Table 1 Primers used in this study
From: Transcriptional analysis of the three Nlrp1 paralogs in mice
Name | 5′– 3′ sequence | Purpose |
|---|---|---|
Nlrp1a-1F | CAGAAGAGCGTCTTGAAGCAA | Sequencing of Nlrp1a |
Nlrp1a-2F | AAGCTTTACAGGAATGACTTCCAT | |
Nlrp1a-3F | CCAGCTCAGAGACCTCTGCT | |
Nlrp1a-4F | TTCAGCTGAACAGGAAGGTACAG | |
Nlrp1a-5F | GGATAAAGAAGAAGTGGGTGATAGC | |
Nlrp1a-6F | CTCCAAGAGGGAATCGTGG | |
Nlrp1a-7F | CTGCCCAAGATTGCTACAGC | |
Nlrp1a-1R | GAAGTCATTCCTGTAAAGCTTGC | |
Nlrp1a-2R | AGGGTCCTTCTTTGGCAGAC | |
Nlrp1a-3R | CCGTAAACTTATGTCCCTGCTG | |
Nlrp1a-4R | TCATCTTCAGTCCCCAGAGG | |
Nlrp1a-5R | AGTACTGCGTAATGCTGTTCCAC | |
Nlrp1a-6R | GCTTGTCCAAGAGAGGATCTACAG | |
Nlrp1a-7R | GTGCTCACTTTTCAGCCACC | |
Nlrp1a-F | ACAGACATGGACCTCATGGTGGTT | Survey of Nlrp1a expression – 201 bp [17] |
Nlrp1a-R | CAACTCCTCCAGGTTTCTGGCTAAC | Â |
Nlrp1b2-F | ATGTGATGGTATGGAATTCAAAAGAACTG | Survey of Nlrp1b (SV1, SV2) expression – 503 bp |
Nlrp1b2-R | AACCATACTGGGCAACTGTCAGCTT | Â |
Nlrp1b1-F | ACACGGTAATATGGAAATGGACAGAATTG | Survey of expression of all other Nlrp1b variants– 487 bp |
Nlrp1b1-R | AACCATCCTTGGGGATATCAGTGC | Â |
Nlrp1c-FW | GACAAGGGCAGTGACAATTGAGATT | Survey of Nlrp1c |
Nlrp1c-RV | ACATTTGGGGTCCTCAGTGTCACT | expression – 451 bp |
Actin-F | TACAGCTTCACCACCACAGC | beta-actin control in genomic DNA – 206 bp |
Actin-R | AAGGAAGGCTGGAAAAGAGC |