Figure 6

Magnitude of effect from exposure to 15 μM dichlorvos increases with time. A) The Venn diagram of the probe sets grouped by harvest time illustrates the overlap in gene expression and increasing number of differentially expressed genes at later harvest times. B) A principal components analysis of the 2259 probe sets highlights the large exposure and developmental effects. Washout samples exposed for only 8 h return to baseline. Concentrations are indicated by shapes and harvest times are indicated by color. Large circles indicate the centroid for each condition. PC2 is well correlated with time of development. C) A heatmap of 2259 differentially expressed probe sets shows changes (log₂ ratio) in gene expression in worms exposed to 15 μM dichlorvos versus unexposed worms at the same predicted ages. Continuous and 8-h washout (Wash) conditions are shown.