Figure 1From: Construction of a highly flexible and comprehensive gene collection representing the ORFeome of the human pathogen Chlamydia pneumoniaeOverview of the construction of the C. pneumoniae ORFeome. The PCR products of the amplified ORFs were used in the first step of a BP reaction to create an Entry library, followed by PCR and sequencing for technical confirmation. For expression of recombinant proteins, the Entry library was used for LR reactions. Additionally, an Y2H library was generated for validation by functional analysis.Back to article page