Strand-specific RNA-seq reveals widespread occurrence of novel cis- natural antisense transcripts in rice

BMC Genomics

Table 2 Numbers of cis- NATs with expression evidence and nat-siRNAs, under four different conditions and in epidermal cells

	Both expressed *cis* cbsgNAT pairs	Validated in epidermal cells	Pairs with siRNAs only in overlap region^f	Pairs with siRNAs enriched in overlap region^g
sd^a	1,789	1,043	72	9
ST^b	1,572	949	58	5
CD^c	1,668	1,003	66	5
DT^d	1,668	986	75	5
Co-	1,072 (46.7%)^e	725 (58.6%)^e	10 (5.9%)^e	2 (15.4%)^e
Total	2,292	1,238	166	13

^a 14-d-old-seedling.
^b 14-d-old-seedling treated with 200 mM NaCl.
^c 14-d-old-seedling grown under cold stress at 4°C for 24 h in darkness.
^d 14-d-old-seedling treated with 20% PEG-6000.
^e The percentage of co-cis- NATs in the corresponding total cis- NATs was calculated.
^f Small RNAs were only enriched in the overlapping regions with more than five reads.
^g The density of siRNAs in overlapping regions was > 5 times that in non-overlapping regions.

ISSN: 1471-2164