Figure 5
Phylogeny of A. chinensis and A. eriantha MYB genes evaluated from protein sequence. (a). Phylogeny of MYB proteins implicated in anthocyanin regulation in various genera, and the positions assumed by the MYB10 and MYB110 of Actinidia chinensis var. chinensis and A. eriantha. Phylogenetic and molecular evolutionary analysis was conducted using MEGA version 4.0.2 [38] (using minimum evolution phylogeny test and 1000 bootstrap replicates). GenBank numbers; RhMYB10 (ABX79949), ZmP (AF292540), ZmC1 (Y15219), IbMYB1 (AB576767), GmMYB10 (ACM62751), ROSEA1 (DQ275529), MYB1 bayberry (GQ340767), VvMYBA2 (ABL14065), VlMYBA2 (BAC07540), VvMYBA1 (AB242302), ANT1 (AY348870), PhAn2 (EF423868), CaA (AJ608992), FvMYB10 (ABX79948), FaMYB10 (ABX79947), PpMYB10 (ABX79945), PavMYB10 (ABX71493), MdMYB110 (EB710109), EjMYB10 (ABX71484), CoMYB10 (ABX71483), MdMYB10 (ACQ45201), MdMYB1 (DQ886414) and c-MYB (AAB49039). (b). Protein sequence data from which phylogenetic relationships of MYB genes were evaluated. Sequences were aligned using Clustal W (opening = 15, extension-0.3) in Vector NTI9.0. Arrows indicate the amino acid signature motif ([DE]Lx2[RK]x3Lx6Lx3R) in the R2R3 domain, which allows interaction with a bHLH partner, and the box shows the C-terminus motif typical of anthocyanin-related regulators.