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Table 1 List of genes selected for differential expression analysis and the oligonucleotides used in this study

From: Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solanias a tool for biotechnological application

Putative function

Accessionnumber

E-Value

Protein ID*

qPCR forward (F) and reverse (R) primers (5' to 3')

acid sphingomielinase (asm)

JK840922

1.39E-136

548323

Forward: GCGAAGCATCTCGGCTATTGTAGT

Reverse: TCAAGTTGTGAACCGCTACTCGTC

β-1,3-endoglucanase (bgn)

JK840920

8.92E-21

241696

Forward: TCAACATCGCCAACGTCAACGAC

Reverse: TGCCAATACGGGAACCAGTGATC

chitinase 33 (chit)

JK840912

3e-51

387920

Forward: TGGAGCTCAACAGGCGCTGC

Reverse: ACGACGGCACTGCCAAAGGG

endochitinase 42 (endo)

JK840909

3.0E-46

364419

Forward: AAGGGTTACTACAGCTACAACGCC

Reverse: ACTTGAGGTAGGCAACCTTGGTGT

exo-rhamnogalacturonase (exo)

JK840947

2.12E-65

463001

Forward: TTACCTGAAGACATGGGCGGGAAT

Reverse: GCCTTCCGCCAATCAGCTTAACAT

glycosyl hydrolase (glyc)

JK840945

1.51E-09

199282

Forward: GAAATGTTGTCGTCACCAGACGGT

Reverse: GGCCGCGATTGCTGTTTCATAGT

amine oxidase (aoc)

JK840953

9.50E-15

538657

Forward: ATACACCCGAAGGAACCTTGTTGG

Reverse: TAGCGTGCCTCAATCTCCTTAGCA

phospholipase d (pld)

JK840907

3.58E-56

537712

Forward: TGGGAAGACGTTGCACACACAAAC

Reverse: AAATTGTCGTAGTCGTCCCAGGTG

checkpoint-like protein (chk1)

JK840936

2.48E-14

NA

Forward: TGCTGCCTTCCTTGGATGTAGTAG

Reverse: AAACATGGTGGCAACGGGTAACG

serine threonine-protein kinase (sck1)

JK840919

1.15E-80

480202

Forward: ATGCTGAAGAGCTTAAACGCCACC

Reverse: ACTTTGGCTTGAAGGGTGGAGAG

senescence-associated protein (sag)

JK840934

5.02E-30

547464

Forward: AGCTCACGTTCCCTATTAGTGGGT

Reverse: ATCCTTCGATGTCGGCTCTTCCTA

mbl2-like secreted (mbl2)

JK840948

2.05E-41

257052

Forward: TTGCTACGAGGGAGTTTGTTCCTG

Reverse: TGGAGTTGCACTGGTCTGAAGT

serine protease (ser)

JK840930

1.54E-12

366985

Forward: TGGAAGGGAGTGACCAAGCCTG

Reverse: GGAAAGGTCAGGAGTGCTATCGGG

aquaporin (aqp)

JK840978

1.47E-60

476226

Forward: GTTGATGGCATAACCAGTCTCCCA

Reverse: CAACAACATTGGAGCCGGAAACCT

duf895 domain membrane protein (duf)

JK8409133

1.67E-92

396055

Forward: TCCAATCCTTGCCGACGTAGTTGA

Reverse: TGCCAAGATCACATGGGTCGTTCT

peptide transporter (ptr2)

JK840963

8.11e-74

533699

Forward: AGTCATCTGGTTGTAGGCCAGGAA

Reverse: AAATTGTCGTAGTCGTCCCAGGTG

QID74 protein (qid)

JK840906

6.31E-113

456637

Forward: CAGAAGAAGTGCGTGTGCAACAAG

Reverse: AGCTAGCATCTTTGCCGCAGTTTG

eight cysteine-containing domain (cfem)

JK840940

1.09E-17

245062

Forward: GCGTCCGCAAAGAAACAACCTTCT

Reverse: AGAGAGCGGTGTTTGTAGCGATGA

Enolase (eno)

JK841017

3.54E-12

315824

Forward: ACTTTGACCGAGTCTATCCAGGCT

Reverse: ATACCGACGGAGATGTCAGCAATG

glyceraldehyde 3- phosphate dehydrogenase (gapd)

JK840981

1.32E-32

281265

Forward: CAGGTCGCCAAGAAGGTCATCATT

Reverse: AAGCGTTGGAGATGACATTGGCAC

pyruvate decarboxylase (pdc)

JK840980

2.60E-31

277935

Forward: GCAGGTGTTGGTCAATTCCTTCAG

Reverse: AACGCCAGATGGGAACTTGGTATC

heat shock protein (hsp98)

JK841007

9.91E-13

225475

Forward: TTGAGCGTCGTTTCCAACAGGTTC

Reverse: TGTCGAGAATGCTGACCTTGTGGT

zinc finger domain protein (c2h2)

JK841004

1.67E-15

543717

Forward: CAGACCTTGCACTTGTGCTTCTT

Reverse: AATGTTGTCGACCTCACTGCCT

hexose transporter-like protein (ht)

JK840987

1.20E-35

360713

Forward: GGAGTCCCATTTGCTCGAAGTGAT

Reverse: CGTGCTCATCGTCTTCTT CTTCGT

zinc-regulated transporter (zt)

JK840990

3.84E-15

295941

Forward: GGCCAAGAAATCCAGCAGGAAGAT

Reverse: TTGACTAGTGGCTGGGCTGAATAC

norsolorinic acid reductase (norA)

JK841015

6.41E-37

373869

Forward: ACCGTCTCGTCAACATGAGCTACT

Reverse: AAGTTCATGGAACCCAAGCACAGC

phosphatidylserine decarboxylase family protein (psd)

JK840982

4.91E-23

363281

Forward: TCTTTGAAGGCGTAGGTGATCCGA

Reverse: GCAGATCCGTAGAGGCAAGTATGT

coproporphyrinogen oxidase (cpox)

JK841003

4.88E-24

364552

Forward: TGATCCAGGCGTGTTCAGTCCAAA

Reverse: GCAGATCCGTAGAGGCAAGTATGT

α-tubulin

HS574101

  

Forward: TATCTGCTACCAGGCTCCCGAGAA

    

Reverse: TGGTGTTGGACAGCATGCAGACAG

  1. * BLASTX at http://genome.jgi.doe.gov/ using Trichoderma harzianum CBS 226.95 as a reference model.
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BMC Genomics

ISSN: 1471-2164

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