Figure 4

Profiling of epigenetic changes near signature genes. (A) Changes in histone marks for chondrogenic signature genes between day 0 and day 7 of chondrogenic differentiation. For H3K4me3, H3K27me3 and H3K9ac a 4 kb window around the TSS of all known transcripts was compared between the differentiation stages, while K36me3 reads were counted over the whole length of the longest known transcript. Shown is the log2 transformed histone enrichment ratio between differentiated cells and MSCs. Mean values of the results from the four donors (biological replicates) are shown. Error bars are SD. (B) Changes in histone marks for MSC signature genes between day 0 and day 7 of chondrogenic differentiation. Mean values of four donors with SD. (C) Changes in histone marks for unchanged control genes between day 0 and day 7 of chondrogenic differentiation. Mean values of four donors with SD