Figure 2

Validation of biological and technical replicates using CummeRbund. The read numbers per transcript between the (A) biological and (B) technical replicates were compared. Since the read number per transcript (FPKM value) ranged from 0 to >10,000, the read number + 1 was transformed by log10. Each dot represents data from one transcript. There was a high correlation between the two sequencing results (r = 0.98, Pearson’s correlation; p < 0.001 two tail). Additional scatter plots for the other samples are shown in the Additional file 4.