Figure 2

(A) Correlation between all combinations of pairs of donors for enriched histone marks. Each dot plot is a comparison between the level of enrichment in a given histone mark after 7 days of differentiation from two donors. The values in each plot are the number of reads observed in genome-wide 500 bp regions with enrichment of the histone mark in that particular region in cells from at least one of the two donors. Included within each dot plot is the Spearman's rho correlation coefficient. Results for H3K27ac for donor 1 are not available due to a failed experiment. (B) Heat-maps of changes in the course of 7 days of chondrogenic differentiation in promoter marks H3K27me3, H3K4me3 and H3K9ac. Rows represent 500 bp regions where at least one experiment produced at least 200 aligned reads. The columns show changes in the indicated histone modifications and are sorted using hierarchical clustering with the clusters represented at the top of the figure. The rows are sorted using Principal Component Analysis where the order of the rows is defined by the first two principal components. Colour bars at the bottom of the figure show the colour analogue to the log2 fold change in histone marking or gene expression. Each column has 96 013 rows