Figure 2

Differential CREB binding analysis. A) Venn diagram of high-confidence CREB site calls in the re-fed (blue) and fasted (red) mouse liver suggests thousands of condition-specific binding events. B) Flowchart of quantitative analysis. C) Scatter-plot of average peak height (RPM = reads per million) across all fasted and re-fed replicates, for 7,547 merged peaks. Black dots correspond to peaks called in both conditions, red dots correspond to peaks called only in the fasted group, and blue dots correspond to peaks called only in the re-fed group. Gray error bars indicate SEM. D) Boxplot of log2 fasted/re-fed ratio of peak height for subsets of CREB binding sites based on occurrence of canonical full or half CRE sequence within 50 bp of peak center. E) ChIP-qPCR of CREB binding in the re-fed (blue) and fasted (red) groups on known fasting-induced CREB target genes. Neither change is significant at p-value of 0.05 threshold (one-tailed Student’s t-test).