Figure 4

Differentially expressed genes (DEGs). A-B. Distance between genes and genomic scars. The distance from each gene to the nearest genomic scar resulting from genome reduction is plotted. The genes with conserved (nonDEGs), increased, or reduced expression caused by genome reduction are shown in gray, orange, and purple, respectively (A). The genes with conserved (nonDEGs), upregulated, or downregulated expression due to heat shock are shown in gray, yellow and cyan, respectively (B). All the genes of the DEGs_gr and DEGs_hs are shown in red and brown, respectively. Significance was determined with the Wilcoxon-Mann–Whitney test, and the P-values are indicated. C. Enriched gene categories. The DEGs were divided into 23 gene categories, according to a previous report [37]. One-tailed binominal tests were performed to determine the significance of the enrichment of each category. The log-scale P-values are displayed as a heat map with gradations of blue; darker blue indicates higher significance.