and evolutionary relationships between class 2 aminoacyl-tRNA synthetases. Nucleic Acids Res. 1991, 19: 3489-3498. 10.1093/nar/19.13.3489.PubMed CentralView ArticlePubMedGoogle Scholar
- Sauter C, Lorber B, Cavarelli J, Moras D, Giegé R: The free yeast aspartyl-tRNA synthetase differs from the tRNA(Asp)-complexed enzyme by structural changes in the catalytic site, hinge region, and anticodon-binding domain. J Mol Biol. 2000, 299: 1313-1324. 10.1006/jmbi.2000.3791.View ArticlePubMedGoogle Scholar
- Laskowski RA: PDBsum new things. Nucleic Acids Res. 2009, 37: D355-D359. 10.1093/nar/gkn860.PubMed CentralView ArticlePubMedGoogle Scholar
- Aslett M, Aurrecoechea C, Berriman M, Brestelli J, Brunk BP, et al: TriTrypDB: a functional genomic resource for the Trypanosomatidae. Nucleic Acids Res. 2010, 38: D457-D462. 10.1093/nar/gkp851.PubMed CentralView ArticlePubMedGoogle Scholar
- Krogh A, Brown M, Mian IS, Sjölander K, Haussler D: Hidden Markov models in computational biology. Applications to protein modeling. J Mol Biol. 1994, 235: 1501-1531. 10.1006/jmbi.1994.1104.View ArticlePubMedGoogle Scholar
- Consortium UP: Ongoing and future developments at the Universal Protein Resource. Nucleic Acids Res. 2011, 39: D214-D219.View ArticleGoogle Scholar
- Jain E, Bairoch A, Duvaud S, Phan I, Redaschi N, et al: Infrastructure for the life sciences: design and implementation of the UniProt website. BMC Bioinformatics. 2009, 10: 136-154. 10.1186/1471-2105-10-136.PubMed CentralView ArticlePubMedGoogle Scholar
- Katoh K, Misawa K, Kuma K, Miyata T: MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 2002, 30: 3059-3066. 10.1093/nar/gkf436.PubMed CentralView ArticlePubMedGoogle Scholar
- Altschul SF, Wootton JC, Gertz EM, Agarwala R, Morgulis A, et al: Protein database searches using compositionally adjusted substitution matrices. FEBS J. 2005, 272: 5101-5109. 10.1111/j.1742-4658.2005.04945.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Horton P, Nakai K: Better prediction of protein cellular localization sites with the k nearest neighbors classifier. Proc Int Conf Intell Syst Mol Biol. 1997, 5: 147-152.PubMedGoogle Scholar
- Buchan DW, Ward SM, Lobley AE, Nugent TC, Bryson K, et al: Protein annotation and modelling servers at University College London. Nucl Acids Res. 2010, 38: W563-W568. 10.1093/nar/gkq427.PubMed CentralView ArticlePubMedGoogle Scholar
- Finn RD, Mistry J, Tate J, Coggill P, Heger A, et al: The Pfam protein families database. Nucleic Acids Res. 2010, 38: D211-D222. 10.1093/nar/gkp985.PubMed CentralView ArticlePubMedGoogle Scholar
- Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, et al: CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res. 2011, 39: D225-D229. 10.1093/nar/gkq1189.PubMed CentralView ArticlePubMedGoogle Scholar
- Gough J, Chothia C: SUPERFAMILY: HMMs representing all proteins of known structure. SCOP sequence searches, alignments and genome assignments. Nucleic Acids Res. 2002, 30: 268-272. 10.1093/nar/30.1.268.PubMed CentralView ArticlePubMedGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.PubMed CentralView ArticlePubMedGoogle Scholar
- Jones DT, Taylor WR, Thornton JM: The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci. 1992, 8: 275-282.PubMedGoogle Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, et al: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011, 28: 2731-2739. 10.1093/molbev/msr121.PubMed CentralView ArticlePubMedGoogle Scholar
- Eswar N, Eramian D, Webb B, Shen MY, Sali A: Protein structure modeling with MODELLER. Methods Mol Biol. 2008, 426: 145-159. 10.1007/978-1-60327-058-8_8.View ArticlePubMedGoogle Scholar
- DeLano WL: The PyMOL Molecular Graphics System. San Carlos, CA, USA: DeLano Scientific LLC
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.