Bacillus subtilis genome vector-based complete manipulation and reconstruction of genomic DNA for mouse transgenesis

Table 1 Summary of the read mapping in DNA sequencing

	BAC1		BAC2		Tg110CIBS		Tg220CISP		Tg250SB
	# reads	%	# reads	%	# reads	%	# reads	%	# reads	%
Total reference length	114,183		220,003		115,372		222,532		254,658
Mapped reads	8,341,236	91.1	8,604,424	95.9	1,028,479	2.7	1,737,127	5.2	504,510	5.8
Unmapped reads	814,824	8.9	370,790	4.1	37,166,207	97.3	31,564,383	94.8	8,227,736	94.2
Reads in pairs	8,327,110	91.0	8,594,722	95.8	960,578	2.5	1,714,062	5.2	498,152	5.7
Broken paired reads	14,126	0.2	9,702	0.1	67,901	0.2	23,065	0.1	6,358	0.1
Total number of reads	9,156,060		8,975,214		38,194,686		33,301,510		8,732,246
Total read length (Mb)	915.6		897.5		3,819.5		3,330.2		873.2
Fraction of reference covered	1.00		1.00		1.00		1.00		1.00
Minimum coverage	241		125		28		22		6
Maximum coverage	15147		5631		2211		1514		574
Average coverage	7100.7		3812.3		851.5		752.2		191.0

Reads obtained from the Illumina Genome Analyzer IIx were analyzed and mapped to each reference sequence using CLC Genomics Workbench 5.5 (CLC Bio). The number of mapped reads in each sample was enough to evaluate mutations. Analyzed DNA samples are follows: original BAC clones of BAC1 and BAC2 and BGM clones of Tg110CIBS, Tg220CISP and Tg250SB.

ISSN: 1471-2164