Figure 8

Overview of de novo fatty acid and triacylglycerol(TAG) biosynthesis pathways. Numbers shown in red font correspond to unigenes encoding enzymes in the fatty acid (A) and TAG (B) biosynthesis pathways in Sacha Inchi seeds. Lipid substrates are abbreviated: 16C:0, palmitic acid; 18C:0, stearic acid; 18C:1, oleic acid; 18C:2, linoleic acid; 18C:3, linolenic acid. Enzyme/protein abbreviations are: α-PDHC, pyruvate dehydrogenase alpha subunit; β-PDHC, pyruvate dehydrogenase beta subunit; DHLAT, dihydrolipoamide acetyltransferase; LPD, dihydrolipoamide dehydrogenase; α-CT, carboxyltransferase alpha subunit; β-CT, carboxyltransferase beta subunit; BC, biotin carboxylase; BCCP, biotin carboxyl carrier protein; MAAT, malonyl-CoA ACP transacylase; ACP, acyl carrier protein; KAS I, II, III, ketoacyl-ACP synthase I, II, III; KAS, ketoacyl-ACP synthase II; HAD, hydroxyacyl-ACP dehydrase; KAR, ketoacyl-ACP reductase; EAR, enoyl-ACP reductase; SAD, stearoyl-ACP desaturase; FAD6, oleate desaturase (chloroplast); FAD7, linoleate desaturase (chloroplast); FAD2, oleate desaturase (endoplasmic reticulum); FAD3, linoleate desaturase (microsomal); FATA/B, acyl-ACP thioesterase A/B; PCH, palmitoyl-CoA hydrolase; LACS, long-chain Acyl-CoA synthetase; ACBP, acyl-CoA-binding protein; GPDH, NAD-dependent glycerol-3-phosphate dehydrogenase; CK, choline kinase; GPAT, glycerol-3-phosphate acyltransferase; LPAAT, 1-acylglycerol-3-phosphate acyltransferase; PAP, phosphatidic acid phosphatase; DGAT1/2, acyl-CoA: diacylglycerol acyltransferase 1/2; PLA2/C, phospholipase A2/C; LPCAT, 1-acylglycerol-3-phosphocholine acyltransferase; PDAT, phospholipid:diacylglycerol acyltransferase; CCT, cholinephosphate cytidylyltransferase; DAG-CPT, diacylglycerol cholinephosphotransferase; OBO, oil-body oleosin; STERO, steroleosin.