Figure 1

Chondrogenic differentiation of hBM-MSCs in 3D scaffold. (A) Levels of gene expression over 3 weeks of chondrogenic differentiation for chondrogenic and MSC signature genes and control genes measured by microarray analysis. Shown are mean values for donors 2–4. Error bars are standard deviations (SD). (B) Immunofluorescence histochemical stainings for SOX9 and type II collagen (left) and aggrecan (right) after three weeks of chondrogenic differentiation in one representative donor (Donor 2). (C) Secretion of sulfated glycosaminoglycans (sGAG) during three weeks of culture in alginate discs in either normal culture medium (No diff) or in the chondrogenic differentiation medium. Data presented are mean values of technical quadruplicates for each of the four donors. Error bars show standard deviations of the technical replicates. (D) Microarray expression analysis of chondrogenic and mesenchymal signature genes and unchanged control genes. Log2 transformed mean values for change in expression between day 0 and day 7 of chondrogenic differentiation are shown relative to GAPDH expression. Shown are mean values of the results for the four donors (mean of biological quadruplicates). Error bars are SD