Figure 2

Example fluorescence micrographs for expression of crh-2::gfp fusions. A. An L2 larva of strain UL3706 with broad reporter expression for a fusion tagging transcript a. B. An L4 larva of strain UL3709 with reporter expression in the seam cells (nuclei arrowed) for a fusion tagging transcript b. C. An adult and an L4 larva of strain UL3809 with reporter expression in the intestinal nuclei for a fusion tagging transcript c. D. Two L1 larvae of strain UL3808 with broad reporter expression for a fusion tagging all three transcripts. The GFP is nuclear localized for each fusion. In C the fluorescence is superimposed upon the corresponding DIC micrograph. All images captured at 400x magnification.