Figure 4

Presentation of TRACER data. (A) Summary display of results for a TRACER search. For each line the TRACER name, genomic position, orientation of the loxP site, summary of expression (expressed/positive, not expressed/negative, not done; developmental stage(s) assayed), status (alive, cryopreserved, not maintained, newly created) and the transposon type are displayed. The final two columns allow users to select the data for download, or to register interest in the line. Clicking on the “open” icon gives access to the detailed information of an insertion of interest. Quick access to a thumbnail photo of a representative embryo and to the corresponding transposon is possible by a simple click on the corresponding zone (B) Detailed view of a TRACER mouse line. The top section reveals basic information about the insertion, including the type of insertion, the name of the parent insertion, the orientation of the loxP site and a visual representation of the transposon in the correct orientation. The first panel shows the genomic context of the insertion in a snapshot from the Ensembl genome browser (Ensembl Genomic view +/−0.5 Mb) along with links to view the insertion point in the Ensembl or UCSC genome browsers. The second panel shows photos and annotations of the expression patterns of the regulatory sensor. The images can be mouse-overed to reveal a high-resolution zoomed-in view, and the annotation of expression domains is displayed. The third panel shows information related to mapping and genotyping, including the sequence obtained from the mapping procedure, and the sequences of the primers used to genotype animals carrying this specific insertion. The interface in the panel on the left allows neighbouring insertions to be selected for detailed analysis of a region’s regulatory potential.