Transcription analysis on response of porcine alveolar macrophages to Haemophilus parasuis

BMC Genomics

Table 3 Validation of microarray results by qPCR

Gene	Accession	Primers	Microarray fold change	qPCR fold change	p-value	Product size
*CD14 molecule* ^a	NM_213973.1	F: GCAGAGGCTTTGAGGACCTTATC	2.798496395	3.835	0.0001	154 bp
		R: GCTGCGGATGCGTGAAGTT
*CD3e molecule, epsilon (CD3-TCR complex)* ^a	NM_214227.1	F: ACCTCTTAGTTCCTCCCTTTG	5.671628855	4.619	0.005	137 bp
		R: TGCCAGCATTTACCCAGTC
*heat shock protein 70.2* ^b	NM_213766.1	F: AGGCGGAGAAGTACAAAGCG	3.374059696	5.775	0.007	257 bp
		R: GATGGGGTTACACACCTGCTC
*TIMP metallopeptidase inhibitor 1* ^b	NM_213857.1	F: CGCCTCGTACCAGCGTTAT	8.614065715	11.357	0.008	127 bp
		R: GTGGAAGTATCCGCAGACGC
*superoxide dismutase 2, mitochondrial (SOD2)* ^b	NM_214127.2	F: TCTGGACAAATCTGAGCCCT	2.647298815	2.1687	0.003	119 bp
		R: GACGGATACAGCGGTCAACTT
*S100A4*	XM_001929560.1	F: GTCCACCTTCCACAAGTA	5.120172033	5.4957	0.006	152 bp
		R: TGTCCAAGTTGCTCATCA
*S100A6*	NM_001044557.1	F: AAGGCTGATGGAAGACTT	3.082743701	5.5237	0.016	105 bp
		R: TTGAGGGCTTCATTGTAGA
*Caveolin 1*	NM_214438.2	F: CTTCACCACCTTCACTGT	4.301157871	2.319	0.041	184 bp
		R: GGAATAGACACGGCTGAT
*Caveolin 2*	NM_001123091.1	F: GCAGACAATATGGAAGAGTG	5.305246137	3.327	0.011	85 bp
		R: CAGGCTGACAGAAGAGAA
*coronin, actinbinding protein, 1A (CORO1A)*	BT025463.1	F: GTGGACTGGAGCCGAGATGGA	6.065784814	5.7667	0.0001	200 bp
		R: GCCACCTGCCGCTCACTC
*protein phosphatase1, regulatory (inhibitor) subunit 13 like (PPP1R13L)*	XM_002801296.1	F: CACCAGAGCAGCCGCAGAG	0.506516661	0.2116	0.0001	107 bp
		R: GTCCAGGAGGAGCACCAGAGG

ISSN: 1471-2164