Figure 5

sgf73⁺, meu29⁺, sec65⁺and pab1⁺function in cytokinesis and DNA replication. (A) Microscopic analysis of deletions. Cells were stained with DAPI to visualize the nuclei. Cells exhibiting elongated cell length are indicated by arrows, multiple nuclei by asterisk, and abnormal septum by arrow heads. The bar represents 10 μm. (B) Increased expression levels of ace2⁺, agn1⁺ and eng1⁺ in the deletions. The mRNA levels were quantified by real time PCR and those of act1⁺ served as internal controls (n=3). The relative level in WT was designated as arbitrary unit 1. (C) Increased expression levels of cdc18⁺ and cdt1⁺ in deletions. Real time PCR was performed as described in Figure 5B (n=3).