Figure 9

Visualization of the interaction network of selected genes for which loss of function impacts the ionome. (A) The 33 genes that affect the abundance (increase and decrease) of P were selected and an interaction network built based on known protein protein and genetic interactions. There are 51 physical interactions and 36 genetic interactions among 26 of the 33 selected genes. (B) The 55 genes that affect the abundance (increase and decrease) of Mg were selected and an interaction network built based on known protein protein and genetic interactions. There are 39 physical interactions and 57 genetic interactions among 36 of the 55 selected genes. Protein protein and genetic interaction information were obtained from BioGRID [48]. Nodes represent genes, node color represents the direction of changes in elemental abundance (magenta increase in abundance, blue decrease in abundance), and node size represents the magnitude of the change in the ionome based on moderated Z-score. Lines joining the nodes (edges) in the graph represent the interactions. The color of the edges represents the direction of the correction (pink is positive and green negative) between the interacting pair of genes based on the ionomic profiles of the loss of function mutants. The width of the edges represents the strength of the correlation. A dotted edge represents a genetic interaction and solid edge represents a physical interaction.