Figure 4

Detection of orb transcripts in sexual and asexual ovaries by fluorescent WISH. (A) orb riboprobes were detected with an antibody coupled to alexin fluorochrome (red) and DNA was stained with DAPI (white). In sexual ovaries (upper pictures), orb transcripts were detected at the basal part of the germaria (hollow arrowheads). In asexual ovaries (lower pictures), orb transcripts were detected in oocytes (white arrowheads) before ovulation (stage 0), and after ovulation (stage 1). (B) Actin filaments and DNA in sexual ovaries were stained with phalloidin (green) and propidium iodide (red), respectively. Cells at the basal part of the sexual germaria possibly correspond to presumptive sexual oocytes (double arrows). Orb sense riboprobes were used as negative controls. Bar scale: 30 μm.