Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2

Table 1 Top HopZ2^C/A Interactors

Locus	HopZ2 rpm¹	Luciferase rpm	Library rpm	HopZ2 Enrichment²	% cDNA Coverage	Additional Interactor³	Library⁴ or T-DNA Line⁵ Notes
At5g20700*	997.9	0	1.9	99	39		Exon
At4g35450	9,335.5	0	44.7	99	89		5' UTR
At5g13860	2,620.7	0	46.4	98	99		5' UTR
At5g37600	2,531.3	0	51	98	62	HopF2, ZAR1
At4g35750*	538.7	0	12.2	98	33		Exon
At3g55980	6,583.9	0	189	97	59	Z1b, HopF2, ZAR1
At3g55410	2,789	0	86	97	32		ND
At1g11310*	1,693.2	0	66.3	96	51		Exon
At3g04790	1,297.5	0	56.4	96	100	HopF2
At2g34470	3,792.6	1.5	184	95	100	ZAR1
At4g27520	1,945.6	0	101.2	95	71	Z3, ZAR1
At3g23890	0.6	0	0.03	95	2	Z3	Short
At3g17820	0.6	0	0.03	95	7		Short
At2g14720	2,792.8	0.5	161	95	37	HopF2
At2g05160	4,214.3	7.8	265.4	94	65		ND
At1g66200	1.9	0	0.14	93	6	ZAR1	Short
At1g01930	89.7	0.3	7.1	92	60	Z1b
At1g24706	195.6	0	18.4	91	13	Z1c
At1g68440*	185.8	0	18.2	91	21		Exon
At1g08980	7,293.2	8	701	91	100	Z1a, HopF2
At4g00430*	3,886.5	15.3	372.9	91	100		Exon
At2g04140	0.32	0	0.03	90	4	ZAR1	Short
At1g04170	0.32	0	0.03	90	6	Z3	Short
At5g67380	0.32	0	0.03	90	6		Short
At2g26110	0.32	0	0.03	90	4		Short
At5g54890	0.32	0	0.03	90	4	Z3	Short
At5g25110	0.32	0	0.03	90	3	Z1c	Short
At2g22840	0.32	0	0.03	90	5	Z1c	Short
At1g48280	0.32	0	0.03	90	5	Z1c	Short
At3g23390	0.32	0	0.03	90	19	Z1c, HopF2, ZAR1	Short
At5g24010	0.32	0	0.03	90	3	Z1c	Short
At3g55940	0.32	0	0.03	90	2	Z1b, Z1c, Z3, HopF2	Short
At1g66620	0.32	0	0.03	90	5	Z1a, Z1c, Z3	Short

¹ rpm = reads per million
² Enrichment = [(HopZ-Luciferase)/(HopZ+Luciferase+Library)]*100
³ See Additional file 5 for enrichment data with other baits.
⁴ "Short" indicates that the prey cDNA library clone was less 100 nt in length. ND = not determined.
⁵ "Exon" & "5' UTR" indicate that the T-DNA insertion is in either the exon or the 5' UTR.
*Tested for increased or decreased bacterial growth

ISSN: 1471-2164