A combined strategy involving Sanger and 454 pyrosequencing increases genomic resources to aid in the management of reproduction, disease control and genetic selection in the turbot (Scophthalmus maximus)

Table 4 List of the top 20 deepest contigs originated from the 454 run of brain-hypophysis-gonad axis tissues of turbot ( S . maximus )

Contig number	Length	No. reads	Average coverage per nucleotide	% GC	Annotation	E-value	Accession Number	Database
1	744	1,997	578.7	53.7	40S ribosomal protein S9	8.00E-93	UniRef90_P46781	Uniref90
19	676	1,728	541.1	45.6	Parvalbumin	4.00E-34	UniRef90_B5WX08	Uniref90
1,926	101	466	398.6	43.6	Unknown
13	663	935	382.1	51.0	Nucleolar protein family A3	4.00E-25	UniRef90_A4IHX9	Uniref90
88	787	1,545	366.8	53.2	Similar to ribosomal protein S7	5.00E-98	UniRef90_UPI0000E801DC	Uniref90
44	701	930	336.4	56.0	Unknown
36	709	725	291.6	51.8	NADH dehydrogenase 1 beta	8.00E-75	UniRef90_C1BWY9	Uniref90
2	1,222	1,216	289.6	46.7	Chromobox protein homolog 3	3.00E-80	UniRef90_C3KJI6	Uniref90
6	1,103	1,335	285.0	48.4	Ribosomal protein L7	1.00E-108	dre:336710	KEGG
47	675	651	277.6	51.6	Fatty acid binding protein 11a	2.00E-49	dre:447944	KEGG
3	1,275	1,250	271.4	48.0	General transcription factor IIIA	6.00E-89	dre:445389	KEGG
92	603	687	271.2	51.9	Ribosomal protein S11	9.00E-73	UniRef90_B5FX82	Uniref90
9	759	929	270.6	50.8	Histone deacetylase complex	6.00E-74	UniRef90_C3KI01	Uniref90
179	649	738	266.8	50.0	Unknown
68	782	1,065	264.7	51.9	Ribosomal protein	1.00E-99	UniRef90_A9Z0M8	Uniref90
82	663	679	264.5	55.7	40S ribosomal protein S27a	4.00E-65	UniRef90_P68200	Uniref90
59	749	842	260.6	53.4	Ribosomal protein L12	6.00E-82	UniRef90_Q5BKW5	Uniref90
31	869	973	258.7	54.0	60S ribosomal protein L13	1.00E-101	UniRef90_B5DGD9	Uniref90
15	997	950	255.3	51.6	Ferritin	1.00E-90	UniRef90_Q4SBB8	Uniref90
4	1,346	1,340	253.2	42.6	Epididymal secretory protein E1	3.00E-70	UniRef90_C3KIM5	Uniref90

ISSN: 1471-2164