Figure 2

Measurement of globin genes expression during human erythroid differentiation. (A) Expression of γ- and β-globin genes during erythroid differentiation of CD34⁺ cells derived from normal human PB (n=10). (B) Expression of γ-globin gene during erythroid differentiation of hematopoietic CD34⁺ progenitors of FL, CB, BM, PB and mPB origin (n=2-4). *P < 0.05 PB vs FL at day 6, **P < 0.01 CB vs. all other tissues at day 14. (C) Expression of β-globin gene during erythroid differentiation (n=2-4). **P < 0.01 FL-, CB-derived erythroid cells vs. all other cells at day 6, 10 and 14, except ^#P < 0.05 CB vs. mPB at day 6; *P < 0.05 PB vs. BM at day 14; ^##P < 0.01 mPB vs. BM. (D) Comparative expression of γ-globin genes of erythroid progenitor cells as measured by real time quantitative PCR and microarray analysis at day 6 of liquid culture (n=2-4). ). *P < 0.05 PB vs. FL by qPCR, ^#P < 0.05 BM vs. PB by microarray. Values are mean ± SEM.