Table 2 Comparison of fold-changes in gene expression based on RNA-seq, microarray and real time qRT-PCR results
Gene symbol | Gene name | Gene description | RNA-seq fold-change | Array fold-change | Real time qRT-PCR fold-change |
|---|---|---|---|---|---|
CCL4 | Chemokine (C-C motif) ligand 4 | A proinflammatory and chemotactic chemokine | 40.02 | 10.53 | 26.51 |
CCL5 | Chemokine (C-C motif) ligand 5 | A proinflammatory chemokine involved in the chemotaxis of monocytes and T-helper cells | 17.43 | 14.68 | 19.12 |
CCL20 | Chemokine (C-C motif) ligand 20 | A chemokine involved in the chemoattraction of lymphocytes and neutrophils | 67.34 | 82.33 | 45.94 |
CD40 | CD40 molecule, TNF receptor superfamily member 5 | A member of the TNF-receptor superfamily. Mediates the immune and inflammatory responses | 13.43 | 9.99 | 12.32 |
CFB | Complement factor B | A component of the alternative pathway of complement activation | 3.87 | 40.63 | 40.36 |
CXCL2 | Chemokine (C-X-C motif) ligand 2 | An immunoregulatory chemokine produced by activated monocytes and neutrophils at sites of inflammation | 15.72 | 4.44 | 14.12 |
IL15 | Interleukin 15 | A cytokine that regulates T and natural killer cell activation and proliferation | 3.07 | Not DE | 2.23 |
IL1B | Interleukin 1, beta | A cytokine that mediates the inflammatory response including cell proliferation, differentiation and apoptosis | 34.84 | 92.28 | 41.21 |
IL6 | Interleukin 6 | A cytokine that functions in inflammation and the maturation of B cells | 39.62 | 88.47 | 55.08 |
IRF1 | Interferon regulatory factor 1 | A member of the interferon regulatory transcription factor family. An activator of interferon alpha and beta transcription | Not DE | 10.01 | 9.74 |
NFKB2 | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) | A pleiotropic transcription factor involved in inflammation, immunity, differentiation, cell growth and apoptosis | 5.35 | 8.68 | 5.76 |
TNF | Tumor necrosis factor (TNF superfamily, member 2) | A proinflammatory cytokine (secreted by macrophages) involved in the regulation cell proliferation, differentiation and apoptosis, and coagulation. | 15.16 | 80.07 | 15.00 |
AREGB | Amphiregulin B | A growth-modulating glycoprotein | −3.84 | −9.70 | −5.09 |
FOS | FBJ murine osteosarcoma viral oncogene homolog | A leucine zipper protein member of the AP-1 transcription factor complex. | −3.69 | −5.50 | −3.61 |
PIK3IP1 | Phosphoinositide-3-kinase interacting protein 1 | Suppresses the activity of phosphatidylinositol-3-kinase (PI3K), a regulator of cell division | −2.06 | −1.68 | Not DE |
SPRY2 | Sprouty homolog 2 (Drosophila) | An inhibitor of receptor tyrosine kinase signalling proteins | −2.12 | −4.64 | Not DE |