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Table 1 List of putative oocyst/sporozoite-specific proteins (POSPs) identified in this study

From: Global proteomic analysis of the oocyst/sporozoite of Toxoplasma gondiireveals commitment to a host-independent lifestyle

Gene ID

Protein name

EC number

Biological process/molecular function

aFold change of oocyst vs tachyzoite mRNA levels

Protein probability

    

day 0

day 4

day 10

 

energy

       

TGME49_026300

oxidoreductase, putative

1.3.1.34

fatty acid β-oxidation

260

40

103

6.23E-11

TGME49_034570

peroxisomal multifunctional enzyme type 2, putative

1.1.1.35

fatty acid β-oxidation

108

12

17

2.22E-16

TGME49_042390

enoyl-CoA hydratase/isomerase family protein, putative

4.2.1.17

fatty acid β-oxidation

21

16

5

1.35E-12

TGME49_047500

acyl-coenzyme A oxidase, putative

1.3.3.36

fatty acid β-oxidation

92

37

49

6.76E-12

TGME49_066270

2-methylbutyryl-CoA dehydrogenase, putative

1.3.99.-

fatty acid β-oxidation

7

<2

<2

1.00E-30

TGME49_115480

acyl-CoA oxidase, putative

1.3.3.36

fatty acid β-oxidation

183

71

151

1.91E-10

TGME49_032580

acetyl-coenzyme A synthetase, putative

6.2.1.1

glycolysis/gluconeogenesis

43

5

12

1.00E-30

TGME49_089930

phosphoenolpyruvate carboxykinase, putative

4.1.1.49

glycolysis/gluconeogenesis

30

3

6

8.70E-07

TGME49_016090

tropomyosin 1 alpha chain, putative

-

regulation of actin mechanics

247

11

19

5.66E-06

TGME49_027050

pyruvate dehydrogenase kinase, putative

2.7.11.2

regulation of pyruvate dehydrogenase

3

4

3

2.16E-07

TGME49_071210

4-alpha-glucanotransferase, putative

2.4.1.25/3.2.1.33

starch and glucose metabolism

5

254

26

1.11E-15

TGME49_003110

citrate synthase, putative

2.3.3.1

TCA cycle

35

5

7

6.32E-09

metabolism

       

TGME49_003340

alkyl sulfatase, putative

3.5.2.6

-

8

<2

2

2.10E-05

TGME49_003580

NAD-binding domain-containing protein

-

-

n.a.

n.a.

n.a.

4.73E-12

TGME49_038200

alpha/beta hydrolase fold domain containing protein

-

-

27

3

4

7.77E-15

TGME49_065860

nit protein 2, putative

-

-

5

<2

<2

2.74E-11

TGME49_069120

oxidoreductase, putative

1.1.1.100

-

183

61

125

4.44E-16

TGME49_069400

short chain dehydrogenase/reductase domain-containing protein

1.1.1.100

-

11

<2

<2

2.42E-07

TGME49_106450

short chain dehydrogenase family protein, putative

1.1.1.100

-

<2

222

82

6.97E-05

TGME49_111780

alcohol dehydrogenase class III, putative

1.1.1.284

-

4

<2

<2

4.61E-06

TGME49_003500

alanine dehydrogenase, putative

1.4.1.1

amino acid metabolism

6

29

4

1.11E-15

TGME49_005420

aspartate-semialdehyde dehydrogenase, putative

1.2.1.11

amino acid metabolism

4

<2

<2

2.44E-08

TGME49_016640

homoserine kinase, putative

2.7.1.39

amino acid metabolism

74

28

26

4.03E-12

TGME49_059180

cystathionine beta-synthase, putative

4.2.1.22

amino acid metabolism

n.a.

n.a.

n.a.

8.37E-13

TGME49_069110

ornithine aminotransferase, putative

2.6.1.13

amino acid metabolism

394

123

247

2.54E-12

TGME49_078910

O-acetylserine (thiol) lyase, putative

2.5.1.47

amino acid metabolism

20

3

5

2.39E-10

TGME49_112930

cystathionine beta-lyase, putative

4.4.1.1

amino acid metabolism

23

2

3

2.90E-10

TGME49_115260

alanine dehydrogenase, putative

1.4.1.1

amino acid metabolism

98

211

331

2.89E-14

TGME49_019230

acetoacetyl-CoA synthetase, putative

6.2.1.16

butanoate metabolism

<2

<2

<2

4.97E-10

TGME49_031900

acyl-CoA dehydrogenase, putative

1.3.99.7

fatty acid metabolism

31

32

21

6.51E-11

TGME49_003570

acyltransferase, putative

2.3.1.15

glycerophospholipid metabolism

2

<2

<2

3.08E-13

TGME49_010260

glycerol-3-phosphate dehydrogenase, putative

1.1.1.8

glycerophospholipid metabolism

188

14

32

6.84E-13

TGME49_082230

sulfate adenylyltransferas-adenylylsulfate kinase, putative

2.7.7.4

purine metabolism

52

5

10

7.38E-13

TGME49_107030

purine nucleoside phosphorylase

2.4.2.3

pyrimidine salvage pathway

12

2

3

1.11E-16

TGME49_003720

vitamin K epoxide reductase complex subunit 1, putative

1.1.4.1

terpenoid-quinone biosynthesis

9

21

23

1.63E-08

TGME49_002140

methylglutaconyl-CoA hydratase, putative

4.2.1.18

valine, leucine and isoleucine degradation

5

2

2

1.02E-06

TGME49_005430

isovaleryl-CoA dehydrogenase, putative

1.3.99.10

valine, leucine and isoleucine degradation

8

13

6

2.49E-10

TGME49_015250

thiamin pyrophosphokinase, putative

2.7.6.2

vitamin biosynthesis

<2

<2

<2

1.03E-09

cell rescue, defence and virulence

       

TGME49_005660

F5/8 type C domain-containing protein (CCp1A)

-

-

114

11

14

1.00E-30

TGME49_023480

thrombospondin type 1 domain-containing protein

-

-

24

<2

<2

6.35E-07

TGME49_056040

LCCL domain-containing protein (CCp5B)

-

-

343

24

32

1.11E-15

TGME49_058370

Rhoptry kinase family protein ROP28

-

-

<2

46

23

2.51E-12

TGME49_063410

scavenger receptor protein TgSR2

-

-

144

12

12

2.22E-15

TGME49_064070

antigenic protein, putative (CCp1B)

-

-

76

7

8

1.10E-10

TGME49_067410

scavenger receptor protein TgSR1

-

-

306

26

29

2.21E-08

TGME49_027150

glutaredoxin, putative

-

detoxification of oxygen radicals

-3

-3

-3

6.84E-05

TGME49_116190

superoxide dismutase, putative

1.15.1.1

detoxification of oxygen radicals

<2

589

470

3.76E-12

TGME49_009920

PAN domain-containing protein

-

host cell attachment/invasion

<2

557

142

2.33E-14

TGME49_032400

PAN domain-containing protein

-

host cell attachment/invasion

<2

44

8

7.77E-15

TGME49_038460

SRS22B

-

host cell attachment/invasion

<2

<2

<2

8.95E-05

TGME49_058550

SRS28 (SporoSAG)

-

host cell attachment/invasion

2

323

312

1.44E-14

TGME49_058810

SRS27B

-

host cell attachment/invasion

<2

153

46

4.84E-09

TGME49_065120

hypothetical protein, conserved (RON2 iso2)

-

moving junction formation

<2

28

13

8.17E-08

TGME49_094400

hypothetical protein (RON2 iso 1)

-

moving junction formation

n.a.

n.a.

n.a.

5.89E-06

TGME49_115730

apical membrane antigen, putative (AMA1 iso1)

-

moving junction formation

<2

16

4

4.98E-12

cell cycle and DNA processing

       

TGME49_006400

DNA photolyase, putative

4.1.99.3

DNA repair

34

252

99

1.82E-10

protein fate

       

TGME49_016150

oligoendopeptidase F, putative

-

-

213

25

52

1.21E-11

TGME49_031060

subtilase family serine protease, putative

3.4.21.66

-

<2

2

<2

3.95E-07

TGME49_034950

protein kinase (incomplete catalytic triad)

-

-

<2

2

5

2.22E-15

TGME49_044620

NEK kinase

2.7.12.2

-

2

<2

<2

1.11E-10

TGME49_046800

acylamino-acid-releasing enzyme, putative

3.4.19.1

-

<2

-2

<2

2.10E-10

TGME49_047240

ubiquitin carboxyl-terminal hydrolase isozyme L5, putative

3.4.19.12

deubiquitination pathway

3

-2

<2

7.70E-11

TGME49_066780

protein kinase (incomplete catalytic triad)

-

-

3

<2

<2

4.22E-14

TGME49_089240

DnaJ domain-containing protein

1.3.1.38

-

<2

<2

<2

4.52E-11

TGME49_095640

endothelin-converting enzyme, putative

3.4.24.71

-

27

3

4

3.79E-12

TGME49_107640

CMGC kinase, CK2 family

2.7.12.2

-

140

19

27

2.15E-12

TGVEG_058750

protein kinase domain-containing protein, putative

2.7.11.11

-

n.a.

n.a.

n.a.

8.47E-07

cellular transport, transport facilities and transport routes

       

TGME49_065230

electron transfer flavoprotein-ubiquinone oxidoreductase, putative

1.5.5.1

-

8

3

3

1.13E-12

TGME49_114330

ABC transporter, putative

3.6.3.44

-

31

13

12

9.50E-05

protein synthesis

       

TGME49_020300

ribosomal protein L15, putative

-

translation

<2

<2

<2

9.08E-05

biogenesis of cellular components

       

TGME49_043720

peroxisomal biogenesis factor 11 domain-containing protein

-

peroxisome fission

15

22

20

1.25E-05

TGME49_086250

oocyst wall protein, putative (OWP6)

-

oocyst environmental resistance

44

7

12

6.83E-10

other

       

TGGT1_064840

hypothetical protein

-

-

n.a.

n.a.

n.a.

7.85E-08

TGGT1_071710

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

5.11E-12

TGGT1_090830

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

2.89E-05

TGGT1_099390

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

7.99E-14

TGGT1_122010

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

6.77E-14

TGGT1_122320

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

4.09E-13

TGME49_002100

hypothetical protein

-

-

847

425

583

1.36E-07

TGME49_002110

hypothetical protein

-

-

1163

321

751

7.05E-11

TGME49_003230

hypothetical protein

-

-

11

42

27

3.05E-09

TGME49_003890

hypothetical protein

-

-

29

150

77

1.80E-09

TGME49_007250

hypothetical protein

-

-

155

15

28

7.55E-07

TGME49_009470

hypothetical protein

-

-

476

63

155

7.17E-10

TGME49_009810

hypothetical protein

-

-

<2

30

11

2.86E-12

TGME49_012030

hypothetical protein

-

-

<2

9

5

8.40E-11

TGME49_016970

hypothetical protein, conserved

-

-

-5

2

<2

1.44E-14

TGME49_020280

SCP-like domain-containing protein

-

-

n.a.

n.a.

n.a.

1.00E-30

TGME49_023430

hypothetical protein

-

-

<2

66

6

1.23E-09

TGME49_023830

fasciclin domain-containing protein

-

-

n.a.

n.a.

n.a.

1.50E-08

TGME49_023900

hypothetical protein

-

-

4

<2

<2

4.63E-05

TGME49_025120

rRNA processing protein, putative

-

-

n.a.

n.a.

n.a.

1.00E-09

TGME49_026230

hypothetical protein

-

-

<2

21

21

3.78E-08

TGME49_028240

hypothetical protein

-

-

<2

<2

<2

2.11E-13

TGME49_030530

hypothetical protein

-

-

220

22

33

1.99E-10

TGME49_030540

hypothetical protein

-

-

53

6

10

4.33E-08

TGME49_031390

hypothetical protein

-

-

618

69

138

1.46E-09

TGME49_037080

hypothetical protein

-

-

1315

582

905

1.56E-10

TGME49_038160

hypothetical protein

-

-

<2

252

78

1.03E-13

TGME49_038910

hypothetical protein

-

-

n.a.

n.a.

n.a.

3.50E-05

TGME49_040610

hypothetical protein, conserved

-

-

65

5

3

3.57E-08

TGME49_044410

hypothetical protein

-

-

n.a.

n.a.

n.a.

1.67E-07

TGME49_048170

hypothetical protein

-

-

<2

4

2

3.33E-14

TGME49_048230

hypothetical protein

-

-

<2

<2

<2

7.93E-05

TGME49_054760

hypothetical protein

-

-

153

126

80

1.12E-07

TGME49_055380

hypothetical protein

-

-

917

150

228

8.39E-12

TGME49_057690

hypothetical protein

-

-

16

3

3

2.10E-10

TGME49_058200

glutamic acid-rich protein, putative

-

-

<2

48

31

6.43E-09

TGME49_058380

hypothetical protein

-

-

<2

<2

<2

6.17E-05

TGME49_059670

von Willebrand factor type A domain-containing protein

-

-

<2

257

41

2.22E-15

TGME49_062470

beta antigen, putative

-

-

658

129

203

5.09E-05

TGME49_065130

hypothetical protein

-

-

<2

<2

<2

3.30E-11

TGME49_067980

hypothetical protein

-

-

<2

9

5

2.01E-07

TGME49_068880

hypothetical protein

-

-

-19

-3

-4

3.51E-09

TGME49_069350

hypothetical protein

-

-

<2

<2

<2

3.29E-11

TGME49_070950

hypothetical protein

-

-

15

238

348

5.74E-08

TGME49_071490

hypothetical protein

-

-

198

148

282

6.91E-13

TGME49_073510

hypothetical protein

-

-

<2

79

41

6.66E-15

TGME49_073670

hypothetical protein

-

-

-2

<2

<2

3.79E-05

TGME49_073700

hypothetical protein

-

-

260

27

52

3.42E-08

TGME49_076860

late embryogenesis abundant domain-containing protein

-

-

<2

556

390

2.22E-15

TGME49_076870

LEA1 protein, putative

-

-

8

497

146

2.03E-12

TGME49_078120

SCP-like extracellular domain-containing protein

-

-

<2

96

66

5.81E-09

TGME49_078340

hypothetical protein

-

-

2

4

<2

6.67E-07

TGME49_079440

hypothetical protein, conserved

-

-

161

14

26

4.43E-09

TGME49_080430

hypothetical protein

-

-

1057

220

376

1.47E-07

TGME49_085690

notch (DSL) domain-containing protein

-

-

121

15

21

8.51E-12

TGME49_087840

hypothetical protein

-

-

<2

<2

<2

3.00E-14

TGME49_088220

hypothetical protein

-

-

52

51

13

1.00E-30

TGME49_089740

hypothetical protein

-

-

<2

-4

<2

3.44E-07

TGME49_090610

hypothetical protein

-

-

557

38

42

5.26E-08

TGME49_091840

hypothetical protein

-

-

260

15

26

1.00E-30

TGME49_093780

hypothetical protein

-

-

-60

<2

<2

2.56E-06

TGME49_094600

hypothetical protein

-

-

15

506

557

3.19E-13

TGME49_097280

hypothetical protein, conserved

-

-

<2

453

139

2.63E-13

TGME49_097410

hypothetical protein

-

-

187

31

72

1.38E-08

TGME49_106050

hypothetical protein

-

-

89

14

30

1.11E-15

TGME49_106500

hypothetical protein

-

-

<2

<2

<2

6.28E-11

TGME49_109530

hypothetical protein

-

-

<2

3

<2

1.96E-11

TGME49_109540

hypothetical protein

-

-

<2

<2

<2

2.57E-10

TGME49_110160

piwi-PAZ domain-containing protein (TgAgo)

-

gene silencing

21

7

5

7.45E-12

TGME49_116890

hypothetical protein

-

-

46

5

9

5.18E-12

TGME49_119890

hypothetical protein

-

-

-14

53

33

1.08E-09

TGME49_120280

hypothetical protein

-

-

3

16

12

7.52E-12

TGME49_120290

hypothetical protein

-

-

8

<2

<2

4.68E-11

TGME49_120500

patched transmembrane domain-containing protein

-

-

12

<2

<2

1.37E-07

TGME49_120710

hypothetical protein

-

-

<2

9

4

2.15E-07

TGVEG_029770

hypothetical protein

-

-

n.a.

n.a.

n.a.

6.87E-06

TGVEG_051660

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

8.83E-13

TGVEG_058740

hypothetical protein, conserved

-

-

n.a.

n.a.

n.a.

2.22E-16

TGVEG_060360

hypothetical protein

-

-

n.a.

n.a.

n.a.

1.28E-11

TGVEG_095590

hypothetical protein

-

-

n.a.

n.a.

n.a.

3.27E-11

  1. aFold change values refer to differences between the mRNA levels of unsporulated (day 0) or sporulated (day 4 and day 10) oocysts compared to day 2 in vitro tachyzoites of strain M4 [34].
  2. Negative values indicate a downregulation in oocysts with respect to tachyzoites. Peak fold change values ≥2 are bolded. n.a., not available.
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