Figure 2

Phylogenetic relationship among the glycosyl hydrolase 35 proteins of flax other species. Deduced amino acid sequences were aligned with MUSCLE [25]. The tree was created with GARLI [28], using the maximum likelihood method, following the WAG model of amino acid substitutions [27]. A consensus tree of 1000 bootstrap replicates was produced for which percent reproducibility under 100 is shown. The flax sequences are named LuBGAL, and numbered according to Tables 1 and 2. Arabidopsis thaliana sequences are indicated as AtBGAL, and numbered according to existing designations [23]. Oryza sativa sequences are indicated as OsBGAL, and numbered according to existing designations [24]. Physcomitrella patens sequences are indicated as PpBGAL, Populus trichocarpa sequences are indicated as PtBGAL, and Ricinus communis sequences are indicated as RcBGAL. Genomic loci corresponding to these sequences are presented in Table 1. A human beta-galactosidase (GLB1; NP_000395) was used to establish the outgroup.