Figure 5

Enrichment of CREB binding around fasting-responsive genes. A) Heatmap of genes called as differentially expressed in fasted versus re-fed liver microarray experiment. Known fasting-inducible CREB targets are shown on top right. Selected feeding-inducible and ER stress genes are shown at bottom right. Number of genes and associated CREB sites are shown on left. B) For each gene category (fasting-induced in red, fasting-repressed in green, and all genes on the microarray in gray), we computed the percentage of genes containing 1, 2, or 3+ high-confidence CREB binding sites anywhere in the 10 kb TSS upstream region or the gene body (including introns). ~60% of regulated genes (both fasting-induced and fasting-repressed) have at least one CREB binding site by this criteria, while 33% of all genes on the array can be associated with at least one CREB binding site. *p-value < 1E-5 by Fisher’s Exact Test versus gray bars. NS indicates p-value > 0.05. A full listing of differentially expressed genes and associated CREB binding is provided in Additional file 2.