Table 3 PCR primer and pooling factor information for the GS Junior HLA typing assay
Locus | Exon | Reference | Forward primer | Reverse primer | Amplicon length | Pool | Pooling Factor | ||
|---|---|---|---|---|---|---|---|---|---|
Region | Position | Region | Position | ||||||
A | 2 | A*01:01:01:01 | Exon 1 | 51-67 | Intron 2 | 66-83 | 576 | long | 1 |
A | 3 | A*01:01:01:01 | Intron 2 | 150-171 | Intron 3 | 65-83 | 521 | long | 1 |
A | 4 | A*01:01:01:01 | Intron 3 | 539-559 | Intron 4 | 76-91 | 541 | long | 1 |
B | 1 | B*07:02:01 | 5’NCR | 4998-5018 | Intron 1 | 45-59 | 278 | short | 0.5 |
B | 2 | B*07:02:01 | Intron 1 | 45-59 | Intron 2 | 25-43 | 467 | long | 1 |
B | 3 | B*07:02:01 | Intron 2 | 181-195 | Intron 3 | 36-52 | 462 | long | 1 |
B | 4 | B*07:02:01 | Intron 3 | 501-519 | Exon 5 | 27-45 | 558 | long | 1 |
C | 1 | C*01:02:01 | 5’NCR | 210-230 | Intron 1 | 42-62 | 279 | short | 0.5 |
C | 2 | C*01:02:01 | Intron 1 | 42-62 | Intron 2 | 42-58 | 487 | long | 1 |
C | 3 | C*01:02:01 | Intron 2 | 145-165 | Intron 3 | 65-83 | 531 | long | 1 |
C | 4 | C*01:02:01 | Intron 3 | 444-461 | Intron 4 | 76-94 | 584 | long | 1 |
C | 7 | C*01:02:01 | Intron 6 | 62-80 | Exon 8 | 14-33 | 366 | short | 0.5 |
DRB | 2 | DRB1*01:01:01 | Intron 1 | 5168-5189 | Exon 2 | 250-270 | 545 | long | 6 |
DRB | 3 | DRB1*01:01:01 | Intron 2 | 2143-2163 | Intron 3 | 1-20 | 459 | long | 1 |
DQB1 | 2 | DQB1*02:01:01 | Intron 1 | Exon 2 | 1429-1438 | 1-9 | Exon 2 | Intron 2 | 268-270 | 1-16 | 356 | short | 0.5 |
DQB1 | 3 | DQB1*02:01:01 | Exon 3 | 43-62 | Intron 3 | 1-27 | 357 | short | 0.5 |
DPB1 | 2 | DPB1*03:01:01 | Intron 1 | 4490-4517 | Intron 2 | 3-27 | 408 | long | 1 |