Figure 1

Carrier-optimized ChIP-seq in MCF7 cells. A. ChIP-qPCR on pS2/TFF1 promoter for ERα with differential carrier conditions (none, glycogen, mRNA/histones, glycogen/mRNA/histones). Data were normalized over negative control region and input. Bars show standard deviation from triplicate measurements. B. Genome browser snapshot of ERα ChIP-seq, depicting the pS2/TFF1 promoter and enhancer region for 10,000 cells without (top) or with mRNA/histones carrier (middle). Data was compared to a saturated ERα ChIP-seq from 20*10⁶ cells (bottom). Genomic coordinates and tag count are indicated. C. Heatmap visualization of ERα ChIP-seq data, depicting all binding events centred on the peak region within a 5 kb window around the peak. All peaks for the 20*10⁶ cells ChIP-seq condition were ranked on intensity, and the data from both 10,000 cells-conditions were plotted in identical order.