Table 1 Selection of putative SCD containing Tel1/Mec1 targets

Bub1

Spindle assembly protein.

Cbf2

Essential kinetochore protein, component of the CBF3 multisubunit complex.

Cnn1

Kinetochore protein of unknown function.

Mad1

Spindle assembly protein.

Spc105

Subunit of a kinetochore-microtubule binding complex that bridges centromeric heterochromatin and kinetochore. Required for kinetochore binding of SAC proteins.

Tid3

Component of the evolutionarily conserved kinetochore-associated Ndc80 complex.

Mitotic spindle

Bub2

Mitotic exit network regulator.

Nud1

Component of the spindle pole body outer plaque, required for exit from mitosis.

Spc72

Binds spindle pole bodies and links them to microtubules.

Spc97

Interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque.

Spc110

Inner plaque spindle pole body (SPB) component.

Stu1

Component of the mitotic spindle that binds to interpolar microtubules.

Cytokinesis

Cdc3

Component of the septin ring of the mother-bud neck that is required for cytokinesis.

Cla4

Involved in septin ring assembly and cytokinesis.

Cts1

Required for cell separation after mitosis.

Dse4

Degrades cell wall from the daughter side causing daughter to separate from mother.

Egt2

Required for proper cell separation after cytokinesis.

Elm1

Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis.

Cell bud

Ace2

Transcription factor that activates expression of early G1-specific genes, localizes to daughter cell nuclei after cytokinesis and delays G1 progression in daughters.

Bud3

Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis.

Bud8

Protein involved in bud-site selection.

Num1

Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip.

She3

Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud.

Cortical Actin cytoskeleton

Akl1

Ser-Thr protein kinase involved in endocytosis and actin cytoskeleton organization.

Ark1

Serine/threonine protein kinase involved in regulation of the cortical actin cytoskeleton.

Bni1

Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation.

Las17

Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments.

Ndl1

Regulates dynein targeting to microtubule plus ends.

Sda1

Required for actin cytoskeleton organization.