Skip to main content

Table 4 Validation of selected microarray-based gene expression by real-time RT-PCR analysis

From: Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress

Probe Name

Description

Microarray

qRT-PCR

AGI Locus

Category

  

4 h/0 h

9 h/0 h

2 h/0 h

4 h/0 h

9 h/0 h

24 h/0 h

  

CUST_9007

JAZ7 (jasmonate-ZIM-domain protein 7)

-

3.92

2.34

1.96

3.75

2.82

AT2G34600

UK

CUST_5663

Unknown protein

1.69

3.92

1.20

1.53

2.63

4.19

  

CUST_5990

phosphoric monoester hydrolase

-

3.77

0.54

0.11

1.54

2.19

AT1G17710

UK

CUST_4383

gibberellin 2-beta-dioxygenase

-

3.63

5.80

3.66

4.02

4.62

AT1G30040

RS

CUST_13673

protein transmembrane transporter

-

3.61

1.41

0.20

1.71

2.08

AT4G16160

UK

CUST_16591

Unknown protein

2.28

3.53

1.41

1.62

1.34

2.61

AT5G37770

RS

CUST_11786

ubiquitin-protein ligase

-

3.48

1.14

1.15

1.35

1.91

AT1G68050

RS, CR

CUST_4231

sodium/calcium exchanger protein

-

3.11

0.35

0.60

1.87

1.32

AT1G53210

UK

CUST_20765

AP2 domain transcription factor

-

2.02

0.23

0.42

2.41

1.70

AT1G21910

TF

CUST_12461

C2H2-type zinc finger ZAT-12 like

-

1.97

1.23

1.39

1.10

2.57

AT2G28710

TF

CUST_19994

protein phosphatase 2C

-

1.71

0.68

0.39

1.12

0.28

AT3G12620

PM

CUST_3593

PCL1 (PHYTOCLOCK 1)

-

1.55

1.17

0.95

1.96

2.40

AT3G46640

TF

CUST_17985

TCH2 (calcium ion binding protein)

-

1.53

2.66

0.59

1.41

1.79

AT5G37770

PP

CUST_14166

GSTU19 (glutathione transferase)

-

-1.12

-0.82

-0.67

-1.05

-1.00

AT1G78380

RS, CH

CUST_1923

S-adenosylmethionine decarboxylase

-

-1.85

-0.91

-1.56

-1.89

-2.63

AT3G02470

AP

CUST_7527

Unknown protein

-1.47

-1.85

-0.43

-1.36

- 0.92

-1.06

 

UK

CUST_19213

Unknown protein

-1.64

-2.59

-0.77

-1.27

-1.28

-1.39

 

UK

CUST_5741

hypothetical protein

-2.61

-3.05

-1.94

-2.39

-3.04

-3.48

AT1G13360

UK

  1. Notes: All data are shown log2 Ratio, and positive and negative values of Log2 Ratio are either up- or down-regulated genes in the three pair-comparisons. No significant fold changes are indicated as "-". Values of real-time RT-PCR analysis showing in bold font indicate inconsistent data compared with microarray analysis. AP: 'Arginine and proline metablism', CH: 'Chloroplast', CR: 'Circadian rhythm-plant', PM: 'Protein metabolism', PP: 'Plant-pathogen interaction', RS: 'Response to abiotic and biotic stimulus' and 'Response to stress', TF: 'Transcription factor activity', UK: Unknown.