Table 4 Primers used for qRT-PCR analysis
Test genes | Forward primers (5' - 3') | Reverse primers (5' - 3') | Product (bp) |
|---|---|---|---|
dpep1 | GACAACTGGCTGGTGGACA | ACCACACGCTGCCCAAA | 74 |
cldn1 | GCTGTCATTGGGGGTGCGAT | GGCAACTAAAATAGCCAGACCTGC | 54 |
Reference genes | |||
ubc | GGTCGCAGTTCTTGTTTGTGG | CACGAAGATCTGCATTGTCAAG | 59 |
b2m | TGCTGTCTCCATGTTTGATGTATCT | TCTCTGCTCCCCACCTCTAAGT | 86 |
atp5e | CTGGACTCAGCTACATCCGA | GCATCTCTCACTGCTTTTGCAC | 55 |