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Figure 4 | BMC Genomics

Figure 4

From: Double restriction-enzyme digestion improves the coverage and accuracy of genome-wide CpG methylation profiling by reduced representation bisulfite sequencing

Figure 4

Validation of methylation status of selected regions in promoters and the expression of their associated genes. (a-b) Methylation statuses of specific regions of two cell lines identified by Bisulfite genomic sequencing. Each circle represents one CpG site within the tested sequence: filled circles represent methylated CpGs while open circles represent unmethylated CpG sites. (a) Region located in the promoter region of DDIT4L gene. (b) Region located in the promoter region of SMG1 gene. (c) Gene expression levels in DKO relative to HCT116 cell line. The results are presented as real time PCR data, normalized by β-actin expression levels and digital gene expression sequencing (DGE) methods. Error bars denote S.D.

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BMC Genomics

ISSN: 1471-2164

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